4-methylumbelliferyl β-D-glucoside

Modify Date: 2025-08-22 01:49:22

4-methylumbelliferyl β-D-glucoside Structure
4-methylumbelliferyl β-D-glucoside structure
 Common Name 4-methylumbelliferyl β-D-glucoside
CAS Number 18997-57-4 Molecular Weight 338.309
Density 1.5±0.1 g/cm3 Boiling Point 626.9±55.0 °C at 760 mmHg
Molecular Formula C16H18O8 Melting Point 258-263ºC (dec.)
MSDS USA Flash Point 233.9±25.0 °C

 Use of 4-methylumbelliferyl β-D-glucoside


4-Methylumbelliferyl β-D-Glucopyranoside, a β-D-glucoside, is a fluorogenic substrate for β-glucosidase, utilizes to assay β-glucosidase activity[1]. 4-Methylumbelliferyl β-D-Glucopyranoside releases the highly fluorescent 4-methylumbelliferyl (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively[2].

 Names

Name 4-Methylumbelliferyl-β-D-glucopyranoside
Synonym More Synonyms

 4-methylumbelliferyl β-D-glucoside Biological Activity

Description 4-Methylumbelliferyl β-D-Glucopyranoside, a β-D-glucoside, is a fluorogenic substrate for β-glucosidase, utilizes to assay β-glucosidase activity[1]. 4-Methylumbelliferyl β-D-Glucopyranoside releases the highly fluorescent 4-methylumbelliferyl (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively[2].
Related Catalog
Target

4-Methylumbelliferyl β-D-Glucopyranoside is a fluorogenic substrate for β-glucosidase[1].

References

[1]. Smitka CM,et al. Rapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp. J Clin Microbiol. 1989;27(1):203-206.

[2]. Oftedal L, et al. Validation and assessment of preanalytical factors of a fluorometric in vitro assay for glucocerebrosidase activity in human cerebrospinal fluid. Sci Rep. 2020;10(1):22098. Published 2020 Dec 16.

 Chemical & Physical Properties

Density 1.5±0.1 g/cm3
Boiling Point 626.9±55.0 °C at 760 mmHg
Melting Point 258-263ºC (dec.)
Molecular Formula C16H18O8
Molecular Weight 338.309
Flash Point 233.9±25.0 °C
Exact Mass 338.100159
PSA 129.59000
LogP -0.46
Vapour Pressure 0.0±1.9 mmHg at 25°C
Index of Refraction 1.641
InChIKey YUDPTGPSBJVHCN-YMILTQATSA-N
SMILES Cc1cc(=O)oc2cc(OC3OC(CO)C(O)C(O)C3O)ccc12
Storage condition 2-8°C

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes T+
Risk Phrases 26/27/28
Safety Phrases 22-24/25
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 2932999099

 Synthetic Route

 Customs

HS Code 2932999099
Summary 2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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 4-methylumbelliferyl β-D-glucosideBioassay

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Name: Primary cell-based high-throughput screening assay for identification of compounds th...
Source: Johns Hopkins Ion Channel Center
Target: regulator of G-protein signaling 4 isoform 2 [Homo sapiens]
External Id: JHICC_RGS_Act_HTS
Name: Luminescence-based cell-based primary high throughput screening assay to identify ago...
Source: The Scripps Research Institute Molecular Screening Center
Target: mu-type opioid receptor isoform MOR-1 [Homo sapiens]
External Id: OPRM1-OPRD1_AG_LUMI_1536_1X%ACT PRUN
Name: QFRET-based biochemical primary high throughput screening assay to identify exosite i...
Source: The Scripps Research Institute Molecular Screening Center
Target: disintegrin and metalloproteinase domain-containing protein 17 preproprotein [Homo sapiens]
External Id: ADAM17_INH_QFRET_1536_1X%INH PRUN
Name: pKa (acid-base dissociation constant) as determined by Avdeef ref: DOI: 10.1002/04714...
Source: ChEMBL
Target: N/A
External Id: CHEMBL2449007
Name: Metabolic stability in rat liver homogenate assessed as compound hydrolysis at 10 uM ...
Source: ChEMBL
Target: Liver
External Id: CHEMBL3802103
Name: Fluorescence-based cell-based primary high throughput screening assay to identify ago...
Source: The Scripps Research Institute Molecular Screening Center
Target: muscarinic acetylcholine receptor M1 [Homo sapiens]
External Id: CHRM1_AG_FLUO8_1536_1X%ACT PRUN
Name: uHTS identification of small molecule activators of the adaptive arm of the Unfolded ...
Source: Burnham Center for Chemical Genomics
Target: N/A
External Id: BCCG-A405-UPR-XBP1-PrimaryAgonist-Assay
Name: Fluorescence polarization to screen for inhibitor that competite the binding of FadD2...
Source: Broad Institute
Target: FATTY-ACID-CoA LIGASE FADD28 (FATTY-ACID-CoA SYNTHETASE)
External Id: 2147-01_Inhibitor_SinglePoint_HTS_Activity
Name: Bursicon-induced LGR2 mediated cAMP production in LGR-2/CRE6x-Luciferase co-transfect...
Source: Broad Institute
Target: N/A
External Id: Bursicon-induced LGR2 mediated cAMP production in LGR-2/CRE6x-Luciferase co-transfected HEK293 cells Inhibition - 7011-01_Antagonist_SinglePoint_HTS_Activity
Name: Inhibition of human full-length cytosolic CA1 after 6 hrs by stopped flow CO2 hydrati...
Source: ChEMBL
Target: Carbonic anhydrase 1
External Id: CHEMBL1941821
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 Synonyms

7-hydroxy-4-methyl-8-nitro-chromen-2-one
4-methylumbelliferyl β-D-glucoside
4-Methyl-2-oxo-2H-chromen-7-yl β-D-glucopyranoside
4-methyl-7-hydroxy-8-nitro coumarin
EINECS 228-185-5
4-methyl-8-nitro-7-hydroxycoumarin
4-Methylumbelliferyl-beta-D-glucopyranoside
MFCD00036773
mutanolysin
7-Hydroxy-4-methyl-8-nitro-cumarin
(4-Methylumbelliferone)-β-D-glucopyranoside
7-hydroxy-4-methyl-8-nitro-coumarin
7-hydroxy-8-nitro-4-methyl-2H-chromen-2-one
4-Methylumbelliferyl β-D-glucopyranoside
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