Crystal Violet

Modify Date: 2026-06-30 16:12:52

Crystal Violet structure	Common Name	Crystal Violet
	CAS Number	548-62-9	Molecular Weight	407.979
	Density	1.19 g/cm3 (20ºC)	Boiling Point	N/A
	Molecular Formula	C₂₅H₃₀ClN₃	Melting Point	215ºC
	MSDS	Chinese USA	Flash Point	40ºC
	Symbol	GHS02, GHS07, GHS08, GHS09	Signal Word	Warning

Use of Crystal Violet

Crystal violet is a triarylmethane dye.Target: OthersCrystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. The compound helps to access bacterial contamination of tissue culture samples. The staining response is due to the difference in the chemical and structural composition of the cell walls in different bacteria. Crystal Violet can also be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines [1-3].

Summary: Crystal Violet (CAS number: 548-62-9), with molecular formula C25H30ClN3 and molecular weight 407.979, appears as dark green powder or crystals, with a melting point of 215°C and a density of 1.19 g/cm³. It is mainly used for manufacturing ink, carbon paper, gentian violet, and dyeing. It is also used for staining and producing pigments. For research-use only, not for medical or clinical usage.

This table lists Chinese names, IUPAC names and various aliases of this chemical substance.

Name	crystal violet
Synonym	More Synonyms

This table contains bioactivity, target information and biological‑assay experimental data of this compound.

Description	Crystal violet is a triarylmethane dye.Target: OthersCrystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. The compound helps to access bacterial contamination of tissue culture samples. The staining response is due to the difference in the chemical and structural composition of the cell walls in different bacteria. Crystal Violet can also be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines [1-3].
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
References	[1]. Beveridge, T.J. and S. Schultze-Lam, The response of selected members of the archaea to the gram stain. Microbiology, 1996. 142 ( Pt 10): p. 2887-95. [2]. Coico, R., Gram staining. Curr Protoc Microbiol, 2005. Appendix 3: p. Appendix 3C. [3]. Bil, J., et al., Statins potentiate cytostatic/cytotoxic activity of sorafenib but not sunitinib against tumor cell lines in vitro. Cancer Lett, 2010. 288(1): p. 57-67.

Description

Related Catalog

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

References

[1]. Beveridge, T.J. and S. Schultze-Lam, The response of selected members of the archaea to the gram stain. Microbiology, 1996. 142 ( Pt 10): p. 2887-95.

[2]. Coico, R., Gram staining. Curr Protoc Microbiol, 2005. Appendix 3: p. Appendix 3C.

[3]. Bil, J., et al., Statins potentiate cytostatic/cytotoxic activity of sorafenib but not sunitinib against tumor cell lines in vitro. Cancer Lett, 2010. 288(1): p. 57-67.

This table shows physicochemical parameters including melting point, boiling point, density, solubility and optical rotation. Missing data is marked as N/A.

Density	1.19 g/cm3 (20ºC)
Melting Point	215ºC
Molecular Formula	C₂₅H₃₀ClN₃
Molecular Weight	407.979
Flash Point	40ºC
Exact Mass	407.212830
PSA	9.49000
LogP	1.46360
InChIKey	ZXJXZNDDNMQXFV-UHFFFAOYSA-M
SMILES	CN(C)c1ccc(C(=C2C=CC(=[N+](C)C)C=C2)c2ccc(N(C)C)cc2)cc1.[Cl-]

This table provides MSDS information including hazard classification, first‑aid, fire‑fighting, spill handling, operation and storage instructions.

Crystal Violet MSDS(Chinese)

This table summarizes toxicological test data, acute & chronic toxicity and ecological hazard parameters for this chemical.

