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派洛宁Y

派洛宁Y用途

Pyronin Y是对RNA特异性的插层阳离子染料。

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派洛宁Y名称

[ CAS 号 ]:
92-32-0

[ 中文名 ]:
焦宁Y

[ 英文名 ]:
Pyronin Y

[中文别名 ]:

[英文别名 ]:

Pyronine Y
Tetramethyl pyronin
EINECS 202-147-8
Pyronine
Pyroniny(G)
Methyl pyronin
Pyronin G
Pyronine G chloride
Pyronine ZH
MFCD00011725

派洛宁Y生物活性

[ 描述 ]:

Pyronin Y是对RNA特异性的插层阳离子染料。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

Pyronin Y与双链核酸,特别是RNA形成荧光复合物,能够在流式细胞仪中对细胞RNA进行半定量分析,估算福尔马林固定EL4白血病肿瘤细胞中每个细胞的RNA含量,酶分散R3327-G大鼠前列腺腺癌用刀豆蛋白A刺激小鼠脾细胞,用植物血凝素刺激人外周血淋巴细胞[1]。描述了基于pyronin Y的荧光染色方法。该技术已被用于染色人网织红细胞中的RNA,用于随后的流动分析和分选[2]。在活细胞中,这种染料也在线粒体中积累。浓度为1.7至3.3μM时,pyronin Y几乎仅位于培养细胞的线粒体中,与另一种线粒体相似！探针,罗丹明123.在该浓度下,PY无毒但抑制细胞生长,阻滞细胞[3]。长期以来,Pyronin Y与其他染料如Methyl Green一起用作石蜡组织切片中核酸的差异染色。在用甲基绿- pyronin Y染色的切片中,红细胞,血管弹性纤维,胰腺腺泡细胞的酶原颗粒,肝细胞和肾小管细胞的表面膜显示出惊人强烈的绿色和/或红色荧光,而细胞核出现非荧光[4]。

[细胞实验]

将细胞重新悬浮并在sodiumacetate缓冲液（1.0M，pH4.7）中的1.0mL 0.01％pyronin Y中染色30分钟。为此目的，将1.0g的pyronin Y溶解在100mL蒸馏水中，并通过氯仿萃取（三份100mL）纯化该溶液。将纯化的pyronin Y溶液用1.0M sodiumacetate缓冲液（pH4.7）稀释至合适的浓度，并在使用前通过纸过滤器过滤。通过荧光显微镜（激发滤光片SP 560和LP 515，580nm处的彩色分束器，屏障滤光片LP 580）或通过流式细胞术[2]研究染色细胞样品。

[参考文献]

[1]. Pollack A, et al. Flow cytometric analysis of RNA content in different cell populations using pyronin Yand methyl green. Cytometry. 1982 Jul;3(1):28-35.

[2]. Tanke HJ, et al. Flow cytometry of human reticulocytes based on RNA fluorescence. Cytometry. 1981 Mar;1(5):313-20.

[3]. Darzynkiewicz Z, et al. Cytostatic and cytotoxic properties of pyronin Y: relation to mitochondrial localization of the dye and its interaction with RNA. Cancer Res. 1986 Nov;46(11):5760-6.

[4]. Li B, et al. Pyronin Y as a fluorescent stain for paraffin sections. Histochem J. 2002 Jun-Jul;34(6-7):299-303.

[相关活性小分子]

派洛宁Y物理化学性质

[ 熔点 ]:
250-260ºC(lit.)

[ 分子式 ]:
C₁₇H₁₉ClN₂O

[ 分子量 ]:
302.799

[ 精确质量 ]:
302.118591

[ PSA ]:
19.39000

[ 外观性状 ]:
暗绿色至棕色晶体

[ 储存条件 ]:
Store at RT.

[ 水溶解性 ]:
Soluble in water; sparingly soluble in ethanol, ethylene glycol, methyl cellosolve | Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL).

派洛宁YMSDS

详细MSDS(安全技术说明书)

派洛宁Y毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: BQ1450000

CHEMICAL NAME :: Ammonium, (6-(dimethylamino)-3H-xanten-3-ylidene)dimethyl-, chloride

CAS REGISTRY NUMBER :: 92-32-0

LAST UPDATED :: 199701

DATA ITEMS CITED :: 9

MOLECULAR FORMULA :: C17-H19-N2-O.Cl

MOLECULAR WEIGHT :: 302.83

WISWESSER LINE NOTATION :: T C666 BO EYJ EUK1&1 MN1&1 &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 179 mg/kg

TOXIC EFFECTS :: Kidney, Ureter, Bladder - changes in tubules (including acute renal failure, acute tubular necrosis)

MUTATION DATA

TEST SYSTEM :: Insect - Drosophila melanogaster

DOSE/DURATION :: 1000 ppm

REFERENCE :: AMNTA4 American Naturalist. (Univ. of Chicago Press, Journals Div., POB 37005, Chicago, IL 60637) V.1- 1867- Volume(issue)/page/year: 87,295,1953 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X7179 No. of Facilities: 63 (estimated) No. of Industries: 1 No. of Occupations: 3 No. of Employees: 1056 (estimated) No. of Female Employees: 810 (estimated)

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派洛宁Y安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xn: Harmful;

[ 风险声明 (欧洲) ]:
R68

[ 安全声明 (欧洲) ]:
22-24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ RTECS号 ]:
BQ1450000

[ 海关编码 ]:
2921199090

派洛宁Y合成路线

详细合成路线

派洛宁Y制备

由N,N-二甲基间氨基苯酚为原料制取。

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派洛宁Y海关

[ 海关编码 ]: 2921199090

[ 中文概述 ]:
2921199090 其他无环单胺及其衍生物及其盐。监管条件:无。增值税率:17.0%。退税率:9.0%。最惠国关税:6.5%。普通关税:30.0%

[ 申报要素 ]: 品名, 成分含量, 用途

[ Summary ]:
2921199090 other acyclic monoamines and their derivatives; salts thereof VAT:17.0% Tax rebate rate:9.0% Supervision conditions:none MFN tariff:6.5% General tariff:30.0%

派洛宁Y文献

Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analysed by flow cytometry.

J. Med. Microbiol. 63(Pt 12) , 1663-9, (2014)

The major risk factor for Clostridium difficile infection (CDI) is the use of antibiotics owing to the disruption of the equilibrium of the host gut microbiota. To preserve the beneficial resident pro...

Evaluation of posterolateral lumbar fusion in sheep using mineral scaffolds seeded with cultured bone marrow cells.

Int. J. Mol. Sci. 15(12) , 23359-76, (2014)

The objective of this study is to investigate the efficacy of hybrid constructs in comparison to bone grafts (autograft and allograft) for posterolateral lumbar fusion (PLF) in sheep, instrumented wit...

The orphan nuclear receptor NR4A1 specifies a distinct subpopulation of quiescent myeloid-biased long-term HSCs.

Stem Cells 33(1) , 278-88, (2014)

Hematopoiesis is maintained throughout life by self-renewing hematopoietic stem cells (HSCs) that differentiate to produce both myeloid and lymphoid cells. The NR4A family of orphan nuclear receptors,...

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派洛宁Y

派洛宁Y用途

派洛宁Y名称

派洛宁Y生物活性

派洛宁Y物理化学性质

派洛宁YMSDS

详细MSDS(安全技术说明书)

派洛宁Y毒性和生态

CHEMICAL IDENTIFICATION

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

派洛宁Y安全信息

派洛宁Y合成路线

详细合成路线

派洛宁Y制备

派洛宁Y海关

派洛宁Y文献

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