Fluorescein Diacetate

Modify Date: 2024-01-02 13:04:53

Fluorescein Diacetate structure	Common Name	Fluorescein Diacetate
	CAS Number	596-09-8	Molecular Weight	416.380
	Density	1.5±0.1 g/cm3	Boiling Point	604.7±55.0 °C at 760 mmHg
	Molecular Formula	C₂₄H₁₆O₇	Melting Point	200-203 °C(lit.)
	MSDS	Chinese USA	Flash Point	264.0±31.5 °C

Use of Fluorescein Diacetate

Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.

Name	Fluorescein Diacetate
Synonym	More Synonyms

Description	Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.
Related Catalog	Signaling Pathways >> Others >> Others Biochemical Assay Reagents Research Areas >> Others
In Vitro	Fluorescein diacetate (FDA) is an acetylated derivative of the green fluorescent dye fluorescein[1]. Fluorescein diacetate (FDA), a fluorescent probe used for vital staining, is a fluorescently activated by esterolytic activity of human Pi-class glutathione S-transferase (hGSTP1) selectively among various cytosolic GSTs. Fluorescence activation of Fluorescein diacetate susceptible to GST inhibitors is observed in MCF7 cells exogenously overexpressing hGSTP1, but not in cells overexpressing hGSTA1 or hGSTM1. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1. To investigate whether the fluorescence activation is due to hGSTP1 activity, Fluorescein diacetate is incubated with recombinant hGSTP1-1 and GSH in vitro. Remarkable fluorescence activation is observed in the presence of both hGSTP1-1 and GSH, whereas only slight activation is observed in the absence of either of them or when the enzyme is heat inactivated. This suggests that the fluorescence activation of Fluorescein diacetate depends on hGSTP1-1 activity. From the linear relationship between the rate of increase in fluorescence and the hGSTP1-1 concentration, the specific activity of the enzyme for 1 μM Fluorescein diacetate is determined to be 79±15 nmol/min/mg protein. Fluorescein diacetate is applicable as a fluorogenic substrate for evaluating inhibitors of GSTP1-1 in vitro. For Fluorescein diacetate as a substrate, both Ethacrynic acid (EA) and NBDHEX suppress the hGSTP1-1-dependent fluorescent increase in a concentration-dependent manner, with IC50s of 3.3±0.5 μM and 0.61±0.04 μM, respectively[2].
Cell Assay	MCF7 cells (2-3×105) are seeded in a 35-mm glass bottom dish before the experiment. Prior to imaging, cells are washed with 1 mL PBS, then incubated in 1 mL Hanks’ Balanced Salt solution (HBSS(+) without phenol red) containing 1 μM Fluorescein diacetate (0.1% DMSO as a cosolvent) for 5 min at 37°C. Cells are washed twice with 1 mL PBS and 1 mL HBSS is added before imaging. Fluorescence images of fluorescein and DsRed-Express2 were acquired in the FITC channel (excitation at 473 nm) and the DsRed channel (excitation 559 nm). The 16-bit images obtained are analysed[2].
References	[1]. Boyd V, et al. Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell PermeableNucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84. [2]. Fujikawa Y, et al. Fluorescein diacetate (FDA) and its analogue as substrates for Pi-class glutathione S-transferase (GSTP1) and their biological application. Talanta. 2018 Mar 1;179:845-852.

Density	1.5±0.1 g/cm3
Boiling Point	604.7±55.0 °C at 760 mmHg
Melting Point	200-203 °C(lit.)
Molecular Formula	C₂₄H₁₆O₇
Molecular Weight	416.380
Flash Point	264.0±31.5 °C
Exact Mass	416.089600
PSA	88.13000
LogP	2.77
Vapour Pressure	0.0±1.7 mmHg at 25°C
Index of Refraction	1.681
Storage condition	−20°C

Fluorescein Diacetate MSDS(Chinese)

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xi
Risk Phrases	R36/37/38
Safety Phrases	S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3

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Precursor 9
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DownStream 1
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