CHEMICAL IDENTIFICATION

RTECS NUMBER :: BO9000000

CHEMICAL NAME :: Ammonium, (4-(bis(p-(dimethylamino)phenyl)methylene)-2,5-cycloh exadien-1-ylidene) dimethyl-, chloride

CAS REGISTRY NUMBER :: 548-62-9

LAST UPDATED :: 199710

DATA ITEMS CITED :: 48

MOLECULAR FORMULA :: C25-H30-N3.Cl

MOLECULAR WEIGHT :: 408.03

WISWESSER LINE NOTATION :: L6Y DYJ AUYR DN1&1&R DN1&1& DUK1&1 &Q &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: Standard Draize test

ROUTE OF EXPOSURE :: Administration onto the skin

SPECIES OBSERVED :: Human

TYPE OF TEST :: Standard Draize test

ROUTE OF EXPOSURE :: Administration onto the skin

SPECIES OBSERVED :: Human

TYPE OF TEST :: Standard Draize test

ROUTE OF EXPOSURE :: Administration onto the skin

SPECIES OBSERVED :: Rodent - guinea pig

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 420 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 8900 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 96 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 5100 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 20 mg/kg

TOXIC EFFECTS :: Lungs, Thorax, or Respiration - acute pulmonary edema

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Mammal - dog

DOSE/DURATION :: 1 gm/kg

TOXIC EFFECTS :: Behavioral - food intake (animal) Gastrointestinal - nausea or vomiting Nutritional and Gross Metabolic - weight loss or decreased weight gain

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Mammal - cat

DOSE/DURATION :: 100 mg/kg

TOXIC EFFECTS :: Gastrointestinal - hypermotility, diarrhea Gastrointestinal - nausea or vomiting Nutritional and Gross Metabolic - weight loss or decreased weight gain

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 150 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 5 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraduodenal

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 160 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - guinea pig

DOSE/DURATION :: 100 mg/kg

TOXIC EFFECTS :: Behavioral - ataxia

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - guinea pig

DOSE/DURATION :: 10 mg/kg

TOXIC EFFECTS :: Lungs, Thorax, or Respiration - acute pulmonary edema

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 4 mg/kg/2D-I

TOXIC EFFECTS :: Gastrointestinal - peritonitis Related to Chronic Data - death

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 10950 mg/kg/2Y-C

TOXIC EFFECTS :: Tumorigenic - Carcinogenic by RTECS criteria Endocrine - thyroid tumors

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 25750 mg/kg/2Y-C

TOXIC EFFECTS :: Tumorigenic - Carcinogenic by RTECS criteria Kidney, Ureter, Bladder - tumors Reproductive - Tumorigenic effects - ovarian tumors

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 100 mg/kg

SEX/DURATION :: female 6-15 day(s) after conception

TOXIC EFFECTS :: Reproductive - Specific Developmental Abnormalities - musculoskeletal system Reproductive - Specific Developmental Abnormalities - urogenital system

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 40 mg/kg

SEX/DURATION :: female 6-15 day(s) after conception

TOXIC EFFECTS :: Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants)

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 100 mg/kg

SEX/DURATION :: female 6-15 day(s) after conception

TOXIC EFFECTS :: Reproductive - Specific Developmental Abnormalities - other developmental abnormalities

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 7 mg/kg

SEX/DURATION :: female 6-19 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus)

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 7 mg/kg

SEX/DURATION :: female 6-19 day(s) after conception

TOXIC EFFECTS :: Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants) Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus)

MUTATION DATA

TYPE OF TEST :: Cytogenetic analysis

TEST SYSTEM :: Non-mammalian species Cells - not otherwise specified

DOSE/DURATION :: 500 ug/L

REFERENCE :: MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 58,269,1978 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M1517 No. of Facilities: 9858 (estimated) No. of Industries: 58 No. of Occupations: 52 No. of Employees: 79824 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M1517 No. of Facilities: 3280 (estimated) No. of Industries: 21 No. of Occupations: 34 No. of Employees: 75632 (estimated) No. of Female Employees: 49319 (estimated)

This table covers safety warnings, protective measures, incompatible substances and disposal guidelines for this compound.

