| In Vitro |
Wistin, is a PPARγ agonist that can induce the mRNA expression of the PPARγ target gene adiponectin in mouse 3T3-L1 cells[2]. Wistin inhibits cellular triglyceride accumulation in hepatocytes (P<0.05 at 10 μg/mL) in a dose-dependent manner[2]. Wistin (10 μg/mL) results in a 2.5-, 7.2-, and 14.8-fold increase in the expression levels of CPT1a, ACO, and ACS, respectively[2]. Treatment with Wistin for 48 h significantly decreases the amount of cellular TG in mouse primary hepatocytes in a dose-dependent manner[2]. Treatment with wistin enhanced the marker of adipocyte differentiation, such as triglyceride accumulation in 3T3-L1 cells[3]. RT-PCR[2] Cell Line: Mouse hepatocytes. Concentration: 1 or 10 μg/mL. Incubation Time: 48 h. Result: Significantly increased PPARα mRNA levels in a dose-dependent manner.
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