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果胶酶

果胶酶用途

果胶酶是一种水解果胶物质的混合酶,主要存在于微生物和高等植物中。果胶酶参与细胞壁代谢以及细胞生长、衰老、果实成熟、发病机制和脱落过程[1]。

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果胶酶名称

[ CAS 号 ]:
9032-75-1

[ 中文名 ]:
果胶酶

[ 英文名 ]:
Pectinase

[中文别名 ]:

纤维素酶

[英文别名 ]:

MFCD00131809
Polygalacturonase

果胶酶生物活性

[ 描述 ]:

[ 相关类别 ]:

研究领域 >> 其他

信号通路 >> 其他 >> 其他

果胶酶物理化学性质

[ 密度 ]:
1.5±0.1 g/cm3

[ 沸点 ]:
415.5±38.0 °C at 760 mmHg

[ 分子式 ]:
C₁₈H₃₇N(CH₃)₂

[ 分子量 ]:
150.130

[ 闪点 ]:
219.2±23.3 °C

[ 精确质量 ]:
150.052826

[ LogP ]:
-2.39

[ 外观性状 ]:
powder | slightly beige

[ 蒸汽压 ]:
0.0±2.2 mmHg at 25°C

[ 折射率 ]:
1.544

[ 储存条件 ]:
2-8°C

[ 水溶解性 ]:
Soluble in water and practically insoluble in ethanol and ether.

果胶酶安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 安全声明 (欧洲) ]:
24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

果胶酶制备

1.一般用霉菌在含有豆粕、苹果渣、蔗糖等的固体培养基中培养，深层保温通风发酵，然后用水浸提，再用有机溶剂使之沉淀，再分离、干燥、粉碎而得。

黑曲霉由母株诱变所得的突变株，保存于土豆葡萄糖斜面培养基上。培养基在pH=4.7～4.8和28～30℃下通风搅拌培养36～44h，菌体镜检粗状呈网络状，即可转入发酵罐。发酵罐装培养基，杀菌后，冷却到28～30℃，接入种子，在0.08～0.12MPa压力下,通风、搅拌发酵55h。将发酵液压滤，超滤浓缩，加入酒精沉淀，干燥，粉碎可得果胶酶。

以黑曲霉变异菌株经发酵制得。

2.溶组织梭杆菌ATCC21000制取法

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果胶酶文献

A Pectate Lyase-Coding Gene Abundantly Expressed during Early Stages of Infection Is Required for Full Virulence in Alternaria brassicicola.

PLoS ONE 10 , e0127140, (2015)

Alternaria brassicicola causes black spot disease of Brassica species. The functional importance of pectin digestion enzymes and unidentified phytotoxins in fungal pathogenesis has been suspected but ...

Low concentrations of the toxin ophiobolin A lead to an arrest of the cell cycle and alter the intracellular partitioning of glutathione between the nuclei and cytoplasm.

J. Exp. Bot. 66 , 2991-3000, (2015)

Ophiobolin A, a tetracyclic sesterpenoid produced by phytopathogenic fungi, is responsible for catastrophic losses in crop yield but its mechanism of action is not understood. The effects of ophioboli...

Polysaccharide-free nucleic acids and proteins of Abelmoschus esculentus for versatile molecular studies.

Mol. Biol. (Mosk.) 46(4) , 598-604, (2012)

Abelmoschus esculentus (okra) is one of the polysaccharide rich crop plants. The polysaccharides interfere with nucleic acids and protein isolation thereby affecting the downstream molecular analysis....

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果胶酶