114458-99-0

114458-99-0 structure

Name: Tetramethylrhodamine-5-iodoacetamide
Chemical Name: 2-[3,6-Bis(dimethylamino)-9-xantheniumyl]-5-[(iodoacetyl)amino]benzoate
CAS Number: 114458-99-0
Molecular Formula: C₂₆H₂₄IN₃O₄
Molecular Weight: 569.391
Catalog: Research Areas Others
Create Date: 2018-03-08 03:32:01
Modify Date: 2024-04-05 09:21:52
Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments[1].

Name	2-[3,6-Bis(dimethylamino)-9-xantheniumyl]-5-[(iodoacetyl)amino]benzoate
Synonyms	Xanthylium, 9-[2-carboxy-4-[(2-iodoacetyl)amino]phenyl]-3,6-bis(dimethylamino)-, inner salt 2-[3,6-Bis(dimethylamino)-9-xantheniumyl]-5-[(iodoacetyl)amino]benzoate

Description	Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios[1].
References	[1]. Tyagarajan K, et al. Thiol-reactive dyes for fluorescence labeling of proteomic samples.Electrophoresis. 2003 Jul;24(14):2348-58.