1312782-34-5

1312782-34-5 structure

Name	AA26-9
Synonyms	Pyrrolidin-1-yl(1H-1,2,3-triazol-1-yl)methanone MFCD28386498 Methanone, 1-pyrrolidinyl-1H-1,2,3-triazol-1-yl- 1-Pyrrolidinyl(1H-1,2,3-triazol-1-yl)methanone

Description	AA26-9 is a potent and broad spectrum serine hydrolase inhibitor.
Related Catalog	Signaling Pathways >> Metabolic Enzyme/Protease >> Phospholipase Research Areas >> Metabolic Disease
In Vitro	AA26-9 is synthesized based on a piperazine scaffold shown previously to inhibit serine hydrolases in the context of p-nitrophenoxy carbamate. AA26-9-inhibited enzymes originated from diverse functional subclasses of serine hydrolases, including lipases/phospholipases (AADACL1, ABHD6, ESD, FAAH, PAFAH2, LYPLA3), peptidases (APEH, PRCP, CTSA), thioesterases (LYPLA1, LYPLA2), and uncharacterized enzymes (ABHD11, ABHD13, BAT5). AA26-9 inhibits one of its enzyme targets LYPLA1 by covalent carbamoylation of the enzyme’s serine nucleophile (S114). AA26-9 inhibits 1/3 of the over 40 serine hydrolase found in T-cells[1].
Cell Assay	Cells are cultured with 20 μM inhibitor AA26-9 or DMSO as a control for 4 h, lysed, separated into soluble and analyzed by competitive gel-based ABPP[1].
References	[1]. Adibekian A, et al. Click-generated triazole ureas as ultrapotent in vivo-active serine hydrolase inhibitors. Nat Chem Biol. 2011 May 15;7(7):469-78.