Luciferase
Luciferase structure
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Common Name | Luciferase | ||
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| CAS Number | 9014-00-0 | Molecular Weight | 213.2304 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C14H17NO6 | Melting Point | N/A | |
| MSDS | Chinese USA | Flash Point | N/A | |
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The real-time-based assessment of the microbial killing by the antimicrobial compounds of neutrophils.
ScientificWorldJournal 11 , 2382-90, (2011) A recombinant Escherichia coli K-12 strain, transformed with a modified bacterial luciferase gene (luxABCDE) from Photorhabdus luminescens, was constructed in order to monitor the activity of various antimicrobial agents on a real-time basis. This E. coli-lux... |
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Differential analysis of bactericidal systems of blood serum with recombinant luminescent Escherichia coli and Bacillus subtilis strains.
Bull. Exp. Biol. Med. 154(1) , 59-63, (2012) Luminescence intensity of recombinant Escherichia coli and Bacillus subtilis strains with cloned luxCD(AB)E genes of the natural luminescent microorganism Photobacterium leiognathi was studied under the influence of 30 individual samples of human blood serum ... |
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Sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.
Arch. Microbiol. 183 , 203-8, (2005) Recent studies indicated that bioluminescence of the marine bacterium Vibrio harveyi may both stimulate DNA repair and contribute to detoxification of deleterious oxygen derivatives. Therefore, it was also proposed that these reactions can be considered biolo... |
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Effect of pH, EDTA, and anions on heavy metal toxicity toward a bioluminescent cyanobacterial bioreporter.
Arch. Environ. Contam. Toxicol. 57(3) , 477-87, (2009) The bioavailability and therefore toxicity of a metal depends on the chemical species present in a particular environment. We evaluated the effect of a series of factors that could potentially modify metal speciation on the toxicity of Hg, Cu, Zn, and Cd towa... |
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Crystal structure of the bacterial luciferase/flavin complex provides insight into the function of the beta subunit.
Biochemistry 48(26) , 6085-94, (2009) Bacterial luciferase from Vibrio harveyi is a heterodimer composed of a catalytic alpha subunit and a homologous but noncatalytic beta subunit. Despite decades of enzymological investigation, structural evidence defining the active center has been elusive. We... |
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Stereochemical studies of the type II isopentenyl diphosphate-dimethylallyl diphosphate isomerase implicate the FMN coenzyme in substrate protonation.
ChemBioChem. 13(1) , 42-6, (2012)
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Fre Is the Major Flavin Reductase Supporting Bioluminescence from Vibrio harveyi Luciferase in Escherichia coli.
J. Biol. Chem. 284(13) , 8322-8, (2009) Unlike the vast majority of flavoenzymes, bacterial luciferase requires an exogenous source of reduced flavin mononucleotide for bioluminescence activity. Within bioluminescent bacterial cells, species-specific oxidoreductases are believed to provide reduced ... |
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The fusion Vibrio campbellii luciferase as a eukaryotic gene reporter.
J. Biotechnol. 162(2-3) , 346-53, (2012) Bacterial luciferase from Vibrio campbellii is a thermostable enzyme with an in vitro thermal inactivation half-life of ~1020 min at 37°C. The enzyme also binds tightly to reduced FMN. In this study, a V. campbellii fusion luciferase construct in which the α ... |
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Analysis of the Bacterial Luciferase Mobile Loop by Replica-Exchange Molecular Dynamics
Biophys. J. 99(12) , 4012-9, (2010) Bacterial luciferase contains an extended 29-residue mobile loop. Movements of this loop are governed by binding of either flavin mononucleotide (FMNH 2) or polyvalent anions. To understand this process, loop dynamics were investigated using replica-exchange ... |
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Comparison of human optimized bacterial luciferase, firefly luciferase, and green fluorescent protein for continuous imaging of cell culture and animal models.
J. Biomed. Opt. 16(4) , 047003, (2011) Bioluminescent and fluorescent reporter systems have enabled the rapid and continued growth of the optical imaging field over the last two decades. Of particular interest has been noninvasive signal detection from mammalian tissues under both cell culture and... |