Rhodamine B

Modify Date: 2026-08-03 09:06:39

Rhodamine B Structure
Rhodamine B structure
Common Name Rhodamine B
CAS Number 81-88-9 Molecular Weight 479.010
Density 0.79 g/mL at 20 °C Boiling Point N/A
Molecular Formula C28H31ClN2O3 Melting Point 210-211 (dec.)(lit.)
MSDS Chinese USA Flash Point 12 °C
Symbol GHS05 GHS07
GHS05, GHS07
Signal Word Danger

 Use of Rhodamine B


Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and drugs.

 Names

This table lists Chinese names, IUPAC names and various aliases of this chemical substance.

Name rhodamine B
Synonym More Synonyms

 Rhodamine B Biological Activity

This table contains bioactivity, target information and biological‑assay experimental data of this compound.

Description Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and drugs.
Related Catalog
In Vitro Rhodamine B induces a concentration-dependent reduction of root meristem cells of A. cepa. mitotic activity. Rhodamine B induces various nuclear aberrations in A. cepa. root cells. In the 100 and 200 ppm rhodamine B groups, the frequencies of NBUDs and BN surpass those of the positive control (MMS) group. Rhodamine B-induced changes of H2O2 (a) and MDA (b) level increase in a concentration-dependent manner in A. cepa. roots[1].
References

[1]. Tan D, et al. Rhodamine B induces long nucleoplasmic bridges and other nuclear anomalies in Allium cepa root tip cells. Environ Sci Pollut Res Int. 2014 Mar;21(5):3363-70.

 Chemical & Physical Properties

This table shows physicochemical parameters including melting point, boiling point, density, solubility and optical rotation. Missing data is marked as N/A.

Density 0.79 g/mL at 20 °C
Melting Point 210-211 (dec.)(lit.)
Molecular Formula C28H31ClN2O3
Molecular Weight 479.010
Flash Point 12 °C
Exact Mass 478.202332
PSA 56.69000
LogP 2.56500
InChIKey PYWVYCXTNDRMGF-UHFFFAOYSA-N
SMILES CCN(CC)c1ccc2c(-c3ccccc3C(=O)O)c3ccc(=[N+](CC)CC)cc-3oc2c1.[Cl-]
Storage condition Store at RT.
Water Solubility SOLUBLE

 MSDS

This table provides MSDS information including hazard classification, first‑aid, fire‑fighting, spill handling, operation and storage instructions.

 Toxicological Information

This table summarizes toxicological test data, acute & chronic toxicity and ecological hazard parameters for this chemical.

CHEMICAL IDENTIFICATION

RTECS NUMBER :
BP3675000
CHEMICAL NAME :
Ammonium, (9-(o-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-yl idene)diethyl-, chloride
CAS REGISTRY NUMBER :
81-88-9
LAST UPDATED :
199710
DATA ITEMS CITED :
29
MOLECULAR FORMULA :
C28-H31-N2-O3.Cl
MOLECULAR WEIGHT :
479.06
WISWESSER LINE NOTATION :
T C666 BO EYJ EUK2&2 IR BVQ& MN2&2 &Q &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
500 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
112 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
89 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
887 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
144 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
180 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
50400 mg/kg/12W-C
TOXIC EFFECTS :
Liver - changes in liver weight Kidney, Ureter, Bladder - changes in bladder weight Nutritional and Gross Metabolic - weight loss or decreased weight gain
TYPE OF TEST :
TCLo - Lowest published toxic concentration
ROUTE OF EXPOSURE :
Inhalation
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
110 mg/kg/13W-I
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - structural or functional change in trachea or bronchi Nutritional and Gross Metabolic - weight loss or decreased weight gain Related to Chronic Data - death
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
3600 mg/kg/68W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria Blood - lymphoma, including Hodgkin's disease Tumorigenic - tumors at site of application
TYPE OF TEST :
TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
3870 mg/kg/68W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria Tumorigenic - tumors at site of application
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Intraperitoneal
DOSE :
60 mg/kg
SEX/DURATION :
female 7-10 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus)
TYPE OF TEST :
Sex chromosome loss and nondisjunction

MUTATION DATA

TYPE OF TEST :
Cytogenetic analysis
TEST SYSTEM :
Mammal - species unspecified Fibroblast
DOSE/DURATION :
2 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 88,211,1981 *** REVIEWS *** IARC Cancer Review:Animal Limited Evidence IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972- Volume(issue)/page/year: 16,221,1978 IARC Cancer Review:Human No Adequate Data IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972- Volume(issue)/page/year: 16,221,1978 IARC Cancer Review:Group 3 IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year: 7,56,1987 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 84376 No. of Facilities: 3759 (estimated) No. of Industries: 38 No. of Occupations: 37 No. of Employees: 40674 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 84376 No. of Facilities: 12726 (estimated) No. of Industries: 91 No. of Occupations: 88 No. of Employees: 198323 (estimated) No. of Female Employees: 75440 (estimated)

 Safety Information

This table covers safety warnings, protective measures, incompatible substances and disposal guidelines for this compound.

