硫氰酸胍结构式
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常用名 | 硫氰酸胍 | 英文名 | Guanidine thiocyanate |
|---|---|---|---|---|
| CAS号 | 593-84-0 | 分子量 | 118.16 | |
| 密度 | 1.103 g/mL at 20 °C | 沸点 | 132.9ºC at 760 mmHg | |
| 分子式 | C2H6N4S | 熔点 | 120-122 °C(lit.) | |
| MSDS | 中文版 美版 | 闪点 | 34.2ºC | |
| 符号 |
GHS05, GHS07 |
信号词 | Danger |
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Efficient programmable gene silencing by Cascade.
Nucleic Acids Res. 43(1) , 237-46, (2015) Methods that permit controlled changes in the expression of genes are important tools for biological and medical research, and for biotechnological applications. Conventional methods are directed at individually changing each gene, its regulatory elements or ... |
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Isolation and immortalization of MIP-GFP neurons from the hypothalamus.
Endocrinology 155(6) , 2314-9, (2014) The mouse insulin I promoter (MIP) construct was developed to eliminate the promoter activity detected with the rat insulin II promoter in specific hypothalamic neurons that may have unintended effects on glucose and energy homeostasis in transgenic models. T... |
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Deficiency of the novel exopolyphosphatase Rv1026/PPX2 leads to metabolic downshift and altered cell wall permeability in Mycobacterium tuberculosis.
MBio 6 , e02428, (2015) Mycobacterium tuberculosis can persist for decades in the human host. Stringent response pathways involving inorganic polyphosphate [poly(P)], which is synthesized and hydrolyzed by polyphosphate kinase (PPK) and exopolyphosphatase (PPX), respectively, are be... |
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The floral transcriptome of Eucalyptus grandis.
New Phytol. 206 , 1406-22, (2015) As a step toward functional annotation of genes required for floral initiation and development within the Eucalyptus genome, we used short read sequencing to analyze transcriptomes of floral buds from early and late developmental stages, and compared these wi... |
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MTCH2 is differentially expressed in rat testis and mainly related to apoptosis of spermatocytes.
Cell Tissue Res. 361 , 869-83, (2015) MTCH2 has been described in liver as a protein involved in the intrinsic apoptotic pathway, although new evidence also associates this protein with cellular metabolism. In this work, the expression of MTCH2 in testis (an organ in which high levels of apoptosi... |
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Evaluation of two methods for quantification of hsp70 mRNA from the waterborne pathogen Cryptosporidium parvum by reverse transcription real-time PCR in environmental samples.
Water Res. 43(10) , 2669-78, (2009) We optimized and evaluated two mRNA extraction methods to quantify induced hsp70 mRNA from viable and injured Cryptosporidium parvum oocysts by reverse transcription quantitative real-time PCR (RT-qPCR) in raw and treated manure. Methods based on guanidinium ... |
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A DNA extraction procedure that allows mite specimens to be slide mounted: phytoseiid species evaluated as a model.
Exp. Appl. Acarol. 52(2) , 131-40, (2010) Four protocols for extracting DNA from mites, using phytoseiid species as exemplars, were evaluated to determine whether the DNA obtained could be used to amplify nuclear, mitochondrial or Random Amplified Polymorphic DNA (RAPD) markers from males, females an... |
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Using silica particles to isolate total RNA from plant tissues recalcitrant to extraction in guanidine thiocyanate.
Anal. Biochem. 374(2) , 426-8, (2008) The most commonly used protocol of the RNA isolation, the guanidine thiocyanate method, was unsuitable for recalcitrant plant tissues containing a large amount of storage proteins and secondary metabolites. We demonstrated that RNA could bind to the silica pa... |
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Distinct effects of guanidine thiocyanate on the structure of superfolder GFP.
PLoS ONE 7(11) , e48809, (2012) Having a high folding efficiency and a low tendency to aggregate, the superfolder GFP (sfGFP) offers a unique opportunity to study the folding of proteins that have a β-barrel topology. Here, we studied the unfolding-refolding of sfGFP that was induced by gua... |
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The proline rich homeodomain protein PRH/Hhex forms stable oligomers that are highly resistant to denaturation.
PLoS ONE 7(4) , e35984, (2012) Many transcription factors control gene expression by binding to specific DNA sequences at or near the genes that they regulate. However, some transcription factors play more global roles in the control of gene expression by altering the architecture of secti... |