| Description |
SR1555 is a specific retinoic acid receptor-related orphan nuclear receptor γ (RORγ) inverse agonist with an IC50 value of 1 μM. SR1555 not only inhibits TH17 cell development and function but also increases the frequency of T regulatory cells, as well as inhibits the expression of IL-17. SR1555 can be used for researching autoimmune diseases[1].
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| Related Catalog |
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| Target |
RORγ:1 μM (IC50)
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| In Vitro |
SR1555 (10 μM; 24 h) inhibits Il17a gene expression by greater than 70%in EL4 cells[1]. SR1555 (10 μM; 24 h) inhibits IL-17 protein expression, inhibits TH17 cell differentiation and function, and does not induce cell death during TH17 cell differentiation[1]. SR1555 (10 μM; 24 h) effectively increases the gene expression of Foxp3 while simultaneously suppressing the gene expression of Rorγt[1]. SR1555 increases the frequency of T regulatory cells as evidenced by the almost 2-fold increase in the expression of Foxp3+ T cells in the splenocyte culture[1]. RT-PCR[1] Cell Line: EL4 cells Concentration: 10 μM Incubation Time: 24 h Result: Inhibited Il17a gene expression by greater than 70%. Cell Differentiation Assay[1] Cell Line: Splenocytes (cultured under TH17 polarizing conditions for 3 days) Concentration: 10 μM Incubation Time: 24 h Result: Inhibited IL-17 protein expression, inhibited TH17 cell differentiation and function, and did not induce cell death during TH17 cell differentiation. RT-PCR[1] Cell Line: Splenocytes (cultured under T regulatory cell polarizing conditions for 3 days) Concentration: 10 μM Incubation Time: 24 h Result: Effectively increased the gene expression of Foxp3 while simultaneously suppressing the gene expression of Rorγt.
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| References |
[1]. Solt LA, Kumar N, He Y, Kamenecka TM, Griffin PR, Burris TP. Identification of a selective RORγ ligand that suppresses T(H)17 cells and stimulates T regulatory cells. ACS Chem Biol. 2012 Sep 21;7(9):1515-9.
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