Symbol	GHS02, GHS07, GHS08, GHS09
Signal Word	Warning
Hazard Statements	H226-H319-H351-H411
Precautionary Statements	P273-P281-P305 + P351 + P338
Personal Protective Equipment	Eyeshields;Faceshields;full-face respirator (US);Gloves;multi-purpose combination respirator cartridge (US);type ABEK (EN14387) respirator filter
Hazard Codes	T:Toxic
Risk Phrases	R22;R41;R45;R50/53
Safety Phrases	S45-S53-S60-S61
RIDADR	UN 3077
WGK Germany	3
RTECS	BO9000000
Packaging Group	III
Hazard Class	9
HS Code	32041300

This table presents publicly reported synthetic routes, reaction conditions, reactants and product information of the compound.

This table lists upstream starting materials and downstream derivative products related to this chemical.

Precursor 9
CAS#:121-69-7 N,N-Dimethylaniline CAS#:100-10-7 p-Dimethylamino... CAS#:603-48-5 Leucocrystal violet CAS#:90-94-8 Michler's ketone
CAS#:75-44-5 phosgene CAS#:56-23-5 Carbon tetrachloride CAS#:467-63-0 Solvent Violet 9 CAS#:503-38-8 Diphosgene
CAS#:79-37-8 Oxalyl chloride
DownStream 10
CAS#:619-60-3 4-(Dimethylamin... CAS#:90-94-8 Michler's ketone CAS#:121-69-7 N,N-Dimethylaniline CAS#:603-48-5 Leucocrystal violet
CAS#:101-61-1 4,4'-Methyleneb... CAS#:328010-96-4 1,1,1-tris-(4-n... CAS#:93522-25-9 4-[1,1-bis[4-(d... CAS#:4439-06-9 Crystal Violet ...
CAS#:596-49-6 p,p',p''-tris(d... CAS#:467-63-0 Solvent Violet 9

This table shows customs‑related data including HS‑code, tariff and regulatory conditions for import and export.

HS Code	32041300

This table lists relevant public references with title, journal and publication metadata for this compound.

Single nucleotide polymorphism analysis of the major tripartite multidrug efflux pump of Escherichia coli: functional conservation in disparate animal reservoirs despite exposure to antimicrobial chemotherapy.

Antimicrob. Agents Chemother. 54 , 1007-15, (2010)

AcrAB-TolC imparts a strong intrinsic resistance phenotype to many clinically significant molecules in Escherichia coli. This complex is composed of a pump, AcrB, and a periplasmic protein, AcrA, that...

Biologic activities of the TolC-like protein of Neisseria meningitidis as assessed by functional complementation in Escherichia coli.

Antimicrob. Agents Chemother. 54 , 506-8, (2010)

Neisseria meningitidis can produce a TolC-like protein needed for secretion of FrpC but not efflux of antimicrobials. We now report that expression of the meningococcal tolC gene in a TolC-deficient s...

Characterization of TolC efflux pump proteins from Pasteurella multocida.

Antimicrob. Agents Chemother. 52 , 4166-71, (2008)

Two TolC homologs, PM0527 and PM1980, were identified for Pasteurella multocida. A pm0527 mutant displayed increased susceptibility to a range of chemicals, including rifampin (512-fold) and acridine ...

This table contains target information, in‑vitro & in‑vivo assay data for this compound.

Name: Primary qHTS assay for inhibitors of alpha-synuclein gene (SNCA) expression
Source: NCGC
External Id: SNCA-p-activity-luciferase

Name: Cell Viability Profiling of Human Lymphoblast Cell Lines
Source: NCGC
Target: N/A
External Id: HAPV101

Name: Caspase-3/7 Activation Profiling of Human Lymphoblast Cell Lines
Source: NCGC
Target: N/A
External Id: HAPC101

Name: Cytochrome P450 Family 1 Subfamily A Member 2 (CYP1A2) small molecule antagonists: lu...
Source: 824
External Id: CYP273

Name: Increase the activity of the Burkholderia fixLJ 2-component system
Source: ICCB-Longwood/NSRB Screening Facility, Harvard Medical School
Target: Burkholderia multivorans
External Id: HMS1625

