5-bromo-4-chloro-3-indolyl β-D-galactoside

5-bromo-4-chloro-3-indolyl β-D-galactoside Structure
5-bromo-4-chloro-3-indolyl β-D-galactoside structure
Common Name 5-bromo-4-chloro-3-indolyl β-D-galactoside
CAS Number 7240-90-6 Molecular Weight 408.629
Density 1.9±0.0 g/cm3 Boiling Point 673.9±0.0 °C at 760 mmHg
Molecular Formula C14H15BrClNO6 Melting Point 230 °C
MSDS USA Flash Point 361.3±0.0 °C

Pericytes in the myovascular niche promote post-natal myofiber growth and satellite cell quiescence.

Development 142(7) , 1242-53, (2015)

The satellite cells, which serve as adult muscle stem cells, are both located beneath myofiber basement membranes and closely associated with capillary endothelial cells. We observed that 90% of capillaries were associated with pericytes in adult mouse and hu...

Engineering the genome of Thermus thermophilus using a counterselectable marker.

J. Bacteriol. 197(6) , 1135-44, (2015)

Thermus thermophilus is an extremely thermophilic bacterium that is widely used as a model thermophile, in large part due to its amenability to genetic manipulation. Here we describe a system for the introduction of genomic point mutations or deletions using ...

A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning.

Nat. Protoc. 4 , 341-55, (2009)

The extreme strength and elasticity of spider silks originate from the modular nature of their repetitive proteins. To exploit such materials and mimic spider silks, comprehensive strategies to produce and spin recombinant fibrous proteins are necessary. This...

Microbiological analysis of pre-packed sweet basil (Ocimum basilicum) and coriander (Coriandrum sativum) leaves for the presence of Salmonella spp. and Shiga toxin-producing E. coli.

Int. J. Food Microbiol. 208 , 11-8, (2015)

Enteric pathogens, such as Salmonella spp. and pathogenic Escherichia coli, have been detected and associated with food borne outbreaks from (imported) fresh leafy herbs. Screening on imported herbs from South East Asian countries has been described. However,...

Functional microRNA library screening identifies the hypoxamir miR-24 as a potent regulator of smooth muscle cell proliferation and vascularization.

Antioxid. Redox Signal. 21(8) , 1167-76, (2014)

Smooth muscle cells (SMCs) are key components within the vasculature. Dependent on the stimulus, SMC can either be in a proliferative (synthetic) or differentiated state. Alterations of SMC phenotype also appear in several disease settings, further contributi...

Possible introgression of B chromosomes between bee species (Genus Partamona ).

Cytogenet. Genome Res. 144(3) , 220-6, (2015)

The origin of supernumerary (B) chromosomes is still a debated topic, with intra- and interspecific origins being the most plausible options. In the bee Partamona helleri, a sequence-characterized amplified region (SCAR) marker being specific to B chromosomes...

Method for screening compounds that influence virulence gene expression in Staphylococcus aureus.

Antimicrob. Agents Chemother. 54 , 509-12, (2010)

We present a simple assay to examine effects of compounds on virulence gene expression in the human pathogen Staphylococcus aureus. The assay employs transcriptional reporter strains carrying lacZ fused to central virulence genes. Compounds affecting virulenc...

X-gal staining of the central nervous system in adult Drosophila.

Cold Spring Harb. Protoc. 2012(2) , 239-41, (2012)

The Drosophila nervous system provides a valuable model for studying various aspects of brain development and function. The postembryonic Drosophila brain is especially useful, because specific neuron types derive from specific progenitors at specific times. ...

NrdI essentiality for class Ib ribonucleotide reduction in Streptococcus pyogenes.

J. Bacteriol. 190 , 4849-58, (2008)

The Streptococcus pyogenes genome harbors two clusters of class Ib ribonucleotide reductase genes, nrdHEF and nrdF*I*E*, and a second stand-alone nrdI gene, designated nrdI2. We show that both clusters are expressed simultaneously as two independent operons. ...

The vascular endothelium of the adipose tissue gives rise to both white and brown fat cells.

Cell Metab. 15(2) , 222-9, (2012)

Adipose tissue expansion involves the enlargement of existing adipocytes, the formation of new cells from committed preadipocytes, and the coordinated development of the tissue vascular network. Here we find that murine endothelial cells (ECs) of classic whit...