110448-31-2

110448-31-2 structure

Name: ML-9 Free Base
Chemical Name: 1-(5-chloronaphthalen-1-yl)sulfonyl-1,4-diazepane
CAS Number: 110448-31-2
Molecular Formula: C₁₅H₁₇ClN₂O₂S
Molecular Weight: 324.82600
Catalog: Signaling Pathways Cytoskeleton Myosin
Create Date: 2016-05-19 03:23:28
Modify Date: 2024-01-05 20:00:46
ML-9 (Free Base) is a selective and potent inhibitor of Akt kinase, inhibits myosin light-chain kinase (MLCK) and stromal interaction molecule 1 (STIM1) activity[3]. ML-9 (Free Base) inhibits inhibits MLCK, PKA and PKC activity with Ki values of 4, 32 and 54 μM, respectively[1]. ML-9 (Free Base) induces autophagy by stimulating autophagosome formation and inhibiting their degradation[3].

Name	1-(5-chloronaphthalen-1-yl)sulfonyl-1,4-diazepane
Synonyms	Lopac-C-1172 1H-1,4-Diazepine,1-[(5-chloro-1-naphthalenyl)sulfonyl]hexahydro Tocris-0431

Description	ML-9 (Free Base) is a selective and potent inhibitor of Akt kinase, inhibits myosin light-chain kinase (MLCK) and stromal interaction molecule 1 (STIM1) activity[3]. ML-9 (Free Base) inhibits inhibits MLCK, PKA and PKC activity with Ki values of 4, 32 and 54 μM, respectively[1]. ML-9 (Free Base) induces autophagy by stimulating autophagosome formation and inhibiting their degradation[3].
Related Catalog	Research Areas >> Cancer Signaling Pathways >> Cytoskeleton >> Myosin
In Vitro	ML9 (Free Base) (0-100 μM; 0-24 hours) has no reduction in cardiomyocyte viability, 50-100 μM significantly induces cell death[2]. ML9 (Free Base) (50 μM; 1-4 hours) significantly increases cleaved caspase-3 levels, decreased STIM1 protein levels by about 42%[2]. Cell Viability Assay[1] Cell Line: Neonatal rat ventricular myocytes (NRVM) cells Concentration: 0, 10, 50 and 100 μM Incubation Time: 0, 1, 4, 8 and 24 hours Result: Decreased cell viability at 50–100 μM concentration. Apoptosis Analysis[1] Cell Line: Neonatal rat ventricular myocytes (NRVM) cells Concentration: 50 μM Incubation Time: 0, 1, 4, 8 hours Result: Induced cardiomyocyte death through necrosis and apoptosis.
References	[1]. Ito S, et al. ML-9, a myosin light chain kinase inhibitor, reduces intracellular Ca2+ concentration in guinea pig trachealis.Eur J Pharmacol. 2004 Feb 23;486(3):325-33. [2]. Shaikh S, et al. The STIM1 inhibitor ML9 disrupts basal autophagy in cardiomyocytes by decreasing lysosome content.Toxicol In Vitro. 2018 Apr;48:121-127. [3]. Kondratskyi A1, et al.Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death.Cell Death Dis. 2014 Apr 24;5:e1193.

Molecular Formula	C₁₅H₁₇ClN₂O₂S
Molecular Weight	324.82600
Exact Mass	324.07000
PSA	57.79000
LogP	3.82470

Hazard Codes	Xi

110448-31-2	105637-50-1
1855860-24-0	300347-11-9
1267502-34-0	610323-32-5
2245072-20-0	81133-21-3
2247543-65-1	1194574-33-8
1158522-08-7	940364-43-2
1949815-97-7	2034579-71-8
134104-43-1	131615-57-1
6906-52-1	930439-75-1
1018584-77-4	1375289-11-4