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双(1,3-二巴比妥酸)-三次甲基氧烯洛尔

双(1,3-二巴比妥酸)-三次甲基氧烯洛尔用途

DiBAC4(3) 是一种电压敏感的荧光染料 (λex=490 nm,λem=505 nm)。

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双(1,3-二巴比妥酸)-三次甲基氧烯洛尔名称

[ CAS 号 ]:
70363-83-6

[ 中文名 ]:
双(1,3-二丁基巴比妥酸)三次甲基氧杂菁

[ 英文名 ]:
DiSBAC2(5)

[中文别名 ]:

双(1,3-二巴比妥酸)-三次甲基氧烯洛尔

[英文别名 ]:

dibac4(3)
MFCD00467633

双(1,3-二巴比妥酸)-三次甲基氧烯洛尔生物活性

[ 描述 ]:

DiBAC4(3) 是一种电压敏感的荧光染料 (λex=490 nm,λem=505 nm)。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

用DiBAC4(3)清楚地观察到HEKBKα中10μM伊文思蓝(EB)诱导的膜超极化,而加入3mM四乙基氯化铵(TEA)的膜电位(MP)变化似乎比用微电极测量的更慢。。通过微电极和DiBAC4(3)的测量,超极化峰值的时间分别为2.3±0.9 s(n = 4)和35.0±2.6 s(n = 12,P <0.01)[1]。

[细胞实验]

在荧光测量之前，将细胞在含有100nM DiBAC4（3）的KRH（Krebs-Ringer-HEPES）缓冲液中在室温下孵育20分钟。染色的细胞无需洗涤即可用于实验。使用505nm双色镜和BA滤光器（> 520nm）收集荧光发射[1]。

[参考文献]

[1]. Yamada A, et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn J Pharmacol. 2001 Jul;86(3):342-50.

[相关活性小分子]

双(1,3-二巴比妥酸)-三次甲基氧烯洛尔物理化学性质

[ 分子式 ]:
C₂₇H₄₀N₄O₆

[ 分子量 ]:
516.63

[ PSA ]:
200.30000

[ LogP ]:
2.90160

[ 储存条件 ]:
2~8°C

双(1,3-二巴比妥酸)-三次甲基氧烯洛尔MSDS

详细MSDS(安全技术说明书)

双(1,3-二巴比妥酸)-三次甲基氧烯洛尔安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xn

[ 危险品运输编码 ]:
NONH for all modes of transport

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