Rhodamine 800

Modify Date: 2024-01-04 12:15:53

Rhodamine 800 Structure
Rhodamine 800 structure
Common Name Rhodamine 800
CAS Number 137993-41-0 Molecular Weight 495.955
Density N/A Boiling Point N/A
Molecular Formula C26H26ClN3O5 Melting Point N/A
MSDS USA Flash Point N/A

 Use of Rhodamine 800


Rhodamine 800 is a near-infrared fluorescent dye.

 Names

Name Rhodamine 800
Synonym More Synonyms

 Rhodamine 800 Biological Activity

Description Rhodamine 800 is a near-infrared fluorescent dye.
Related Catalog
In Vitro Rhodamine 800 has different optical absorption spectra in various organic solvents and in aqueous solution in the visible and near-infrared regions. The absorption maximum at 683 nm in acetone shifts to 696 nm in DMSO without a significant change of the overall spectral profile. The fluorescence intensity of Rhodamine 800 varies markedly with the solvent, and decreases to less than one-fifth when the solvent is changed from acetone to water without a significant change of the emission peak[1].
Kinase Assay Rhodamine 800 (Rh800) and indocyanine green (ICG) are dissolved in PBS and their concentrations are set to 40 nM. To 3 mL of Rhodamine 800 solution (40 nM/PBS), 100 μL of p-sulfonatocalix[n]arenes (S[n]) (1 mM) is added. To this solution, microliter aliquots of acetylcholine (ACh) (350 mM) are added under stirring. After 2 min stirring, fluorescence spectra are measured. For the competitive fluorophore displacement by other neurotransmitters, amino acids, ammonium chloride, and choline, similar procedure as the above method is applied[2].
References

[1]. Sakanoue J, et al. Rhodamine 800 as a probe of energization of cells and tissues in the near-infrared region: a study with isolated rat liver mitochondria and hepatocytes. J Biochem. 1997 Jan;121(1):29-37.

[2]. Jin T. et al. Near-infrared fluorescence detection of acetylcholine in aqueous solution using a complex ofrhodamine 800 and p-sulfonatocalix[8]arene. Sensors (Basel). 2010;10(3):2438-49.

 Chemical & Physical Properties

Molecular Formula C26H26ClN3O5
Molecular Weight 495.955
Exact Mass 495.156097

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR NONH for all modes of transport

 Articles4

More Articles
Flow cytometry of DNA content using oxazine 750 or related laser dyes with 633 nm excitation.

Cytometry 7 , 107, (1986)

The laser dyes oxazine 750 (OX750), LD700, and rhodamine 800 (R800) can be used in an instrument employing a low-power helium-neon laser source for flow cytometry of DNA content in ethanol-fixed or de...

The effect of varying short-chain alkyl substitution on the molar absorptivity and quantum yield of cyanine dyes.

Anal. Chem. Insights 6 , 29-36, (2011)

The effect of varying short-chain alkyl substitution of the indole nitrogens on the spectroscopic properties of cyanine dyes was examined. Molar absorptivities and fluorescence quantum yields were det...

Near-infrared fluorescence detection of acetylcholine in aqueous solution using a complex of rhodamine 800 and p-sulfonatocalix[8]arene.

Sensors (Basel.) 10 , 2438-49, (2012)

The complexing properties of p-sulfonatocalix[n]arenes (n = 4: S[4], n = 6: S[6], and n = 8: S[8]) for rhodamine 800 (Rh800) and indocyanine green (ICG) were examined to develop a near-infrared (NIR) ...

 Synonyms

9-Cyano-2,3,6,7,12,13,16,17-octahydro-1H,5H,11H,15H-pyrido[3,2,1-ij]quinolizino[1',9':6,7,8]chromeno[2,3-f]quinolin-4-ium perchlorate