Ac-Trp-Glu-His-Asp-AFC trifluoroacetate salt
Modify Date: 2024-01-16 14:50:27
Ac-Trp-Glu-His-Asp-AFC trifluoroacetate salt structure
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Common Name | Ac-Trp-Glu-His-Asp-AFC trifluoroacetate salt | ||
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| CAS Number | 210344-99-3 | Molecular Weight | 838.743 | |
| Density | 1.5±0.1 g/cm3 | Boiling Point | 1351.5±65.0 °C at 760 mmHg | |
| Molecular Formula | C38H37F3N8O11 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | 771.1±34.3 °C | |
Use of Ac-Trp-Glu-His-Asp-AFC trifluoroacetate saltAc-WEHD-AFC is a fluorogenic caspase-1 substrate. Ac-WEHD-AFC can measure caspase-1 fluorogenic activity and can be used for the research of tumor and inflammation[1]. |
| Name | 4-[[2-acetamido-3-(1H-indol-3-yl)propanoyl]amino]-5-[[1-[[3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid |
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| Synonym | More Synonyms |
| Description | Ac-WEHD-AFC is a fluorogenic caspase-1 substrate. Ac-WEHD-AFC can measure caspase-1 fluorogenic activity and can be used for the research of tumor and inflammation[1]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Caspase-1 Fluorogenic Activity Assay[1]: 1. Incubate the cells according to your normal protocol. 2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later. 3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays). 4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC. 5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute. |
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 1351.5±65.0 °C at 760 mmHg |
| Molecular Formula | C38H37F3N8O11 |
| Molecular Weight | 838.743 |
| Flash Point | 771.1±34.3 °C |
| Exact Mass | 838.253418 |
| PSA | 312.23000 |
| LogP | 1.81 |
| Vapour Pressure | 0.0±0.3 mmHg at 25°C |
| Index of Refraction | 1.648 |
| AFC156 |
| Ac-WEHD-AFC |
| N-Acetyl-L-tryptophyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]-L-α-asparagine |
| Ac-Trp-Glu-His-Asp-7-Amino-4-trifluoromethylcoumarin |
| L-α-Asparagine, N-acetyl-L-tryptophyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]- |
| Ac-Trp-Glu-His-Asp-AFC |