Indole-3-carbinol

Modify Date: 2025-08-20 13:47:26

Indole-3-carbinol structure	Common Name	Indole-3-carbinol
	CAS Number	700-06-1	Molecular Weight	147.174
	Density	1.3±0.1 g/cm3	Boiling Point	360.6±17.0 °C at 760 mmHg
	Molecular Formula	C₉H₉NO	Melting Point	96-99 °C(lit.)
	MSDS	Chinese USA	Flash Point	171.9±20.9 °C
	Symbol	GHS07	Signal Word	Warning

Use of Indole-3-carbinol

Indole-3-carbinol suppresses NF-κB activity and also is an Aryl hydrocarbon receptor (AhR) agonist.

Name	indole-3-methanol
Synonym	More Synonyms

Description	Indole-3-carbinol suppresses NF-κB activity and also is an Aryl hydrocarbon receptor (AhR) agonist.
Related Catalog	Signaling Pathways >> Immunology/Inflammation >> Aryl Hydrocarbon Receptor Natural Products >> Alkaloid Research Areas >> Cancer
Target	NF-κB AhR
In Vitro	It is found that Indole-3-carbinol (I3C) inhibits the proliferation of THP-1 cells in a dose- and time dependent manner with minimal toxicity over normal monocytes. The AhR target genes (CYP1A1, IL1β) are overexpressed upon Indole-3-carbinol treatment (p<0.05 to p<0.001). The antiproliferative effects of Indole-3-carbinol are in association with programing cell death. Indole-3-carbinol downregulates BCL2 and upregulates FasR in THP-1 cells (p<0.05 to p<0.001). G1 cell cycle arrest is also observed using flow cytometry. G1-acting cell cycle genes (P21, P27 and P53) are overexpressed (p<0.05 to p<0.001), while CDK2 is downregulated upon Indole-3-carbinol treatment (p<0.01 to p<0.001)[1].Indole-3-carbinol suppresses NF-κB activity and stimulates the p53 pathway in pre-B acute lymphoblastic leukemia cells[2].
Cell Assay	THP-1 cells are cultured in RPMI 1640 supplemented with 10% FBS, 100 U/mL penicillin, 100 mg/mL streptomycin and 2 mM Glutamax in a fully humidified atmosphere with 5% CO2. Cells (2-5×105 mL-1) are seeded in six well plates followed by resuspension in complete growth media. THP-1 monocyte cells are then treated with 10 ng/mL phorbol 12-myristate 13-acetate as a tumor promoter to induce stable differentiation into attaching macrophage-like cells and overexpression of AhR. The cells are then treated with varying concentrations of Indole-3-carbinol (1, 10 and 100 μM, and 1 mM). THP-1 cells and enriching normal monocytes are seeded at 5×104 cells/well in 24-well plate with different concentrations of Indole-3-carbinol and observed for 24 and 48 h followed by MTT assay. The cells are incubated in triplicates in a final volume of 200 mL of Phenol Red free RPMI 1640 for 20 h . An aliquot of 20 mL of MTT solution (5 mg/mL) is added to each well and incubated for 4 h. Formazan crystals are formed. An amount of 300 mL DMSO is then added to each well as a cell lysis solution. Percentage of cell viability is assessed by spectrophotometry at 570 nm[1].
References	[1]. Mohammadi S, et al. Indole-3-carbinol induces G1 cell cycle arrest and apoptosis through aryl hydrocarbon receptor in THP-1 monocytic cell line.J Recept Signal Transduct Res. 2017 Oct;37(5):506-514. [2]. Safa M, et al. Indole-3-carbinol suppresses NF-κB activity and stimulates the p53 pathway in pre-B acute lymphoblastic leukemia cells. Tumour Biol. 2015 May;36(5):3919-30.

Density	1.3±0.1 g/cm3
Boiling Point	360.6±17.0 °C at 760 mmHg
Melting Point	96-99 °C(lit.)
Molecular Formula	C₉H₉NO
Molecular Weight	147.174
Flash Point	171.9±20.9 °C
Exact Mass	147.068420
PSA	36.02000
LogP	0.96
Vapour Pressure	0.0±0.8 mmHg at 25°C
Index of Refraction	1.705
Storage condition	2-8°C
Stability	2-80C

Indole-3-carbinol MSDS(Chinese)

CHEMICAL IDENTIFICATION

RTECS NUMBER :: NL9483000

CHEMICAL NAME :: Indole-3-methanol

CAS REGISTRY NUMBER :: 700-06-1

BEILSTEIN REFERENCE NO. :: 0121323

LAST UPDATED :: 199706

DATA ITEMS CITED :: 5

MOLECULAR FORMULA :: C9-H9-N-O

MOLECULAR WEIGHT :: 147.19

WISWESSER LINE NOTATION :: T56 BMJ D1Q

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Subcutaneous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 500 mg/kg

TOXIC EFFECTS :: Behavioral - coma

REFERENCE :: FCTXAV Food and Cosmetics Toxicology. (London, UK) V.1-19, 1963-81. For publisher information, see FCTOD7. Volume(issue)/page/year: 18,159,1980 ** OTHER MULTIPLE DOSE TOXICITY DATA **

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 9100 mg/kg/13W-I

TOXIC EFFECTS :: Liver - jaundice (or hyperbilirubinemia) hepatocellular Liver - changes in liver weight

REFERENCE :: TOXID9 Toxicologist. (Soc. of Toxicology, Inc., 475 Wolf Ledge Parkway, Akron, OH 44311) V.1- 1981- Volume(issue)/page/year: 15,292,1995

