Ac-Trp-Glu-His-Asp-AFC trifluoroacetate salt

Modify Date: 2024-01-16 14:50:27

Ac-Trp-Glu-His-Asp-AFC trifluoroacetate salt structure	Common Name	Ac-Trp-Glu-His-Asp-AFC trifluoroacetate salt
	CAS Number	210344-99-3	Molecular Weight	838.743
	Density	1.5±0.1 g/cm3	Boiling Point	1351.5±65.0 °C at 760 mmHg
	Molecular Formula	C₃₈H₃₇F₃N₈O₁₁	Melting Point	N/A
	MSDS	N/A	Flash Point	771.1±34.3 °C

Use of Ac-Trp-Glu-His-Asp-AFC trifluoroacetate salt

Ac-WEHD-AFC is a fluorogenic caspase-1 substrate. Ac-WEHD-AFC can measure caspase-1 fluorogenic activity and can be used for the research of tumor and inflammation[1].

Name	4-[[2-acetamido-3-(1H-indol-3-yl)propanoyl]amino]-5-[[1-[[3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid
Synonym	More Synonyms

Description	Ac-WEHD-AFC is a fluorogenic caspase-1 substrate. Ac-WEHD-AFC can measure caspase-1 fluorogenic activity and can be used for the research of tumor and inflammation[1].
Related Catalog	Research Areas >> Cancer Signaling Pathways >> Others >> Others Research Areas >> Inflammation/Immunology
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Caspase-1 Fluorogenic Activity Assay[1]: 1. Incubate the cells according to your normal protocol. 2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later. 3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays). 4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC. 5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute.

Density	1.5±0.1 g/cm3
Boiling Point	1351.5±65.0 °C at 760 mmHg
Molecular Formula	C₃₈H₃₇F₃N₈O₁₁
Molecular Weight	838.743
Flash Point	771.1±34.3 °C
Exact Mass	838.253418
PSA	312.23000
LogP	1.81
Vapour Pressure	0.0±0.3 mmHg at 25°C
Index of Refraction	1.648

AFC156

Ac-WEHD-AFC

N-Acetyl-L-tryptophyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]-L-α-asparagine

Ac-Trp-Glu-His-Asp-7-Amino-4-trifluoromethylcoumarin

L-α-Asparagine, N-acetyl-L-tryptophyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-

Ac-Trp-Glu-His-Asp-AFC

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Ac-Trp-Glu-His-Asp-AFC trifluoroacetate salt

Use of Ac-Trp-Glu-His-Asp-AFC trifluoroacetate salt

Names

Biological Activity

Chemical & Physical Properties

Synonyms

The content on this webpage is sourced from various professional data sources. If you have any questions or concerns regarding the content, please feel free to contact service1@chemsrc.com.