| In Vitro |
Norartocarpetin has strong mushroom tyrosinase inhibitory activity with an IC50 value of 0.47 μM[1]. Norartocarpetin (0-100 μM; 48 h) leds to dose-dependent cytotoxic effects in NCI-H460 cells and MRC-9 cells with IC50 values of 22 μM and 85 μM, respectively[2]. Norartocarpetin (0, 11, 22, and 44 μM; 24 h) led to blockade of Ras/Raf/MAPK signalling pathway[2]. Norartocarpetin (0, 11, 22, and 44 μM, 24 h) induced the apoptosis of the human lung carcinoma cells (NCI-H460)[2]. Norartocarpetin (0, 11, 22, and 44 μM) leds to S-phase cell cycle arrest[2]. Norartocarpetin (22 μM; 24 h) leds to dose-dependent suppression of cell invasion[2]. Norartocarpetin (0, 11, 22, and 44 μM, 24 h) leds to significant inhibition of cell migration[2]. Cell Viability Assay[2] Cell Line: MRC-9 (normal fibroblast lung cells) and NCI-H460 (human lung cancer cells) Concentration: 0, 3.12, 6.25, 12.5, 25, 50, and 100 μM (first dissolved in DMSO at 1.0 mg/mL and then diluted to different concentrations using DMSO, 30 μL, 20min) Incubation Time: 48 h Result: Repressed the viability of NCI-H460 cells in a dose-dependent manner. Western Blot Analysis[2] Cell Line: MRC-9 and NCI-H460 cells Concentration: 0, 11, 22, and 44 μM Incubation Time: 24 h Result: Significantly reduced the expression of p-RAS, p-RAF and p-P38 and unchanged the expressions of RAS, RAF and P38. Apoptosis Analysis[2] Cell Line: MRC-9 and NCI-H460 cells Concentration: 0. 11, 22 and 44 μM Incubation Time: 24 h Result: Led to dose-dependent apoptosis and induced DNA damage. Cell Cycle Analysis[2] Cell Line: MRC-9 and NCI-H460 cells Concentration: 0, 11, 22, and 44 μM Incubation Time: Result: Showed increase of S-phase cells and the percentage of S-phase NCI-H460 LC cells was 30%, 40%, 55% and 70% at 0, 11, 22 and 44 μM concentrations, respectively. Cell Invasion AssayMRC-9 and NCI-H460 cells Cell Line: MRC-9 and NCI-H460 cells Concentration: 22 μM Incubation Time: 24 h Result: Significantly reducted (from 100% to about 40%) in the invasion of NCI-H460 human LC cells. Cell Migration Assay [2] Cell Line: MRC-9 and NCI-H460 cells Concentration: 0, 11, 22, and 44 μM Incubation Time: 24 h Result: Decreased remarkably the cell invasion in NCI-H460 LC cells in a concentration- dependent manner.
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