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耐尔兰

耐尔兰用途

Nile Blue A可用于区分黑色素和脂褐素。它也可用于脂肪染色,以及制备电流葡萄糖传感器。

耐尔兰名称

[ CAS 号 ]:
3625-57-8

[ 中文名 ]:
硫酸耐尔蓝

[ 英文名 ]:
Nile Blue A (sulfate)

[中文别名 ]:

[英文别名 ]:

EINECS 222-832-5
Nile Blue A sulfate
MFCD00064529
Nile Blue A
Basic Blue 12,Nile blue sulfate
Nile Blue A,certified
Nile blue, sulfate
Basic Blue-12
Basic Blue 12 Nile blue sulfate
Nile blue sulfate

耐尔兰生物活性

[ 描述 ]:

Nile Blue A可用于区分黑色素和脂褐素。它也可用于脂肪染色,以及制备电流葡萄糖传感器。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

尼罗蓝A是碱性恶嗪染料,可溶于水和乙醇。尼罗蓝A是细菌中PHB颗粒的令人满意的染色剂,实际上为此目的优于苏丹黑B.当用尼罗蓝A染色时,聚-β-羟基丁酸酯颗粒显示出强烈的橙色荧光。尼罗蓝A似乎比苏丹黑B染色更多的PHB颗粒,并且通过脱色程序不容易从细胞中洗脱[1]。尼罗蓝A用作聚羟基链烷酸积累微生物的染色剂或用于检测微生物中的聚羟基链烷酸。不积累可检测的多羟基链烷酸的大肠杆菌细胞可以用尼罗蓝A染色,并且该染色足以在荧光激活细胞分选(FACS)实验中鉴定这些细胞。尼罗蓝A染色不影响肽的表面展示或通过第二荧光特异性标记这些肽。使用尼罗蓝A染色大肠杆菌用于流式细胞术是一种易于处理和低成本的替代其他荧光染料或细胞内表达的例如绿色荧光蛋白[2]。尼罗蓝A是研究最多的苯并吩嗪染料之一,作为光动力疗法的有效光敏剂。当静脉内给药时,染料通过血液循环分散到全身,被大多数细胞吸收,强调它与各种生物分子的相互作用[3]。

[细胞实验]

制备1％尼罗蓝A水溶液并在使用前过滤。可能需要温和加热以完全溶解污渍。将热固定的细菌细胞涂片用尼罗蓝A溶液在55℃下在coplin染色罐中染色10分钟。染色后，用自来水洗涤载玻片以除去过量的污渍并用8％乙酸水溶液洗涤1分钟。将染色的涂片洗净并用吸水纸吸干，再用自来水重新涂抹，并用一个涂层覆盖。 1个玻璃盖板。用具有反射荧光附着物的Nikon Labphot显微镜检查制剂[1]。用尼罗蓝A染色PHA-菌株大肠杆菌UT5600（DE3）。在Erlenmeyer烧瓶中，用一个UT5600（DE3）菌落接种20mL Luria-Bertani（LB）肉汤，并在37°温育14小时。 C和200转/分钟。随后，用200μL14-h培养物接种20mL含有尼罗蓝A的终浓度为0.5μg/ mL的LB肉汤，并培养至578nm（OD578）为0.6的光密度。作为对照，用200μL14小时培养物接种20mL LB肉汤（不含尼罗蓝A），并且还培养至OD578为0.6的光密度。每20分钟，确定两种培养物的OD578，以验证染料是否对细菌的生长有任何影响[2]。使用乙醇作为溶剂制备尼罗蓝A储备溶液。对于所有研究，NB的浓度维持在5μM。将溶液放置1小时以在光谱测量之前达到平衡。使用Shimadzu分光光度计（UV-1800）记录吸收光谱，并使用Jobin-Yvon Spectrofluorimeter记录发射光谱。使用450nm纳米LED作为光源，并且在λem= 672nm处收集荧光寿命[3]。

[参考文献]

[1]. Ostle AG, et al. Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Appl Environ Microbiol. 1982 Jul;44(1):238-41.

[2]. Betscheider D, et al. Nile blue A for staining Escherichia coli in flow cytometer experiments. Anal Biochem. 2009 Jan 1;384(1):194-6.

[3]. Mishra SS, et al. Spectroscopic investigation of interaction of Nile Blue A, a potent photosensitizer, with bile salts in aqueous medium. J Photochem Photobiol B. 2014 Dec;141:67-75.

[相关活性小分子]

耐尔兰物理化学性质

[ 沸点 ]:
487.9ºC

[ 熔点 ]:
>300ºC (dec.)(lit.)

[ 分子式 ]:
C₂₀H₂₀N₃O₃S_0.5

[ 分子量 ]:
366.42

[ 闪点 ]:
248.8ºC

[ PSA ]:
198.76000

[ LogP ]:
7.86520

[ 外观性状 ]:
暗绿色至黑色粉末

[ 储存条件 ]:
室温

[ 水溶解性 ]:
Soluble in water, ethanol

耐尔兰MSDS

详细MSDS(安全技术说明书)

耐尔兰毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: DJ1931000

CAS REGISTRY NUMBER :: 3625-57-8

LAST UPDATED :: 199512

DATA ITEMS CITED :: 3

MOLECULAR FORMULA :: C40-H40-N6-O2.O4-S

MOLECULAR WEIGHT :: 732.92

WISWESSER LINE NOTATION :: T D6 B666 CN JOJ GN2&2 MZ 2 &.S-O4 &15/33

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD - Lethal dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: >500 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: CBCCT* "Summary Tables of Biological Tests," National Research Council Chemical-Biological Coordination Center. (National Academy of Science Library, 2101 Constitution Ave., NW, Washington, DC 20418) Volume(issue)/page/year: 4,46,1952

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 27 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 44,225,1978 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X4351 No. of Facilities: 6 (estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees: 39 (estimated) No. of Female Employees: 20 (estimated)

耐尔兰安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xi: Irritant;

[ 安全声明 (欧洲) ]:
S24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ RTECS号 ]:
DJ1931000

[ 海关编码 ]:
2934999090

耐尔兰海关

[ 海关编码 ]: 2934999090

[ 中文概述 ]:
2934999090. 其他杂环化合物. 增值税率:17.0%. 退税率:13.0%. 监管条件:无. 最惠国关税:6.5%. 普通关税:20.0%

[ 申报要素 ]: 品名, 成分含量, 用途

[ Summary ]:
2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

耐尔兰文献

Heterogeneous immunoassay for soy protein determination using nile blue-doped silica nanoparticles as labels and front-surface long-wavelength fluorimetry.

Anal. Chim. Acta 701(2) , 194-9, (2011)

A long-wavelength fluoroimmunoassay for the determination of soy protein is reported for the first time using a conjugate composed of anti-soy protein antibodies bound to nile blue-doped silica nanopa...

Stepwise fluorescence changes of quantum dots: single-molecule spectroscopic studies on the properties of turn-on quantum dots.

Chem. Commun. (Camb.) 48(5) , 723-5, (2012)

Single-molecule spectroscopy of turn-on quantum dots induced by NADPH-dependent biocatalyzed transformations reveals that the fluorescence intensities of quantum dots functionalized with Nile Blue are...

Rapid separations of nile blue stained microorganisms as cationic charged species by chip-CE with LIF.

Electrophoresis 33(9-10) , 1421-6, (2012)

Rapid detection of microorganisms by alternative methods is desirable. Electromigration separation methods have the capability to separate microorganisms according to their charge and size and laser-i...

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耐尔兰