TAK-779
TAK-779 structure
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Common Name | TAK-779 | ||
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| CAS Number | 229005-80-5 | Molecular Weight | 531.128 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C33H39ClN2O2 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of TAK-779TAK-779 is a potent and selective nonpeptide antagonist of CCR5 and CXCR3, with a Ki of 1.1 nM for CCR5, and effectively and selectively inhibits R5 HIV-1, with EC50 and EC90 of 1.2 nM and 5.7 nM, respectively, in MAGI-CCR5 cells. |
| Name | N,N-Dimethyl-N-[4-({[2-(4-methylphenyl)-6,7-dihydro-5H-benzo[7]an nulen-8-yl]carbonyl}amino)benzyl]tetrahydro-2H-pyran-4-aminium ch loride |
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| Synonym | More Synonyms |
| Description | TAK-779 is a potent and selective nonpeptide antagonist of CCR5 and CXCR3, with a Ki of 1.1 nM for CCR5, and effectively and selectively inhibits R5 HIV-1, with EC50 and EC90 of 1.2 nM and 5.7 nM, respectively, in MAGI-CCR5 cells. |
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| Related Catalog | |
| Target |
MIP-1α-CCR5:1 nM (IC50, in CHO/CCR5 cells) MIP-1β-CCR5:1 nM (IC50, in CHO/CCR5 cells) RANTES-CCR5:1.4 nM (IC50, in CHO/CCR5 cells) MCP-1-CCR2b:27 nM (IC50, in CHO/CCR5 cells) R5 HIV-1 (Ba-L):1.2 nM (EC50, in MAGI-CCR5 cells) R5 HIV-1 (KK):1.6 nM (EC50, in PBMCs) R5 HIV-1 (HHA):3.2 nM (EC50, in PBMCs) R5 HIV-1 (CTV):3.5 nM (EC50, in PBMCs) R5 HIV-1 (Ba-L):3.7 nM (EC50, in PBMCs) R5 HIV-1 (Ba-L):5.7 nM (EC90, in MAGI-CCR5 cells) R5 HIV-1 (HHA):7.5 nM (EC90, in PBMCs) R5 HIV-1 (Ba-L):12.8 nM (EC90, in PBMCs) R5 HIV-1 (KK):20.8 nM (EC90, in PBMCs) R5 HIV-1 (CTV):27 nM (EC90, in PBMCs) mCXCR3:369 nM (IC50, in PBMCs) |
| In Vitro | TAK-779 is a potent and selective nonpeptide antagonist of CCR5, with a Ki of 1.1 nM, and effectively and selectively inhibits R5 HIV-1, with EC50 and EC90 of 1.2 nM and 5.7 nM, respectively, in MAGI-CCR5 cells. TAK-779 less potently blocks the binding of [125I]-monocyte chemotactic protein 1 to CCR2b in CHO/CCR2b cells, with an IC50 for CCR2b of 27 nM. TAK-779 also completely inhibits the binding of [125I]-RANTES to CHO/CCR5 cells with an IC50 of 1.4 nM. TAK-779 (20 nM) selectively inhibits CCR5-mediated Ca2+-signaling. In addition, TAK-779 shows no inhibition on X4 HIV-1 strains[1]. TAK-779 is an antagonist of CXCR3, and inhibits the migration of T cells but not T cell proliferation[2]. |
| In Vivo | TAK-779 (10 mg/kg per day, s.c.) significantly prolongs the allograft survival of the rat intestinal transplantation model. TAK-779 also decreases the number of CD4+ as well as CD8+ T cells in spleen, blood and recipient mesenteric lymph nodes (MLN)[2]. TAK-779 (150 µg per mouse, s.c.) supppresses the development of experimental autoimmune encephalomyelitis (EAE) in myelin oligodendrocyte glycoprotein (MOG)-immunized C57BL/6 mice. TAK-779 decreases the infiltration of CXCR3 and CCR5 bearing leukocytes into the spinal cord. TAK-779 does not alter myelin oligodendrocyte glycoprotein (MOG)-specific immune responses or affect the potential of MOG-specific T cells to transfer experimental autoimmune encephalomyelitis (EAE)[3]. |
