SRPIN340
SRPIN340 structure
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Common Name | SRPIN340 | ||
|---|---|---|---|---|
| CAS Number | 218156-96-8 | Molecular Weight | 349.350 | |
| Density | 1.3±0.1 g/cm3 | Boiling Point | 395.9±42.0 °C at 760 mmHg | |
| Molecular Formula | C18H18F3N3O | Melting Point | N/A | |
| MSDS | USA | Flash Point | 193.3±27.9 °C | |
| Symbol |
GHS07 |
Signal Word | Warning | |
Use of SRPIN340SRPIN340 is an ATP-competitive serine-arginine-rich protein kinase (SRPK) inhibitor, with a Ki of 0.89 μM for SRPK1. |
| Name | N-[2-(1-Piperidinyl)-5-(trifluoromethyl)phenyl]isonicotinamide |
|---|---|
| Synonym | More Synonyms |
| Description | SRPIN340 is an ATP-competitive serine-arginine-rich protein kinase (SRPK) inhibitor, with a Ki of 0.89 μM for SRPK1. |
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| Related Catalog | |
| Target |
Ki: 0.89 μM (SRPK1)[1] |
| In Vitro | SRPIN340 is a serine-arginine-rich protein kinase (SRPK) inhibitor, with a Ki of 0.89 μM for SRPK1. SRPIN340 also inhibits SRPK2, but shows no significant inhibition on other SRPK, such as Clk1 and Clk4. SRPIN340 promotes degradation of SRp75, which is necessary for HIV expression. SRPIN340 suppresses the propagation of Sindbis virus (IC50, 60 μM) as well as severe acute respiratory syndrome virus[1]. SRPIN340 shows inhibitory effect on leukemia cell lines, such as AML HL60, ALL-T Molt4 and Jurkat, with IC50s of 44.7 μM, 92.2 μM and 82.3 μM, respectively[2]. |
| Cell Assay | Leukemic cells (5 × 104 cells/well) and isolated PBMCs (8 × 104 cells/well) are seeded in 96-well plates. Each well contained 100 μL of complete RPMI medium and 100 μL of SRPIN340 solution at different concentrations. The compound is diluted in RPMI medium with 10% fetal bovine serum and 0.4% DMSO (v/v). After 48 h of culture, MTT (5 mg/mL) is added to the wells (3 h, 37°C). The plates are centrifuged at room temperature for 30 min 500 ×g, followed by the removal of the MTT solution and the addition of 100 μL/well of DMSO to solubilize the formazan. Absorbance is measured at 540 nm in a microplate reader. Each experimental procedure is performed in triplicate[2]. |
| References |
| Density | 1.3±0.1 g/cm3 |
|---|---|
| Boiling Point | 395.9±42.0 °C at 760 mmHg |
| Molecular Formula | C18H18F3N3O |
| Molecular Weight | 349.350 |
| Flash Point | 193.3±27.9 °C |
| Exact Mass | 349.140198 |
| PSA | 45.23000 |
| LogP | 4.15 |
| Appearance of Characters | light yellow solid |
| Vapour Pressure | 0.0±0.9 mmHg at 25°C |
| Index of Refraction | 1.578 |
| Storage condition | -20℃ |
|
~85%
SRPIN340 CAS#:218156-96-8 |
| Literature: HAGIWARA, Masatoshi Patent: EP1712242 A1, 2006 ; Location in patent: Page/Page column 18; 31 ; |
| Precursor 2 | |
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| DownStream 0 | |
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Targeting SRPK1 to control VEGF-mediated tumour angiogenesis in metastatic melanoma.
Br. J. Cancer 111(3) , 477-85, (2014) Current therapies for metastatic melanoma are targeted either at cancer mutations driving growth (e.g., vemurafenib) or immune-based therapies (e.g., ipilimumab). Tumour progression also requires angi... |
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Specific inhibition of serine/arginine-rich protein kinase attenuates choroidal neovascularization.
Mol. Vis. 19 , 536-43, (2013) To investigate the applicability of serine/arginine-rich protein kinase (SRPK)-specific inhibitor, SRPIN340, for attenuation of choroidal neovascularization (CNV) formation using a mouse model.Laser p... |
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Dysregulation of splicing proteins in head and neck squamous cell carcinoma.
Cancer Biol. Ther. 17 , 219-29, (2016) Signaling plays an important role in regulating all cellular pathways. Altered signaling is one of the hallmarks of cancers. Phosphoproteomics enables interrogation of kinase mediated signaling pathwa... |
| 3,4-Dipropoxy-3-cyclobuten-1,2-dion |
| SR protein phosphorylation inhibitor 1 |
| 4-Pyridinecarboxamide (N-[2-(1-piperidinyl)-5-(trifluoromethyl)phenyl] |
| 4-Pyridinecarboxamide, N-[2-(1-piperidinyl)-5-(trifluoromethyl)phenyl]- |
| squaric acid dibutyl ester |
| N-[2-(1-Piperidinyl)-5-(trifluoromethyl)phenyl]isonicotinamide |
| 1,2-di-n-propoxy-cyclobutenedione |
| di-n-propyl squarate |
| (SRPIN)340 N-[2-(1-Piperidinyl)-5-(trifluoromethyl)phenyl]-4-pyridinecarboxamide |
| N4-[2-piperidino-5-(trifluoromethyl)phenyl]isonicotinamide |
| 3-Cyclobutene-1,2-dione,3,4-dipropoxy |
| SRPIN-340 |
| SRPIN340 |