| In Vitro |
NUCC-390 (10 μM) produces strong (Ca)i response, but this effect can be blocked by the known potent and selective CXCR4 antagonist AMD3100[1]. NUCC-390 (10 μM) leads to increased levels of pERK, it has the capability of stimulating signaling activity downstream of CXCR4 receptors[1]. NUCC-390 exhibited the ability to induce CXCR4 receptor internalization, we assessed the cellular localization of YFP-tagged CXCR4 receptors expressed in HEK293 cells following treatment with SDF-1 or NUCC-390[1]. NUCC-390 (10 μM; 2 hours) can induce CXCR4 receptor internalization, and non--treated cells exhibits some diffuse expression of CXCR4-YFP throughout the cytosol and clear expression in the cell membrane in HEK cells[1]. Western Blot Analysis[1] Cell Line: C8161 cells Concentration: 10 μM Incubation Time: Result: Increased the level of pERK[1].
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