Uniblue A sodium salt

Uniblue A sodium salt structure	Common Name	Uniblue A sodium salt
CAS Number	14541-90-3	Molecular Weight	506.48300
Density	N/A	Boiling Point	N/A
Molecular Formula	C₂₂H₁₅N₂NaO₇S₂	Melting Point	>300ºC(lit.)
MSDS	Chinese USA	Flash Point	N/A
Symbol	GHS07	Signal Word	Warning

Name	Uniblue A sodium salt
Synonym	More Synonyms

Description	Uniblue A sodium is a reactive protein stain that can be used in the covalent pre-gel staining of the protein (Ex=594 nM)[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. Prepare the derivatization buffer: 100 mM NaHCO3, 10% SDS, pH 8-9. 2. Add 10 µL of 200 mM Uniblue A solution (dissolved in derivatization buffer) to 90 µL protein solution. 3. The samples are heated at 100°C for 1 min to perform the staining. 4. Add 100 µL of reducing solution composed of 10% glycerol and 20 mM dithiotreitol (DTT) in 200 mM Tris buffer with pH 6.8. 5. Excess Uniblue A reacts with Tris, resulting in a blue compound, serving as running front indicator in electrophoresis. 6. The sample is heated another minute at 100°C in order to achieve efficient reduction and allowed to cool to room temperature. 7. Add 20 µL alkylation solution containing 550 mM iodoacetamide (IAA), incubated for 5 minutes. 8. the samples are subjected to SDS-PAGE. Note: Dry protein samples or samples in compatible buffers (i.e. free of amines) can be diluted directly with the derivatization buffer solution to 5 mg/mL. In other cases, a prior trichloroacetic acid (TCA)/acetone precipitation or buffer exchange by ultrafiltration is recommended (see below). As positive control, bovine serum albumin (BSA) in derivatization buffer is used at a concentration of 10 mg/mL.
References	[1]. Marco A Mata-Gómez, et al. Accelerated identification of proteins by mass spectrometry by employing covalent pre-gel staining with Uniblue A. PLoS One. 2012;7(2):e31438.

Melting Point	>300ºC(lit.)
Molecular Formula	C₂₂H₁₅N₂NaO₇S₂
Molecular Weight	506.48300
Exact Mass	506.02200
PSA	180.29000
LogP	5.42490

Name:	Uniblue A Sodium Salt 98% (UV-VIS) Material Safety Data Sheet
Synonym:	None Known
CAS:	14541-90-3

Section 1 - Chemical Product MSDS Name:Uniblue A Sodium Salt 98% (UV-VIS) Material Safety Data Sheet
Synonym:None Known

Section 2 - COMPOSITION, INFORMATION ON INGREDIENTS

CAS#	Chemical Name	content	EINECS#
14541-90-3	Uniblue A Sodium Salt, (UV-VIS)	98	238-573-6

Hazard Symbols: None Listed.
Risk Phrases: None Listed.

Section 3 - HAZARDS IDENTIFICATION

EMERGENCY OVERVIEW
The toxicological properties of this material have not been fully investigated.
Potential Health Effects
Eye:
May cause eye irritation.
Skin:
May cause skin irritation.
Ingestion:
May cause irritation of the digestive tract. The toxicological properties of this substance have not been fully investigated.
Inhalation:
May cause respiratory tract irritation. The toxicological properties of this substance have not been fully investigated.
Chronic:
No information found.

Section 4 - FIRST AID MEASURES
Eyes: Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.
Skin:
Get medical aid. Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse.
Ingestion:
Never give anything by mouth to an unconscious person. Get medical aid. Do NOT induce vomiting. If conscious and alert, rinse mouth and drink 2-4 cupfuls of milk or water.
Inhalation:
Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
Notes to Physician:

Section 5 - FIRE FIGHTING MEASURES
General Information:
As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Runoff from fire control or dilution water may cause pollution.
Extinguishing Media:
Use agent most appropriate to extinguish fire.

Section 6 - ACCIDENTAL RELEASE MEASURES
General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks:
Vacuum or sweep up material and place into a suitable disposal container. Clean up spills immediately, observing precautions in the Protective Equipment section. Avoid generating dusty conditions.
Provide ventilation.

Section 7 - HANDLING and STORAGE
Handling:
Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation.
Storage:
Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.