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Mammal - dog

DOSE/DURATION :: 5600 mg/kg/13W-I

TOXIC EFFECTS :: Nutritional and Gross Metabolic - weight loss or decreased weight gain

REFERENCE :: TOXID9 Toxicologist. (Soc. of Toxicology, Inc., 475 Wolf Ledge Parkway, Akron, OH 44311) V.1- 1981- Volume(issue)/page/year: 15,292,1995 ** REPRODUCTIVE DATA **

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 1 mg/kg

SEX/DURATION :: female 15 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Newborn - delayed effects

REFERENCE :: TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 141,68,1996

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Subcutaneous

DOSE :: 400 mg/kg

SEX/DURATION :: female 8-9 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus)

REFERENCE :: FCTXAV Food and Cosmetics Toxicology. (London, UK) V.1-19, 1963-81. For publisher information, see FCTOD7. Volume(issue)/page/year: 18,159,1980

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H315-H319
Precautionary Statements	P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xi:Irritant
Risk Phrases	R36/38
Safety Phrases	S26-S36
RIDADR	NONH for all modes of transport
WGK Germany	3
RTECS	NL9483000
HS Code	2933990090

Precursor 10
CAS#:487-89-8 Indole-3-carbox... CAS#:124-40-3 Dimethylamine CAS#:120-72-9 Indole CAS#:50-00-0 Formaldehyde
CAS#:86204-54-8 indolyl-3-methy... CAS#:17206-03-0 1H-Indole-3-met... CAS#:87-52-5 Gramine CAS#:86204-60-6 indol-3ylmethan...
CAS#:87-51-4 3-Indoleacetic acid CAS#:5457-31-8 1H-indol-3-ylme...
DownStream 9
CAS#:487-89-8 Indole-3-carbox... CAS#:5457-28-3 3-Cyanoindole CAS#:91-56-5 isatin CAS#:22546-09-4 3-(1H-indol-3-y...
CAS#:83-34-1 3-Methylindole CAS#:4414-76-0 1H-Indole-3-pro... CAS#:96551-22-3 1-Boc-3-Hydroxy... CAS#:771-50-6 1H-Indole-3-car...
CAS#:771-51-7 3-Indoleacetonitrile

HS Code	2933990090
Summary	2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

The extended version of restriction analysis approach for the examination of the ability of low-molecular-weight compounds to modify DNA in a cell-free system.

Food Chem. Toxicol. 75 , 118-27, (2015)

One of the primary requirements in toxicology is the assessment of ability of chemicals to induce DNA covalent modification. There are several well-established methods used for this purpose such as (3...

Indole-3-carbinol inhibits tumorigenicity of hepatocellular carcinoma cells via suppression of microRNA-21 and upregulation of phosphatase and tensin homolog.

Biochim. Biophys. Acta 1853(1) , 244-53, (2015)

A major obstacle to successful treatment of hepatocellular carcinoma (HCC) is its high resistance to cytotoxic chemotherapy due to overexpression of multidrug resistance genes. Activation of the AKT p...

In vitro activity of glucosinolates and their degradation products against brassica-pathogenic bacteria and fungi.

Appl. Environ. Microbiol. 81(1) , 432-40, (2014)

Glucosinolates (GSLs) are secondary metabolites found in Brassica vegetables that confer on them resistance against pests and diseases. Both GSLs and glucosinolate hydrolysis products (GHPs) have show...

Indole-3-carbidol

MFCD02683932

3-INDOLAMETHANOL

3-indolemethanol

1H-Indole-3-methanol (9CI)

Indole-3-carbinol

EINECS 211-836-2

3-IndoleCarbinol

1H-Indol-3-ylmethanol

1H-Indole-3-methanol

(1H-indol-3-yl)-methanol

(1H-indole-3-yl)-methanol

indole-3-methanol

I3C

3-hydroxymethyl-1H-indole

3-INDOLYLMETHANOL

3-indolylcarbinol

The content on this webpage is sourced from various professional data sources. If you have any questions or concerns regarding the content, please feel free to contact service1@chemsrc.com.

Shanghai Nianxing Industrial Co., Ltd
China
Product Name: Indole-3-carbinol
Price: Check
Purity: 98.0%
Delivery: 10 Day
Contact: Yang
Email: sales@echemcloud.com

Shanghai Jizhi Biochemical Technology Co., Ltd
China
Product Name: Indole-3-carbinol
Price: ￥568.0/100g ￥178.0/25g
Purity: 98.0%
Delivery: 2 Day
Contact: Liu jia
Email: 2130147988@qq.com

DC Chemicals Limited
China
Product Name: Indole-3-carbinol
Price: $250.0/100mg $500.0/250mg $1000.0/1g
Purity: 98.0%
Delivery: 3 Day
Contact: Tony Cao
Email: sales@dcchemicals.com

ShangHai DC Chemicals Co.,Ltd
China
Product Name: Indole-3-carbinol
Price: ￥Inquiry/1g
Purity: 98.9%
Delivery: 1 Day
Contact: Tony Lai
Email: order@dcchemicals.com

Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: Indole-3-carbinol
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
Delivery: Inquiry
Contact: Ms Wang
Email: enquiry@dycnchem.com

Get all suppliers and price by the below link:

Indole-3-carbinol suppliers

Price: ￥53.9/5g

Reference only. check more Indole-3-carbinol price

Indole-3-carbinol

Use of Indole-3-carbinol

Names