4-Nitrophenyl phosphate disodium hexahydrate

Modify Date: 2024-01-05 21:26:55

4-Nitrophenyl phosphate disodium hexahydrate Structure
4-Nitrophenyl phosphate disodium hexahydrate structure
Common Name 4-Nitrophenyl phosphate disodium hexahydrate
CAS Number 333338-18-4 Molecular Weight 371.14400
Density N/A Boiling Point 457.8ºC at 760mmHg
Molecular Formula C6H16NNa2O12P Melting Point >300ºC(lit.)
MSDS Chinese USA Flash Point 230.7ºC

 Use of 4-Nitrophenyl phosphate disodium hexahydrate


4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].

 Names

Name disodium,(4-nitrophenyl) phosphate,hexahydrate
Synonym More Synonyms

  Biological Activity

Description 4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].
Related Catalog
In Vitro 4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE)[2].
References

[1]. Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015;71(Pt 2):199-205.

[2]. Qi S, et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020;145(11):3871-3877.

 Chemical & Physical Properties

Boiling Point 457.8ºC at 760mmHg
Melting Point >300ºC(lit.)
Molecular Formula C6H16NNa2O12P
Molecular Weight 371.14400
Flash Point 230.7ºC
Exact Mass 371.02100
PSA 183.43000
LogP 2.08010
Appearance of Characters tablets | white to pale yellow
Storage condition 2-8°C
Water Solubility H2O: 25 mg/mL at 20 °C, clear, yellow-green

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases 24/25
RIDADR NONH for all modes of transport
WGK Germany 3

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 Synonyms

MFCD00066288
di-Sodium 4-nitrophenyl phosphate
pNPP disodium salt hexahydrate
DisodiuM 4-Nitrophenylphosphate Hexahydrate
Phosphoric Acid 4-Nitrophenyl Ester Disodium Salt Hexahydrate
Phosphatase substrate
4-Nitrophenyl Phosphate,Disodium Salt Hexahydrate
p-Nitrophenyl phosphate disodium hexahydrate
pNPP disodium hexahydrate
4-Nitrophenyl phosphate disodium salt hexahydrate
p-Nitrophenyl phosphate disodium hexahydrate,Disodium 4-nitrophenyl phosphate hexahydrate,di
Sodium 4-nitrophenyl phosphate hexahydrate
EINECS 224-246-5
Disodium 4-Nitrophenyl Phosphate Hexahydrate