Symbol GHS05 GHS07
GHS05, GHS07
Signal Word Danger
Hazard Statements H302-H318-H412
Precautionary Statements P273-P280-P305 + P351 + P338
Personal Protective Equipment dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes Xn:Harmful
Risk Phrases R22;R41;R68
Safety Phrases S7-S16-S24/25-S26-S36/37/39-S39
RIDADR UN 1219 3/PG 2
WGK Germany 2
RTECS BP3675000
Hazard Class 3.0
HS Code 32041300

 Synthetic Route

This table presents publicly reported synthetic routes, reaction conditions, reactants and product information of the compound.

~%

Rhodamine B Structure

Rhodamine B

CAS#:81-88-9

Learn More
Literature: Dyes and Pigments, , vol. 98, # 3 p. 339 - 346

~%

Rhodamine B Structure

Rhodamine B

CAS#:81-88-9

Learn More
Literature: Dyes and Pigments, , vol. 98, # 3 p. 339 - 346

~%

Rhodamine B Structure

Rhodamine B

CAS#:81-88-9

Learn More
Literature: Bioorganic and Medicinal Chemistry Letters, , vol. 23, # 10 p. 2916 - 2919

 Customs

This table shows customs‑related data including HS‑code, tariff and regulatory conditions for import and export.

HS Code 2922299090
Summary 2922299090. other amino-naphthols and other amino-phenols, other than those containing more than one kind of oxygen function, their ethers and esters; salts thereof. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:30.0%

 Articles211

More Articles

This table lists relevant public references with title, journal and publication metadata for this compound.

The dynamics of giant unilamellar vesicle oxidation probed by morphological transitions.

Biochim. Biophys. Acta 1838(10) , 2615-24, (2014)

We have studied the dynamics of Lissamine Rhodamine B dye sensitization-induced oxidation of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) giant unilamellar vesicles (GUVs), where the progression of...

Graphene oxide - gelatin nanohybrids as functional tools for enhanced Carboplatin activity in neuroblastoma cells.

Pharm. Res. 32 , 2132-43, (2015)

Preparation of Nanographene oxide (NGO) - Gelatin hybrids for efficient treatment of Neuroblastoma.Nanohybrids were prepared via non-covalent interactions. Spectroscopic tools have been used to discri...

Facile room-temperature synthesis of carboxylated graphene oxide-copper sulfide nanocomposite with high photodegradation and disinfection activities under solar light irradiation.

Sci. Rep. 5 , 16369, (2015)

Carboxylic acid functionalized graphene oxide-copper (II) sulfide nanoparticle composite (GO-COOH-CuS) was prepared from carboxylated graphene oxide and copper precursor in dimethyl sulfoxide (DMSO) b...

 Rhodamine BBioassay

View more

This table contains target information, in‑vitro & in‑vivo assay data for this compound.

Name: p53 small molecule agonists, cell-based qHTS assay
Source: 824
External Id: P53344
Name: p53 small molecule agonists, cell-based qHTS assay with human liver microsomes
Source: 824
Target: tumor suppressor p53 [Homo sapiens]
External Id: P53MS233
Name: p53 small molecule agonists, cell-based qHTS assay with rat liver microsomes
Source: 824
Target: tumor suppressor p53 [Homo sapiens]
External Id: P53MS898
Total 218, Current Page 1 of 22
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 Synonyms

This table collects all English aliases, systematic names and CAS‑related synonyms of the compound.

Basic Violet 10
redno213
D and C Red No. 19
c.i.749
9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride
MFCD00011931
AKA214
rhodamine blue
AKA213
Pilot578
EINECS 201-383-9
Rhodamine B
rhodamine hydrochloride
C.I. Basic Violet 10

 Rhodamine B | FAQ

This section contains frequently‑asked‑questions about this compound, including basic parameters, properties, storage conditions and corresponding answers.

Q: What is the melting point of rhodamine B?
A: Melting point of rhodamine B is 210-211 (dec.)(lit.).
Q: What English synonyms does rhodamine B have?
A: English synonyms of rhodamine B: Basic Violet 10, redno213, D and C Red No. 19, c.i.749, 9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride, MFCD00011931, AKA214, rhodamine blue, AKA213, Pilot578, EINECS 201-383-9, Rhodamine B, rhodamine hydrochloride, C.I. Basic Violet 10.
Q: What is the molecular formula of rhodamine B?
A: Molecular formula of rhodamine B is C28H31ClN2O3.
Q: What are the downstream products of rhodamine B?
A: Related downstream products(CAS) of rhodamine B: 39393-39-0;509-34-2;103-82-2;79-14-1;849585-22-4;57-55-6;626-89-1;100-21-0.
Q: What are the uses of rhodamine B?
A: Main uses of rhodamine B: Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and drugs.
Q: What is the InChIKey of rhodamine B?
A: InChIKey of rhodamine B is PYWVYCXTNDRMGF-UHFFFAOYSA-N.
Q: What are the upstream materials of rhodamine B?
A: Related upstream materials(CAS) of rhodamine B: 74317-53-6.
Q: How is the water solubility of rhodamine B?
A: Water solubility of rhodamine B: SOLUBLE.
Q: What is the CAS number of rhodamine B?
A: CAS number of rhodamine B is 81-88-9.
Q: What is the LogP of rhodamine B?
A: LogP of rhodamine B is 2.56500.

 Rhodamine Bfactory

This table lists manufacturers and suppliers of this compound, including vendor names and product supply reference information.

Shanghai Nianxing Industrial Co., Ltd
BioBioPha
Dayang Chem (Hangzhou) Co., Ltd.
Henan Tianfu Chemical Co., Ltd.
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