PSI-6130
Modify Date: 2024-01-04 01:03:42
PSI-6130 structure
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Common Name | PSI-6130 | ||
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| CAS Number | 817204-33-4 | Molecular Weight | 259.234 | |
| Density | 1.7±0.1 g/cm3 | Boiling Point | 482.4±55.0 °C at 760 mmHg | |
| Molecular Formula | C10H14FN3O4 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | 245.5±31.5 °C | |
Use of PSI-6130PSI-6130 is a potent and selective inhibitor of HCV NS5B polymerase, and inhibits HCV replication with a mean IC50 of 0.6 μM. |
| Name | 4-amino-1-[(2R,3R,4R,5R)-3-fluoro-4-hydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl]pyrimidin-2-one |
|---|---|
| Synonym | More Synonyms |
| Description | PSI-6130 is a potent and selective inhibitor of HCV NS5B polymerase, and inhibits HCV replication with a mean IC50 of 0.6 μM. |
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| Related Catalog | |
| Target |
IC50: 0.6 μM (HCV replication)[2] |
| In Vitro | PSI-6130 exhibits potent and specific inhibitory activity against HCV RNA replication mediated by the NS5B polymerase. Both PSI-6130 inhibit HCV GT-1b (Con1 strain) and GT-1a (H77 strain) subgenomic RNA replication, with mean EC50 values of 0.51 and 0.30 μM, respectively. PSI-6130 inhibits 40% human serum with EC50 of 0.51 μM[1]. PSI-6130 inhibits HCV replication with a mean IC50 of 0.6 μM, PSI-6130-TP inhibits HCV replicase with a mean IC50 of 0.34 μM. PSI-6130-TP inhibits recombinant HCV Con1 NS5B on a heteropolymeric RNA template derived from the 3′-end of the negative strand of the HCV genome with an IC50 of 0.13 μM and Ki of 0.023 μM[2]. |
| Kinase Assay | The inhibition potency of compounds with respect to the RdRp activity of recombinant NS5B570-BK, NS5B570-Con1, and NS5B570-H77 proteins is determined by measuring the incorporation of radiolabeled NMP into acid-insoluble RNA products by use of a complement strand of internal ribosomal entry site (cIRES) RNA template. Briefly, 50% inhibitory concentration (IC50) determinations are carried out using 200 nM in vitro-transcribed cIRES RNA template, 1 μCi of tritiated UTP (42 Ci/mmol), 500 μM ATP, 500 μM GTP, 1 μM CTP, 1× TMDN buffer (40 mM Tris-HCl [pH 8.0], 4 mM MgCl2, 4 mM dithiothreitol, 40 mM NaCl), and 200 nM enzyme. The inhibition potency of compounds with respect to the RdRp activity of NS5B570-S282T-Con1 is determined under GT-1b assay conditions as. NS5B570-BK and NS5B570-Con1 enzymes are used as controls. The final reaction volume is 50 μL under all assay conditions. All reactions contain a final 10% dimethyl sulfoxide. Km and Ki values are measured. |
| References |
| Density | 1.7±0.1 g/cm3 |
|---|---|
| Boiling Point | 482.4±55.0 °C at 760 mmHg |
| Molecular Formula | C10H14FN3O4 |
| Molecular Weight | 259.234 |
| Flash Point | 245.5±31.5 °C |
| Exact Mass | 259.096832 |
| PSA | 110.60000 |
| LogP | -0.72 |
| Vapour Pressure | 0.0±2.7 mmHg at 25°C |
| Index of Refraction | 1.653 |
|
~93%
PSI-6130 CAS#:817204-33-4 |
| Literature: Carr, Robert; Hildbrand, Stefan; Hodges, Mark L.; Kammerer, Michael; Lang, John F.; Lawrimore, III, William J.; Nguyen, Dieu Patent: US2013/324709 A1, 2013 ; Location in patent: Paragraph 0053; 0054 ; |
| Precursor 1 | |
|---|---|
| DownStream 1 | |
| 2'-Deoxy-2'-fluoro-2'-methylcytidine |
| Cytidine, 2'-deoxy-2'-fluoro-2'-methyl- |
| 2'-Fluoro-2'-C-methylcytidine |
| 2'-deoxy-2'-fluoro-2'-C-methylcytidine |
| PSI6130 |
| R-1656 |
| PSI-6130 |