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葡聚糖凝胶SP-C25

葡聚糖凝胶SP-C25结构式
葡聚糖凝胶SP-C25结构式
品牌特惠专场
常用名 葡聚糖凝胶SP-C25 英文名 sp sephadex
CAS号 61840-62-8 分子量 N/A
密度 N/A 沸点 N/A
分子式 N/A 熔点 N/A
MSDS 美版 闪点 N/A

Post-translational processing of oxytocin-neurophysin prohormone in the ovine corpus luteum: activity of peptidyl glycine alpha-amidating mono-oxygenase and concentrations of its cofactor, ascorbic acid.

J. Endocrinol. 122(1) , 313-322, (1989)

Peptidyl glycine alpha-amidating mono-oxygenase (PGA), the terminal enzyme in the pathway of oxytocin synthesis, was measured in extracts of ovine corpora lutea throughout the oestrous cycle. Activity of PGA was low early in the cycle but increased between da...

Affinity-specific protein separations using ligand-coupled particles in aqueous two-phase systems: I. Process concept and enzyme binding studies for pyruvate kinase and alcohol dehydrogenase from Saccharomyces cerevisiae.

Biotechnol. Bioeng. 33(9) , 1081-1088, (1989)

A process using ligand-coupled particles in aqueous polyethylene glycol-dextran two-phase polymer systems was developed to achieve a highly selective, scaleable biochemical separation process. Product protein is bound to the ligand-coupled particles that quan...

Preliminary characterization of the acrasin of the cellular slime mold Polysphondylium violaceum.

Proc. Natl. Acad. Sci. U. S. A. 73(3) , 795-799, (1976)

Some species of cellular slime mold do not respond to cyclic AMP as an acrasin, or chemoattractant. In one such species, Polysphondylium violaceum, we have isolated and purified its acrasin and determined some of its chemical properties, which lead us to beli...

Mouse liver metallothioneins. Complete amino acid sequence of metallothionein-I.

J. Biol. Chem. 252(22) , 8217-8221, (1977)

The amino acid sequence of thionein-I, a major mouse liver thionein, is presented. Thionein-I is a single chain polypeptide which consists of 61 amino acid residues and has the amino acid composition Cys20, Asp3, Asn1, Thr5, Ser9, Gln1, Pro2, Gly5, Ala5, Val2...

Purification of microsomal signal peptidase as a complex.

Proc. Natl. Acad. Sci. U. S. A. 83(3) , 581-585, (1986)

We report here the purification to near homogeneity of signal peptidase from canine pancreatic microsomes. Purification was monitored using an improved post-translational assay. A 42-fold enrichment over starting membranes was achieved by selective solubiliza...

Enkephalin inhibits release of substance P from sensory neurons in culture and decreases action potential duration.

Proc. Natl. Acad. Sci. U. S. A. 76(1) , 526-530, (1979)

Sensory neurons grown in dispersed cell culture in the absence of non-neuronal cell types contain immunoreactive substance P that is chemically similar to synthetic substance P. When depolarized in high-K+ media (30-120 mM), the neurons release this peptide b...

Separation and determination of thiamin and its phosphate esters by SP-Sephadex chromatography.

Meth. Enzymol. 122 , 20-24, (1986)

Structures of viscotoxins A1 and B2 from European mistletoe solved using native data alone.

Acta Crystallogr. D Biol. Crystallogr. 64(Pt 9) , 985-992, (2008)

Crystals of the cytotoxic thionin proteins viscotoxins A1 and B2 extracted from mistletoe diffracted to high resolution (1.25 and 1.05 A, respectively) and are excellent candidates for testing crystallographic methods. Ab initio direct methods were only succe...

Regulation of a cytosolic and nuclear O-GlcNAc transferase. Role of the tetratricopeptide repeats.

J. Biol. Chem. 274(45) , 32015-32022, (1999)

The O-GlcNAc transferase (OGT) is a unique nuclear and cytosolic glycosyltransferase that contains multiple tetratricopeptide repeats. We have begun to characterize the mechanisms regulating OGT using a combination of deletion analysis and kinetic studies. He...

Glycosylation of nuclear and cytoplasmic proteins. Purification and characterization of a uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase.

J. Biol. Chem. 267(13) , 9005-9013, (1992)

Using a combination of conventional and affinity chromatographic techniques, we have purified a uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase (O-GlcNAc transferase) over 30,000-fold from rat liver cytosol. The transfer...