PLoS ONE 2015-01-01

Tumor Repression of VCaP Xenografts by a Pyrrole-Imidazole Polyamide.

Amanda E Hargrove, Thomas F Martinez, Alissa A Hare, Alexis A Kurmis, John W Phillips, Sudha Sud, Kenneth J Pienta, Peter B Dervan

Index: PLoS ONE 10 , e0143161, (2015)

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Abstract

Pyrrole-imidazole (Py-Im) polyamides are high affinity DNA-binding small molecules that can inhibit protein-DNA interactions. In VCaP cells, a human prostate cancer cell line overexpressing both AR and the TMPRSS2-ERG gene fusion, an androgen response element (ARE)-targeted Py-Im polyamide significantly downregulates AR driven gene expression. Polyamide exposure to VCaP cells reduced proliferation without causing DNA damage. Py-Im polyamide treatment also reduced tumor growth in a VCaP mouse xenograft model. In addition to the effects on AR regulated transcription, RNA-seq analysis revealed inhibition of topoisomerase-DNA binding as a potential mechanism that contributes to the antitumor effects of polyamides in cell culture and in xenografts. These studies support the therapeutic potential of Py-Im polyamides to target multiple aspects of transcriptional regulation in prostate cancers without genotoxic stress.