108477-18-5

108477-18-5 structure

Name: G5
Chemical Name: 3,5-bis-((Ξ)-4-nitro-benzylidene)-1,1-dioxo-tetrahydro-1λ6-thiopyran-4-one
CAS Number: 108477-18-5
Molecular Formula: C₁₉H₁₄N₂O₇S
Molecular Weight: 414.38900
Catalog: Signaling Pathways Apoptosis Apoptosis
Create Date: 2017-04-13 19:04:22
Modify Date: 2024-01-11 19:18:59
Ubiquitin Isopeptidase Inhibitor I, G5 (NSC 144303) is an apoptosome-independent caspase and apoptosis activator with IC50 values of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively.

Name	3,5-bis-((Ξ)-4-nitro-benzylidene)-1,1-dioxo-tetrahydro-1λ6-thiopyran-4-one
Synonyms	4h-thiopyran-4-one,tetrahydro-3,5-bis[(4-nitrophenyl)methylene]-1,1-dioxide ubiquitin isopeptidase inhibitor i, g5

Description	Ubiquitin Isopeptidase Inhibitor I, G5 (NSC 144303) is an apoptosome-independent caspase and apoptosis activator with IC50 values of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively.
Related Catalog	Signaling Pathways >> Apoptosis >> Apoptosis Research Areas >> Cancer
Target	IC50: 1.76 μM (E1A cells), 1.6 μM (E1A/C9DN cells)[1]
In Vitro	G5 is capable of activating an apoptosome-independent apoptotic pathway. It targets the ubiquitinproteasome system by inhibiting the ubiquitin isopeptidases. G5 induces a rather unique apoptotic pathway, which includes a Bcl-2-dependent but apoptosome-independent mitochondrial pathway with upregulation of the BH3-only protein Noxa, stabilization of theinhibitor of apoptosis antagonist Smac, but also the involvement of the death receptor pathway. G5 is a potent apoptotic inducer showing IC50s of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively[1].
Cell Assay	Different drug concentrations are used to determine the dose-response profile and to calculate the IC50 value. Cells are incubated with the required concentrations of the 57 compounds. After 24 or 48 hours of incubation, cell death is evaluated bytr ypan blue staining[1].
References	[1]. Aleo E, et al. Identification of new compounds that trigger apoptosome-independent caspase activation and apoptosis. Cancer Res. 2006 Sep 15;66(18):9235-44.