Bovine Serum Albumin
Bovine Serum Albumin structure
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Common Name | Bovine Serum Albumin | ||
|---|---|---|---|---|
| CAS Number | 9048-46-8 | Molecular Weight | N/A | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | N/A | Melting Point | N/A | |
| MSDS | USA | Flash Point | N/A | |
Use of Bovine Serum AlbuminBovine Serum Albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. BSA is a globular protein that is used in numerous biochemical applications. |
| Name | Serum albumin |
|---|---|
| Synonym | More Synonyms |
| Description | Bovine Serum Albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. BSA is a globular protein that is used in numerous biochemical applications. |
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| Related Catalog | |
| In Vitro | Bovine serum albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. Bovine serum albumin constitutes ca. 60% of all plasma protein and binds and transports a large number of physiological and non-physiological ligands. Bovine serum albumin contains 17 disulfide bonds and one unpaired cysteine (Cys34), which facilitates dimerization and also influences higher-order association, since the rate of aggregation is slowed down if Cys34 is covalently bound to another compound. MTT assay for fibril cytotoxicity shows Bovine Serum Albumin is beneficial for cell growth irrespective of its aggregated state. Lack of cytotoxicity is confirmed by membrane permeabilization assays. In the subsequent 40 h, an increase in the viability of the treated cells of 300-400% is observed, indicating that the cells incubated with Bovine Serum Albumin achieve a higher viability than the untreated cells[1]. |
| Cell Assay | Samples are prepared in 96-well microplates and all wells without samples are filled with 200 μL PBS to minimize evaporation. To each well is loaded 100 μL SH-SY5Y growth medium with or without 10.000 SH-SY5Y neuroblastoma cells. The assay is started by adding 25 μL buffer, with or without 5 mg/mL sonicated Bovine Serum Albumin previously incubated for 0, 2 or 96 h at 70°C (giving a final BSA concentration of 1 mg/mL). All sample combinations are loaded in triplets, and for each of the six Bovine Serum Albumin combinations a corresponding triplet is loaded without cells, to be used as blank. One triplet is also loaded without both cells and BSA and one only without Bovine Serum Albumin[1]. |
| References |
| Storage condition | 2~8°C |
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| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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| Hazard Codes | Xn |
| Risk Phrases | 22-52/53-21/22 |
| Safety Phrases | 24/25-61-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| RTECS | MT6446000 |
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Fibronectin stimulates human sperm capacitation through the cyclic AMP/protein kinase A pathway.
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The dynactin complex enhances the speed of microtubule-dependent motions of adenovirus both towards and away from the nucleus.
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| Albumin |
| MFCD00804577 |
| Bovine Serum Albumin |
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