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4,5-二氨基荧光素二乙酸酯

4,5-二氨基荧光素二乙酸酯用途

DAF 2DA 是测量 NO 最广泛使用的一种荧光探针。

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4,5-二氨基荧光素二乙酸酯名称

[ CAS 号 ]:
205391-02-2

[ 中文名 ]:
4,5-二氨基荧光素二乙酸酯

[ 英文名 ]:
4,5-Diaminofluorescein diacetate

[英文别名 ]:

5,6-Diaminofluorescein diacetate
DAF 2DA

4,5-二氨基荧光素二乙酸酯生物活性

[ 描述 ]:

DAF 2DA 是测量 NO 最广泛使用的一种荧光探针。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

研究领域 >> 其他

染料试剂

[体外研究]

一氧化氮是自由基信号分子。有多种方法可用于测量NO。在所有方法中,用于定位NO的荧光探针是非常广泛使用的方法。二乙酸盐形式的二氨基荧光素(DAF 2DA)是用于NO测量的最广泛的探针。该方法基于4,5-二氨基荧光素二乙酸酯(DAF 2DA)的应用,其活跃地扩散到细胞中,一旦被细胞吸收,细胞质酯酶切割乙酸酯基团以产生4,5-二氨基荧光素; DAF-2。产生的DAF-2可以容易地与N 2 O 3反应,N 2 O 3是NO的氧化产物,以产生高度荧光的DAF-2T(三唑基uorescein)。

[参考文献]

[1]. Wany A, et al. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence. Methods Mol Biol. 2016;1424:39-47.

[相关活性小分子]

4,5-二氨基荧光素二乙酸酯物理化学性质

[ 密度 ]:
1.5±0.1 g/cm3

[ 沸点 ]:
719.0±60.0 °C at 760 mmHg

[ 分子式 ]:
C₂₄H₁₈N₂O₇

[ 分子量 ]:
446.409

[ 闪点 ]:
320.0±29.2 °C

[ 精确质量 ]:
446.111389

[ PSA ]:
140.17000

[ LogP ]:
1.20

[ 蒸汽压 ]:
0.0±2.3 mmHg at 25°C

[ 折射率 ]:
1.721

[ 储存条件 ]:
2-8℃

4,5-二氨基荧光素二乙酸酯MSDS

详细MSDS(安全技术说明书)

4,5-二氨基荧光素二乙酸酯安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 风险声明 (欧洲) ]:
R36/38

[ 安全声明 (欧洲) ]:
26

[ 危险品运输编码 ]:
NONH for all modes of transport

4,5-二氨基荧光素二乙酸酯文献

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