Name: Trypanocidal activity against Trypanosoma cruzi Tulahuen epimastigotes assessed as mi...
Source: ChEMBL
Target: Trypanosoma cruzi
External Id: CHEMBL1015624

Name: qHTS Assay for Small Molecule Inhibitors of the Human hERG Channel Activity
Source: NCGC
External Id: HERG01

Name: Antiparasitic activity against bloodstream trypomastigote stage of Trypanosoma cruzi ...
Source: ChEMBL
Target: Trypanosoma cruzi
External Id: CHEMBL3631891

Name: Cytotoxicity in LPS-stimulated mouse RAW264.7 cells assessed as reduction in cell via...
Source: ChEMBL
Target: RAW264.7
External Id: CHEMBL5130483

Name: Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in IFN-gamma a...
Source: ChEMBL
Target: RAW264.7
External Id: CHEMBL5130484

Total 626, Current Page 1 of 63

This table collects all English aliases, systematic names and CAS‑related synonyms of the compound.

Crystal violet

Basic Violet 3

EINECS 208-953-6

Methyl violet

MFCD00011750

This section contains frequently‑asked‑questions about this compound, including basic parameters, properties, storage conditions and corresponding answers.

Q: What is the safety information for crystal violet?

A: Safety information for crystal violet: S45-S53-S60-S61.

Q: What is the CAS number of crystal violet?

A: CAS number of crystal violet is 548-62-9.

Q: What is the English name of crystal violet?

A: The English name of crystal violet is crystal violet.

Q: What are the upstream materials of crystal violet?

A: Related upstream materials(CAS) of crystal violet: 121-69-7;100-10-7;603-48-5;90-94-8;75-44-5;56-23-5;467-63-0;503-38-8;79-37-8.

Q: What is the melting point of crystal violet?

A: Melting point of crystal violet is 215ºC.

Q: What is the LogP of crystal violet?

A: LogP of crystal violet is 1.46360.

Q: What is the molecular formula of crystal violet?

A: Molecular formula of crystal violet is C25H30ClN3.

Q: What is the SMILES notation of crystal violet?

A: SMILES notation of crystal violet is CN(C)c1ccc(C(=C2C=CC(=[N+](C)C)C=C2)c2ccc(N(C)C)cc2)cc1.[Cl-].

Q: What is the polar surface area (PSA) of crystal violet?

A: Polar surface area (PSA) of crystal violet is 9.49000.

Q: What are the downstream products of crystal violet?

A: Related downstream products(CAS) of crystal violet: 619-60-3;90-94-8;121-69-7;603-48-5;101-61-1;328010-96-4;93522-25-9;4439-06-9;596-49-6;467-63-0.

This table lists manufacturers and suppliers of this compound, including vendor names and product supply reference information.

Shanghai Nianxing Industrial Co., Ltd

Dayang Chem (Hangzhou) Co., Ltd.

Henan Tianfu Chemical Co., Ltd.

The content on this webpage is sourced from various professional data sources. If you have any questions or concerns regarding the content, please feel free to contact service1@chemsrc.com.

Shanghai Nianxing Industrial Co., Ltd
China
Product Name: Crystal Violet
Price: Check
Purity: 98.0%
Delivery: 10 Day
Contact: Yang
Email: sales@echemcloud.com

Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: Basic Violet 3
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
Delivery: Inquiry
Contact: Ms Wang
Email: enquiry@dycnchem.com

Henan Tianfu Chemical Co., Ltd.
China
Product Name: Crystal Violet
Price: $Inquiry/1kg $Inquiry/25kg $Inquiry/1000kg $Inquiry/10ton
Purity: 99.0%
Delivery: Inquiry
Contact: Anson
Email: info@tianfuchem.com

Get all suppliers and price by the below link:

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Price: $60/5g

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Crystal Violet

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Crystal Violet Biological Activity

Chemical & Physical Properties

MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

Safety Information

Synthetic Route

Precursor & DownStream

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