2'-岩藻糖基乳糖

2'-岩藻糖基乳糖用途

2'-岩藻糖基乳糖(2'-FL)是一种可以从母乳中提取的低聚糖。2'-岩藻糖基乳糖调节CD14的表达,缓解结肠炎并调节肠道微生物。2'-岩藻糖基乳糖刺激T细胞增加IFN-γ的生成,并降低IL-6、IL-17和TNF-α的细胞因子生成[1][2]。

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2'-岩藻糖基乳糖名称

[ CAS 号 ]:
41263-94-9

[ 中文名 ]:
2'-岩藻糖基乳糖

[ 英文名 ]:
2'-Fucosyllactose

[英文别名 ]:

2'-岩藻糖基乳糖生物活性

[ 描述 ]:

[ 相关类别 ]:

研究领域 >> 感染

信号通路 >> 免疫及炎症 >> 白细胞介素相关

研究领域 >> 炎症/免疫

[ 靶点 ]

Target: IFN-γ, IL-6, IL-17, and TNF-α[1]

[体外研究]

2'-岩藻糖基乳糖(2'-FL；0-12 mg/mL；48 h)抑制T84细胞中细胞相关CD14表达,并减弱LPS(100μg/mL)刺激的IL-8分泌[1]。2'-岩藻糖基乳糖(2 mg/mL；48 h；T84和HCT8细胞)可改善细菌入侵引起的炎症。2'-岩藻糖基乳糖抑制ETEC侵袭并减弱随后的IL-8分泌[1]。2'-岩藻糖基乳糖(2 mg/mL；48 h；T84和HCT8细胞)诱导巨噬细胞移动抑制因子信号通路,抑制炎症[1]。Western Blot分析[1]细胞系：T84细胞浓度：0、2和4 mg/mL培养时间：48小时结果：抑制CD14 mRNA并降低细胞相关CD14蛋白表达。Western Blot分析[1]细胞系：T84和HCT8细胞T84和HCT8细胞浓度：2 mg/mL培养时间：48小时结果：抑制CD14 mRNA并降低细胞相关CD14蛋白表达。抑制CD14和NF-κB的诱导。诱导iκB和SOCS2表达和STAT3磷酸化。

[体内研究]

2'-岩藻糖基乳糖(2'-FL；100 mg(200μL)；i、例如。；每日,连续4d；感染AIEC的C57BL/6小鼠)抑制体内AIEC感染和炎症[1]。动物模型：AIEC感染C57BL/6小鼠(8周)[1]剂量：100 mg(200μL)口服；结果：结肠长度接近正常值。抑制O83阳性细菌对结肠粘膜的定植。结肠中CD14表达、CD14 mRNA水平、IL-6、IL-17和TNF-α水平降低。

[参考文献]

[1]. He Y, et, al. The human milk oligosaccharide 2'-fucosyllactose modulates CD14 expression in human enterocytes, thereby attenuating LPS-induced inflammation. Gut. 2016 Jan;65(1):33-46.

[2]. Eiwegger T, et, al. Human milk--derived oligosaccharides and plant-derived oligosaccharides stimulate cytokine production of cord blood T-cells in vitro. Pediatr Res. 2004 Oct;56(4):536-40.

2'-岩藻糖基乳糖物理化学性质

[ 密度 ]:
1.68g/cm3

[ 沸点 ]:
902.2ºC at 760 mmHg

[ 熔点 ]:
230-231 °C

[ 分子式 ]:
C₁₈H₃₂O₁₅

[ 分子量 ]:
488.43800

[ 闪点 ]:
311.9ºC

[ 精确质量 ]:
488.17400

[ PSA ]:
248.45000

[ 外观性状 ]:
固体

[ 蒸汽压 ]:
0mmHg at 25°C

[ 折射率 ]:
1.631

[ 储存条件 ]:
-20°C，密封，惰性气体

2'-岩藻糖基乳糖安全信息

[ 危害码 (欧洲) ]:
Xi

[ 安全声明 (欧洲) ]:
24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

2'-岩藻糖基乳糖文献

[Studies of oligosaccharide specificity of perch roe fucolectin, using gold labeled neoglycoproteins].

Prikl. Biokhim. Mikrobiol. 39 , 105-109, (2003)

The specificity of perch (Perca fluviatilis) roe fucolectin was studied using the protein dot blot technique, followed by detection with colloidal gold-labeled neoglycoproteins bearing human milk poly...

Human milk oligosaccharides influence maturation of human intestinal Caco-2Bbe and HT-29 cell lines.

J. Nutr. 144(5) , 586-91, (2014)

Stimulation of gastrointestinal tract maturation is 1 of the many benefits of human milk. Human milk oligosaccharides (HMOs) are abundant in human milk and are reported to promote enterocyte different...

Lacto-N-tetraose, fucosylation, and secretor status are highly variable in human milk oligosaccharides from women delivering preterm.

J. Proteome Res. 11(9) , 4662-72, (2012)

Breast milk is the ideal nutrition for term infants but must be supplemented to provide adequate growth for most premature infants. Human milk oligosaccharides (HMOs) are remarkably abundant and diver...

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