β-鹅膏菌素

β-鹅膏菌素用途

β-Amanitin 是毒蘑菇中的一种环肽毒素。β-Amanitin 抑制真核RNA聚合酶 II 和 III (RNA polymerase II/III)。β-Amanitin 抑制蛋白质合成。β-Amanitin 可以用来作为抗体偶联药物 (ADCs) 的细胞毒性成分。

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β-鹅膏菌素名称

[ CAS 号 ]:
21150-22-1

[ 中文名 ]:
β-鹅膏菌素

[ 英文名 ]:
beta-Amanitin

[英文别名 ]:

MFCD00151212
EINECS 244-244-8
B-amanitin from amanita phalloides
B-Amanitine

β-鹅膏菌素生物活性

[ 描述 ]:

[ 相关类别 ]:

研究领域 >> 癌症

信号通路 >> 细胞周期/DNA损伤 >> DNA/RNA合成

信号通路 >> 抗体- 药物偶联物 >> ADC细胞毒素

[ 靶点 ]

Traditional Cytotoxic Agents

[体外研究]

β-鹅膏素(0.01-100微克/毫升；36小时)对MCF-7细胞具有毒性,在100、10、1、0.1和0.01微克/毫升浓度下,细胞存活率分别为52%、62%、84%、86%和91%[2]。β-鹅膏素对MCF-7细胞内蛋白质合成的抑制作用在两种浓度(10μg/mL和1μg/mL)下均达24小时[2]。细胞活力测定[2]细胞系：MCF-7细胞浓度：0.01、0.1、1、10、100微克/毫升培养时间：36小时结果：MCF-7细胞呈毒性反应。

[参考文献]

[1]. Kaya E, et al. Evaluation and comparison of alpha- and beta-amanitin toxicity on MCF-7 cell line. Turk J Med Sci. 2014;44(5):728-32.

[2]. Lutz C, et al. Alpha- and Beta-Amanitin Total Synthesis. Angew Chem Int Ed Engl. 2020 Feb 24.

β-鹅膏菌素物理化学性质

[ 分子式 ]:
C₃₉H₅₃N₉O₁₅S

[ 分子量 ]:
919.95400

[ 精确质量 ]:
919.33800

[ PSA ]:
394.30000

[ 折射率 ]:
1.695

[ 储存条件 ]:
库房通风低温干燥

β-鹅膏菌素MSDS

详细MSDS(安全技术说明书)

β-鹅膏菌素毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: NJ8324000

CAS REGISTRY NUMBER :: 21150-22-1

LAST UPDATED :: 199612

DATA ITEMS CITED :: 2

MOLECULAR FORMULA :: C39-H53-N9-O15-S

MOLECULAR WEIGHT :: 920.07

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 400 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: NEJMAG New England Journal of Medicine. (Massachusetts Medical Soc., 10 Shattuck St., Boston, MA 02115) V.198- 1928- Volume(issue)/page/year: 269,223,1963

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Unreported

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 400 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag, Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951- Volume(issue)/page/year: 22,2142,1972

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β-鹅膏菌素安全信息

[ 符号 ]:

GHS06

[ 信号词 ]:
Danger

[ 危害声明 ]:
H300-H310-H330

[ 警示性声明 ]:
P260-P264-P280-P284-P302 + P350-P310

[ 危害码 (欧洲) ]:
T+

[ 风险声明 (欧洲) ]:
R26/27/28

[ 安全声明 (欧洲) ]:
S7;S28;S45;S36/S37/S39

[ 危险品运输编码 ]:
UN 3462 6.1/PG 1

[ RTECS号 ]:
NJ8324000

[ 危险类别 ]:
6.1(a)

β-鹅膏菌素文献

[Determination of amanitatoxins by HPLC].

Chudoku. Kenkyu. 16(4) , 441-5, (2003)

High-performance liquid chromatographic (HPLC) assay has been developed for the simultaneous determination of alpha-amanitin, beta-amanitin and phalloidin in serum. Three toxins were extracted by refl...

Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) in toxicological analysis. Studies on the detection of clobenzorex and its metabolites within a systematic toxicological analysis procedure by GC-MS and by immunoassay and studies on the detection of alpha- and beta-amanitin in urine by atmospheric pressure ionization electrospray LC-MS.

J. Chromatogr. B. Biomed. Sci. Appl. 689(1) , 81-9, (1997)

GC-MS is the method of choice for toxicological analysis of toxicants volatile in GC while non-volatile and/or thermally labile toxicants need LC-MS for their determination. Studies are presented on t...

Validated electrospray liquid chromatographic-mass spectrometric assay for the determination of the mushroom toxins alpha- and beta-amanitin in urine after immunoaffinity extraction.

J. Chromatogr. B. Biomed. Sci. Appl. 748(1) , 125-35, (2000)

Specific detection of amanitins in body fluids is necessary for an early diagnosis of an intoxication with amanita mushrooms. In this paper, a liquid chromatographic-mass spectrometric assay after imm...

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