6-氨基青霉烷酸

Prep Biochem Biotechnol. 40(1) , 38-45, (2010)

Enzymatic hydrolysis of penicillin G for production of 6-amino-penicillanic-acid (6-APA) was achieved by using penicillin G acylase as catalyst in an aqueous-methylisobutyl ketone (MIBK) system. The optimization was carried out and it was found that the best ...

Bioprocess Biosyst. Eng. 33(5) , 557-64, (2010)

The complexity of biological processes often makes impractical the development of detailed, structured phenomenological models of the cultivation of microorganisms in bioreactors. In this context, data pre-treatment techniques are useful for bioprocess contro...

Bioorg. Med. Chem. Lett. 19(14) , 3787-90, (2009)

We report the first synthesis of a 5S penem, known to bind bacterial type I signal peptidase, from the commercially available and inexpensive 6-aminopenicillanic acid. We report the first in vivo activity of the compound and use structure-activity relationshi...

Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 75(1) , 183-90, (2010)

Quantum mechanical calculations of energies, geometries and vibrational wavenumbers of 6-aminopenicillanic acid were carried out by using ab initio HF and density functional theory (DFT/B3LYP) methods with 6-311G(d,p) basis set. The optimized geometrical para...

Appl. Microbiol. Biotechnol. 88(1) , 49-55, (2010)

A one-pot, two-step enzymatic synthesis of amoxicillin from penicillin G, using penicillin acylase, is presented. Immobilized penicillin acylase from Kluyvera citrophila was selected as the biocatalyst for its good pH stability and selectivity. Hydrolysis of ...

Arch. Biochem. Biophys. 481(1) , 45-51, (2009)

The functionality of regions within the equatorial domain of Group II chaperonins is poorly understood. Previously we showed that a 70 amino acid sequence within this domain on the single-subunit recombinant thermosome from Methanocaldococcus jannaschii (rTHS...

Bioprocess Biosyst. Eng. 34(9) , 1103-17, (2011)

The article deals with experimental determination of ionization constants and solubility for the compounds (target products, initial β-lactams, acylating agents and by-products) involved in enzymatic synthesis of some therapeutically used aminopenicillins and...

Appl. Biochem. Biotechnol. 144(2) , 145-59, (2008)

A dodecane/thermosensitive polymer/water three-liquid-phase system was introduced for enzymatic hydrolysis of penicillin G (Pen G) for 6-aminopenicillanic acid (6-APA). The enzyme was covalently attached to the terminal of PEO-PPO-PEO polymer (L63), which wou...

Appl. Biochem. Biotechnol. 160(7) , 2045-53, (2010)

The production of extracellular and mycelia-associated penicillin G acylase (maPGA) with Mucor griseocyanus H/55.1.1 by surface-adhesion fermentation using Opuntia imbricata, a cactus, as a natural immobilization support was studied. Enzyme activity to form 6...

Indian J. Exp. Biol. 49(4) , 289-92, (2011)

In the present study different bacterial samples were isolated from soil of different places of Dibrugarh and screened for biotransformation ability to produce 6-Aminopenicillanic acid. Among ten isolated bacterial samples, three gram positive bacterial sampl...