| Cell Assay | The anti-HIV-1 activities of the test compounds (TAK-779, etc.) are based on the inhibition of virus-induced infectious focus formation in MAGI-CCR5 cells and the reduction of p24 antigen production in PBMCs. In brief, MAGI-CCR5 cells (1 × 104 cells per well) are cultured in a microtiter tray. After a 24-h incubation at 37°C, the culture supernatants are replaced with fresh culture media containing the virus (≈300 focus forming units per well) and various concentrations of the test compounds (TAK-779, etc.). After a 2-day incubation, the cells are fixed and stained with 5-bromo-4-chloro-3-indolyl-β-d-galactosidase. The number of infected (blue) cells is counted microscopically. For the PBMC assays, phytohemagglutinin-stimulated PBMCs (2.5 × 105 cells per 500 μl) are infected with HIV-1 in the presence of various concentrations of the test compounds (TAK-779, etc.). The amounts of the virus used for infection are, depending on the replicability of each strain, generally 1-10 ng of p24 per 2.5 × 105 cells. After an overnight incubation at 37°C, the cells are washed extensively to remove unadsorbed viral particles and are incubated further with culture media containing the same concentrations of the compounds as those used during viral adsorption. On day 6 after viral infection, the culture supernatants are collected and determined for their p24 antigen levels with a sandwich ELISA kit. The cytotoxicities of the compounds are evaluated in parallel with their antiviral activities. They are based on the viability and proliferation of mock-infected cells[1]. |
| Animal Admin | Mice[3] The mice are immunized with MOG and are treated s.c. with TAK-779 or vehicle. The mice (N= 10) are injected s.c. with 150 µg TAK-779 (dissolved in 5% mannitol solution) in a volume of 100 µL, once daily after MOG immunization. TAK-779 injection is started from day 0 after immunization and continued once daily for 22 days. The dose of 150 µg is determined based on the observations in prior experiments that the dose of 50 µg per mouse can not produce inhibition, and a dose of more than 100 µg per mouse is required to produce significant inhibition. The dose of 150 µg per mouse has also been used in other mouse experimental models, and approximately the same dose is used in allograft rejection and asthma models. As a control, an equal volume of PBS containing 5% mannitol is injected daily in the control mice (N= 10)[3]. |
| References |
| Molecular Formula | C33H39ClN2O2 |
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| Molecular Weight | 531.128 |
| Exact Mass | 530.270020 |
| PSA | 26.30000 |
| LogP | 8.17110 |
| Storage condition | 2-8℃ |
| RIDADR | NONH for all modes of transport |
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Effects of a calcineurin inhibitor, FK506, and a CCR5/CXCR3 antagonist, TAK-779, in a rat small intestinal transplantation model.
Transpl. Immunol. 25(1) , 49-55, (2011) The effects of FK506, and TAK-779, antagonists of CCR5 and CXCR3, were investigated using a rat intestinal transplantation model. Small intestines from DA rats were heterotopically transplanted into L... |
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Evolution of CCR5 antagonist resistance in an HIV-1 subtype C clinical isolate.
J. Acquir. Immune Defic. Syndr. 55(4) , 420-7, (2010) We previously reported vicriviroc (VCV) resistance in an HIV-infected subject and used deep sequencing and clonal analyses to track the evolution of V3 sequence forms over 28 weeks of therapy. Here, w... |
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Synthesis of [19, 35, 36-(13)C(3)]-labeled TAK779 as a molecular probe.
Bioorg. Med. Chem. 17(16) , 5769-74, (2009) N,N-Dimethyl-N-[4-[[[2-(4-methylphenyl)-6,7-dihydro-5H-benzocyclohepten-8-yl]carbonyl]amino]benzyl]tetrahydro-2H-pyran-4-aminium chloride (TAK779) is a potent and selective non-peptide CCR5 antagonist... |
| N,N-Dimethyl-N-[4-({[2-(4-methylphenyl)-6,7-dihydro-5H-benzo[7]annulen-8-yl]carbonyl}amino)benzyl]tetrahydro-2H-pyran-4-aminium chloride |
| 2H-Pyran-4-aminium, N-[[4-[[[6,7-dihydro-2-(4-methylphenyl)-5H-benzocyclohepten-8-yl]carbonyl]amino]phenyl]methyl]tetrahydro-N,N-dimethyl-, chloride (1:1) |
| TAK-779 |