LY-294,002 hydrochloride

Modify Date: 2024-01-11 17:44:07

LY-294,002 hydrochloride structure	Common Name	LY-294,002 hydrochloride
	CAS Number	934389-88-5	Molecular Weight	343.80400
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₁₉H₁₈ClNO₃	Melting Point	N/A
	MSDS	Chinese USA	Flash Point	N/A

Use of LY-294,002 hydrochloride

LY294002 hydrochloride is a potent and broad-spectrum PI3K inhibitor, with IC50 values of 0.5, 0.57, and 0.97 μM for P110α, P110δ and P110β, respectively. LY294002 hydrochloride also inhibits CK2 with an IC50 of 98 nM. LY294002 hydrochloride can be used for pancreatic cancer research[1][2][3].

Name	2-morpholin-4-yl-8-phenylchromen-4-one,hydrochloride
Synonym	More Synonyms

Description	LY294002 hydrochloride is a potent and broad-spectrum PI3K inhibitor, with IC50 values of 0.5, 0.57, and 0.97 μM for P110α, P110δ and P110β, respectively. LY294002 hydrochloride also inhibits CK2 with an IC50 of 98 nM. LY294002 hydrochloride can be used for pancreatic cancer research[1][2][3].
Related Catalog	Signaling Pathways >> Apoptosis >> Apoptosis Research Areas >> Cancer Signaling Pathways >> Cell Cycle/DNA Damage >> DNA-PK Signaling Pathways >> PI3K/Akt/mTOR >> PI3K Signaling Pathways >> Cell Cycle/DNA Damage >> Casein Kinase Signaling Pathways >> PI3K/Akt/mTOR >> DNA-PK Signaling Pathways >> Stem Cell/Wnt >> Casein Kinase
Target	CK2:0.098 μM (IC50) CK2α2:3.869 μM (IC50)
In Vitro	LY294002 hydrochloride (0-75 μM; 24 hours and 48 hours) remarkably decreases human nasopharyngeal carcinoma CNE-2Z cells in a dose-dependent fashion[4]. LY294002 hydrochloride (0-75 μM; 24 hours and 48 hours ) induces CNE-2Z cells apoptosis rate in dose-dependent[4]. LY294002 hydrochloride (10-75 μM) significantly decreases p-Akt (S473) expression levels and up-regulates caspase-9 activity in CNE-2Z cells. Total Akt protein level is not difference with different concentration[4]. LY294002 hydrochloride (5, 10, 100 µM; for 2 hours) treatment partially suppresses Lysophosphatidic acid (LPA)-induced (20 µM; for 4 hours) nuclear translocation of YAP, accompanied by a reduction in p-AKT levels[6]. Cell Proliferation Assay Cell Line: CNE-2Z cells[4] Concentration: 0 μM, 10 μM, 25 μM, 50 μM, and 75 μM Incubation Time: 24 hours and 48 hours Result: Decreased CNE-2Z cells in a dose-dependent fashion. Apoptosis Analysis Cell Line: CNE-2Z cells[4] Concentration: 0 μM, 10 μM, 25 μM, 50 μM, and 75 μM Incubation Time: 24 hours and 48 hours Result: Induced apoptosis rate in a dose-dependent manner. Western Blot Analysis Cell Line: CNE-2Z cells[4] Concentration: 0 μM, 10 μM, 25 μM, 50 μM, and 75 μM Incubation Time: 24 hours and 48 hours Result: Decreased phosphorylated Akt (S473) expression levels were significantly, up-regulated caspase-9 activity in CNE-2Z cells in treated group.
In Vivo	LY294002 hydrochloride (10, 25, 50, 75 mg/kg; i.p.; twice weekly; for 4 weeks) significantly reduces mean NPC tumor burden in a dose-dependent manner. LY294002 (10, 25 mg/kg) is less effective in decreasing tumor burden[4]. LY294002 hydrochloride (1.2 mg/kg per day; i.p.; for 14 days) prevents Leptin (60 ug/kg)-induced adverse effects on spermatozoa in Sprague-Dawley rats[5]. Animal Model: Athymic nude mice (6-8 weeks) with CNE-2Z xenograft[4] Dosage: 10 mg/kg, 25 mg/kg, 50 mg/kg, and 75 mg/kg Administration: IP; twice weekly, for 4 weeks Result: Mean Nasopharyngeal carcinoma (NPC) tumor burden was remarkably decreased in a dose-dependent manner.
References	[1]. Bai R, et al. The effect of PI3K inhibitor LY294002 and gemcitabine hydrochloride combined with ionizing radiation on the formation of vasculogenic mimicry of Panc-1 cells in vitro and in vivo. Neoplasma. 2016;63(1):80-92. [2]. Chaussade C, et al. Evidence for functional redundancy of class IA PI3K isoforms in insulin signalling. Biochem J. 2007 Jun 15;404(3):449-58. [3]. Gharbi SI, et al. Exploring the specificity of the PI3K family inhibitor LY294002. Biochem J. 2007 May 15;404(1):15-21. [4]. Jiang H, et al. Phosphatidylinositol 3-kinase inhibitor(LY294002) induces apoptosis of human nasopharyngeal carcinoma invitro and in vivo. J Exp Clin Cancer Res. 2010 Apr 22;29:34. [5]. Md Mokhtar AH, et al. LY294002, a PI3K pathway inhibitor, prevents leptin-induced adverse effects on spermatozoa in Sprague-Dawley rats. Andrologia. 2019 Apr;51(3):e13196. [6]. Yi-Jen Hsueh, et al. Lysophosphatidic acid induces YAP-promoted proliferation of human corneal endothelial cells via PI3K and ROCK pathways. Mol Ther Methods Clin Dev. 2015 Apr 29;2:15014.

Molecular Formula	C₁₉H₁₈ClNO₃
Molecular Weight	343.80400
Exact Mass	343.09800
PSA	42.68000
LogP	4.16360
Storage condition	2-8°C

LY-294,002 hydrochloride MSDS(Chinese)

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR	NONH for all modes of transport

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LY-294,002 hydrochloride

LY-294,002 hydrochloride

Use of LY-294,002 hydrochloride

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