5-(Bromomethyl)fluorescein

5-(Bromomethyl)fluorescein Structure
5-(Bromomethyl)fluorescein structure
Common Name 5-(Bromomethyl)fluorescein
CAS Number 148942-72-7 Molecular Weight 425.22900
Density 1.79g/cm3 Boiling Point 681.8ºC at 760mmHg
Molecular Formula C21H13BrO5 Melting Point N/A
MSDS Chinese USA Flash Point 366.2ºC

Labeling the (Ca(2+)-Mg2+)-ATPase of sarcoplasmic reticulum at Glu-439 with 5-(bromomethyl)fluorescein.

Biochemistry 32 , 6095, (1993)

The (Ca(2+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum was labeled with 5-(bromomethyl)fluorescein. A stoichiometry of one label per ATPase molecule was found, which was unaffected by the presence of ATP. Labeling resulted in a 60% decrease in ATP...

Effect of magnetic nanoparticles on tobacco BY-2 cell suspension culture.

Int. J. Environ. Res. Public Health 10 , 47-71, (2013)

Nanomaterials are structures whose exceptionality is based on their large surface, which is closely connected with reactivity and modification possibilities. Due to these properties nanomaterials are used in textile industry (antibacterial textiles with silve...

Rapid long-range proton diffusion along the surface of the purple membrane and delayed proton transfer into the bulk.

Proc. Natl. Acad. Sci. U. S. A. 92(2) , 372-6, (1995)

The pH-indicator dye fluorescein was covalently bound to the surface of the purple membrane at position 72 on the extracellular side of bacteriorhopsin and at positions 101, 105, 160, or 231 on the cytoplasmic side by reacting bromomethylfluorescein with the ...

5-Bromomethyl fluorescein (5-BMF) for derivatization of carboxyl containing analytes for use with laser-induced fluorescence detection.

Pharm. Res. 12(6) , 930-6, (1995)

5-Bromomethyl fluorescein (5-BMF) was evaluated in this work as a pre-column, off-line derivatizing reagent for analytes containing a free carboxyl group. The reagent possessed high molar absorptivity and quantum yield and it's excitation maximum matched the ...

Measurement of thiol-containing amino acids and phytochelatin (PC2) via capillary electrophoresis with laser-induced fluorescence detection.

Electrophoresis 23(1) , 81-7, (2002)

An analytical method for determining thiols and phytochelatins using high-performance capillary electrophoresis coupled with laser-induced fluorescence detection is presented. The technique utilizes the labeling of thiols with the fluorescent reagent 5-bromom...

Analysis of gamma-(cholesteryloxy)butyric acid in biologic samples by derivatization with 5-(bromomethyl)fluorescein followed by high-performance liquid chromatography with laser-induced fluorescence detection.

Anal. Chem. 68(2) , 327-32, (1996)

This report describes the first application of 5-(bromo-methyl)fluorescein (5-BMF) for the quantitation of a pharmaceutically relevant carboxyl-containing analyte in a biological matrix. An analytical method for quantitation of gamma-(cholesteryloxy)butyric a...

Localization of the hinge region of the Ca(2+)-ATPase of sarcoplasmic reticulum using resonance energy transfer.

Biochim. Biophys. Acta 1192(1) , 53-60, (1994)

The Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum can be labelled at Cys-670 and Cys-674 with 5-[[2-[(iodoacetyl) amino]ethyl]amino]naphthalene-1-sulphonic acid (IAEDANS). Resonance energy transfer has been used to measure the distance between Cys-6...

Iron-sulfur cluster biosynthesis: characterization of IscU-IscS complex formation and a structural model for sulfide delivery to the [2Fe-2S] assembly site.

J. Biol. Inorg. Chem. 14(6) , 829-39, (2009)

Recent work on the bacterial iron-sulfur cluster (isc) family of gene products, and eukaryotic homologs, has advanced the molecular understanding of cellular mechanisms of iron-sulfur cluster biosynthesis. Members of the IscS family are pyridoxyl-5'-phosophat...

T. Holletz et al.

Liebigs Ann. Chem. , 1051, (1993)

P.S. Mukherjee, H.T. Karnes

Anal. Chem. 68 , 327, (1993)