161180-12-7

161180-12-7 structure

Name: 7-hydroxycoumarinyl-γ-Linolenate
Chemical Name: (2-oxochromen-7-yl) octadeca-6,9,12-trienoate
CAS Number: 161180-12-7
Molecular Formula: C₂₇H₃₄O₄
Molecular Weight: 422.556
Catalog: Signaling Pathways Metabolic Enzyme/Protease Phospholipase
Create Date: 2017-09-25 18:23:49
Modify Date: 2024-01-14 18:50:53
7-Hydroxycoumarinyl-γ-linolenate is a cytosolic phospholipase A2 (cPLA2) fluorogenic substrate. 7-Hydroxycoumarinyl-γ-linolenate can be used to monitor the enzymatic activity of cPLA2[1].

Name	(2-oxochromen-7-yl) octadeca-6,9,12-trienoate
Synonyms	2-Oxo-2H-chromen-7-yl (6Z,9Z,12Z)-6,9,12-octadecatrienoate 6,9,12-Octadecatrienoic acid, 2-oxo-2H-1-benzopyran-7-yl ester, (6E,9E,12E)- 2-Oxo-2H-chromen-7-yl (6E,9E,12E)-6,9,12-octadecatrienoate 6,9,12-Octadecatrienoic acid, 2-oxo-2H-1-benzopyran-7-yl ester, (6Z,9Z,12Z)- 7-hydroxycoumarinyl--Linolenate

Description	7-Hydroxycoumarinyl-γ-linolenate is a cytosolic phospholipase A2 (cPLA2) fluorogenic substrate. 7-Hydroxycoumarinyl-γ-linolenate can be used to monitor the enzymatic activity of cPLA2[1].
Related Catalog	Signaling Pathways >> Metabolic Enzyme/Protease >> Phospholipase Research Areas >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). cPLA2α activity assay[1][2]: 1. Sonicate 200 μL of 1,2-O-tetradecyl-sn-glycero-3-phosphocholine (DTPC)/Triton X-100 high-affinity mixed micelle solution containing 960 μM Triton X-100 (HY-Y1883A), 60 μM DTPC, 50 μM 7-hydroxycoumarinyl-γ-linolenate, 50 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (pH = 7.2), 0.3 mM EDTA (HY-Y0682), 1 mM CaCl2 and 300 mM KCl. 2. Monitoring the hydrolysis of 7-hydroxycoumarinyl-γ-linolenate continuously with excitation at 360 nm and emission at 460 nm. 3. The formation of 7-hydroxycoumarin was monitored by fluorospectrometer.
References	[1]. Xiao Q, et al. Bufexamac ameliorates LPS-induced acute lung injury in mice by targeting LTA4H. Sci Rep. 2016 Apr 29;6:25298. [2]. Huang Z, et al. A continuous fluorescence-based assay for the human high-molecular-weight cytosolic phospholipase A2. Anal Biochem. 1994 Oct;222(1):110-5.