623903-26-4
623903-26-4 structure
- Name: Tetrabromorhodamine 123 bromide
- Chemical Name: Tetrabromorhodamine 123 bromide
- CAS Number: 623903-26-4
- Molecular Formula: C21H13Br5N2O3
- Molecular Weight: 740.86
- Catalog: Research Areas Cancer
- Create Date: 2022-10-11 11:50:04
- Modify Date: 2024-01-10 11:01:15
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Tetrabromorhodamine 123 (TBR) bromide is a photosensitizer. Tetrabromorhodamine 123 bromide can be used for the research of photo dynamic therapy (PDT) and cancer[1].
| Name | Tetrabromorhodamine 123 bromide |
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| Description | Tetrabromorhodamine 123 (TBR) bromide is a photosensitizer. Tetrabromorhodamine 123 bromide can be used for the research of photo dynamic therapy (PDT) and cancer[1]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). A. Labeling of Cells: 1. Incubate the cells according to your normal protocol. 2. Cells are incubated with DMEM containing 5 μM TBR and maintained in the dark in a CO2 incubator at 37℃. 3. After 1 h, chang the medium to normal culture medium without phenol red, and cells were exposed to visible light with a 500 W Xe arc with a filter. B. The intracellular localization of TBR: 1. Cells are cultured in 35 mm diameter glassbottomed dishes for 48 h. Incubate the cells according to your normal protocol. 2. For triple-staining, medium is changed to a solution containing BODIPY-TR ceramide (5 μM). 3. Cells are incubated at 48℃ for 20 min and then further incubated at 37℃ for 1 h, after which the solution is changed to normal medium containing 5 μM TBR. 4. The stock solution (1 mM) of Hoechst 33342 was added to the medium (final concentration, 100 μM), and cells were incubated for 15 min. 5. Cells are washed with phosphate-buffered saline (plus Ca2+, Mg2+) and then observed with the aid of confocal laser scanning microscopy (LSM). |
| References |
| Molecular Formula | C21H13Br5N2O3 |
|---|---|
| Molecular Weight | 740.86 |