Tetramethylbenzidine

Modify Date: 2024-01-02 01:17:33

Tetramethylbenzidine structure	Common Name	Tetramethylbenzidine
	CAS Number	54827-17-7	Molecular Weight	240.343
	Density	1.1±0.1 g/cm3	Boiling Point	368.6±37.0 °C at 760 mmHg
	Molecular Formula	C₁₆H₂₀N₂	Melting Point	168-171 °C(lit.)
	MSDS	USA	Flash Point	210.8±26.0 °C

Use of Tetramethylbenzidine

3,3’,5,5’-Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

Name	Tetramethylbenzidine
Synonym	More Synonyms

Description	3,3’,5,5’-Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others

Density	1.1±0.1 g/cm3
Boiling Point	368.6±37.0 °C at 760 mmHg
Melting Point	168-171 °C(lit.)
Molecular Formula	C₁₆H₂₀N₂
Molecular Weight	240.343
Flash Point	210.8±26.0 °C
Exact Mass	240.162643
PSA	52.04000
LogP	3.40
Vapour Pressure	0.0±0.8 mmHg at 25°C
Index of Refraction	1.618
Storage condition	2-8°C
Stability	Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents.
Water Solubility	Slightly soluble.

Tetramethylbenzidine MSDS(Chinese)

CHEMICAL IDENTIFICATION

RTECS NUMBER :: DV2300000

CHEMICAL NAME :: (1,1'-Biphenyl)-4,4-diamine, 3,3',5,5'-tetramethyl-

CAS REGISTRY NUMBER :: 54827-17-7

LAST UPDATED :: 199710

DATA ITEMS CITED :: 11

MOLECULAR FORMULA :: C16-H20-N2

MOLECULAR WEIGHT :: 240.38

WISWESSER LINE NOTATION :: ZR B1 F1 DR DZ C1 E1

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 135 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Bird - quail

DOSE/DURATION :: >316 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: Micronucleus test

MUTATION DATA

TYPE OF TEST :: Mutation in mammalian somatic cells

TEST SYSTEM :: Rodent - mouse Lymphocyte

DOSE/DURATION :: 30700 ug/L

REFERENCE :: EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year: 12(Suppl 13),37,1988

Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Faceshields;Gloves
Hazard Codes	Xi:Irritant
Risk Phrases	R36/37/38
Safety Phrases	S26-S36-S36/37
RIDADR	UN 2796 8/PG 2
WGK Germany	3
RTECS	DV2300000
Hazard Class	8.0
HS Code	29215990

4-CHLORO-2,6-DI...

CAS#:24596-18-7

~93%

Tetramethylbenzidine

CAS#:54827-17-7

Literature: Cheng, Jinsheng; Zhang, Gencheng; Du, Jin; Tang, Longhua; Xu, Jingying; Li, Jinghong Journal of Materials Chemistry, 2011 , vol. 21, # 10 p. 3485 - 3494

4-bromo-2,6-xylidine

CAS#:24596-19-8

~65%

Tetramethylbenzidine

CAS#:54827-17-7

2,6-xylidine

CAS#:87-62-7

Literature: Imperial Chemical Industries Limited Patent: US4022795 A1, 1977 ;

N/A

CAS#:80387-68-4

Tetramethylbenzidine

CAS#:54827-17-7

N/A

CAS#:80387-72-0

2,6-xylidine

CAS#:87-62-7

Literature: Journal of Organic Chemistry, , vol. 47, # 9 p. 1691 - 1696

2,6-dimethyl ph...

CAS#:603-77-0

Tetramethylbenzidine

CAS#:54827-17-7

Literature: Journal of Organic Chemistry, , vol. 47, # 9 p. 1691 - 1696

Precursor 3
CAS#:24596-18-7 4-CHLORO-2,6-DI... CAS#:24596-19-8 4-bromo-2,6-xylidine CAS#:603-77-0 2,6-dimethyl ph...
DownStream 1
CAS#:144653-01-0 2-bromo-5-(4-br...

HS Code	2921590090
Summary	2921590090. other aromatic polyamines and their derivatives; salts thereof. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:30.0%

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3355TMB

3,3,5',5'-Tetramethylbenzidine

UNII-3B3T5CB8EO

TMB/H

TMB-E

TMB/M

3,3’,5,5’-tetramethylbenzidine

EINECS 259-364-6

TMB

BM BLUE

3,3',5,5'-Tetramethyl-4,4'-biphenyldiamine

3,3',5,5'-tetramethyl-4,4'-diamino-1,1'-biphenyl

TMB QUICK

3,3',5,5'-tetramethylbenzidine

Tmbz

MFCD00007748

3,3',5,5'-Tetramethylbiphenyl-4,4'-diamine

3,5,3',5'-tetramethylbenzidine

TMB EASY

TMB-B

[1,1'-Biphenyl]-4,4'-diamine, 3,3',5,5'-tetramethyl-

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China
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China
Product Name: 3,3',5,5'-Tetramethylbenzidine
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