Section 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION
Engineering Controls:
Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use process enclosure, local exhaust ventilation, or other engineering controls to control airborne levels.
Exposure Limits CAS# 14541-90-3: Personal Protective Equipment Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin:
Wear appropriate protective gloves to prevent skin exposure.
Clothing:
Wear appropriate protective clothing to minimize contact with skin.
Respirators:
Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - PHYSICAL AND CHEMICAL PROPERTIES

Physical State: Powder
Color: dark blue
Odor: Not available.
pH: Not available.
Vapor Pressure: Not available.
Viscosity: Not available.
Boiling Point: Not available.
Freezing/Melting Point: Not available.
Autoignition Temperature: Not available.
Flash Point: Not available.
Explosion Limits, lower: Not available.
Explosion Limits, upper: Not available.
Decomposition Temperature:
Solubility in water:
Specific Gravity/Density:
Molecular Formula: C22H15N2NaO7S2
Molecular Weight: 506.48

Section 10 - STABILITY AND REACTIVITY
Chemical Stability:
Stable under normal temperatures and pressures.
Conditions to Avoid:
Incompatible materials, dust generation, excess heat, strong oxidants.
Incompatibilities with Other Materials:
Oxidizing agents.
Hazardous Decomposition Products:
Nitrogen oxides, carbon monoxide, irritating and toxic fumes and gases, carbon dioxide, sodium oxide, sulfur oxides (SOx), including sulfur oxide and sulfur dioxide.
Hazardous Polymerization: Has not been reported

Section 11 - TOXICOLOGICAL INFORMATION
RTECS#:
CAS# 14541-90-3 unlisted.
LD50/LC50:
Not available.
Carcinogenicity:
Uniblue A Sodium Salt, (UV-VIS) - Not listed by ACGIH, IARC, or NTP.

Section 12 - ECOLOGICAL INFORMATION

Section 13 - DISPOSAL CONSIDERATIONS
Dispose of in a manner consistent with federal, state, and local regulations.

Section 14 - TRANSPORT INFORMATION

IATA
Shipping Name: Not regulated.
Hazard Class:
UN Number:
Packing Group:
IMO
Shipping Name: Not regulated.
Hazard Class:
UN Number:
Packing Group:
RID/ADR
Shipping Name: Not regulated.
Hazard Class:
UN Number:
Packing group:

Section 15 - REGULATORY INFORMATION

European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols: Not available.
Risk Phrases:
Safety Phrases:
S 24/25 Avoid contact with skin and eyes.
WGK (Water Danger/Protection)
CAS# 14541-90-3: No information available.
Canada
CAS# 14541-90-3 is listed on Canada's DSL List.
CAS# 14541-90-3 is not listed on Canada's Ingredient Disclosure List.
US FEDERAL
TSCA
CAS# 14541-90-3 is listed on the TSCA inventory.

SECTION 16 - ADDITIONAL INFORMATION
N/A

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H302
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xn: Harmful;
Risk Phrases	R22
RIDADR	NONH for all modes of transport

Lysine-directed staining of proteins for MS-based analyses.

Electrophoresis 34(3) , 401-4, (2013)

Visualization of proteins and MS-based analyses are elemental tasks in modern biochemistry. Nevertheless, reports about covalent protein dyes and their suitability for subsequent MS experiments remain...

Catalytic decomposition of the reactive dye Uniblue A on hematite. Modeling of the reactive surface.

Water Res. 35(3) , 750-60, (2001)

Experimental results from the adsorption and subsequent catalytic combustion of the reactive dye Uniblue A on hematite indicate that this iron oxide can be used as an affordable catalyst for environme...

Interaction of anthraquinone dyes with lysozyme: evidences from spectroscopic and docking studies.

J. Hazard. Mater. 175(1-3) , 985-91, (2010)

The interaction between lysozyme and anthraquinone dyes such as Alizarin Red S, Acid blue 129 and Uniblue was studied using steady state, time resolved fluorescence measurements and docking studies. A...

EINECS 238-573-6

Acid Blue 215

UNIBLUE A SODIUM SALT

MFCD00010794

Uniblue A sodium salt

Use of Uniblue A sodium salt

Names

Uniblue A sodium salt Biological Activity

Chemical & Physical Properties

MSDS

Safety Information

Articles7

Synonyms