Tetramethylrhodamine-5-iodoacetamide

Modify Date: 2024-04-05 09:21:52

Tetramethylrhodamine-5-iodoacetamide Structure
Tetramethylrhodamine-5-iodoacetamide structure
Common Name Tetramethylrhodamine-5-iodoacetamide
CAS Number 114458-99-0 Molecular Weight 569.391
Density N/A Boiling Point N/A
Molecular Formula C26H24IN3O4 Melting Point N/A
MSDS N/A Flash Point N/A

 Use of Tetramethylrhodamine-5-iodoacetamide


Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments[1].

 Names

Name 2-[3,6-Bis(dimethylamino)-9-xantheniumyl]-5-[(iodoacetyl)amino]benzoate
Synonym More Synonyms

 Tetramethylrhodamine-5-iodoacetamide Biological Activity

Description Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a thiol-selective reactive dye that is used to non-specifically label proteins via the cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments[1].
Related Catalog
In Vitro Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios[1].
References

[1]. Tyagarajan K, et al. Thiol-reactive dyes for fluorescence labeling of proteomic samples.Electrophoresis. 2003 Jul;24(14):2348-58.

 Chemical & Physical Properties

Molecular Formula C26H24IN3O4
Molecular Weight 569.391
Exact Mass 569.081116
Storage condition 2-8℃

 Synonyms

Xanthylium, 9-[2-carboxy-4-[(2-iodoacetyl)amino]phenyl]-3,6-bis(dimethylamino)-, inner salt
2-[3,6-Bis(dimethylamino)-9-xantheniumyl]-5-[(iodoacetyl)amino]benzoate