4-二甲氨基偶氮苯-4'-异硫氰酸酯
4-二甲氨基偶氮苯-4'-异硫氰酸酯结构式
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常用名 | 4-二甲氨基偶氮苯-4'-异硫氰酸酯 | 英文名 | 4-(4-Isothiocyanatophenylazo)-N,N-dimethylaniline |
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| CAS号 | 7612-98-8 | 分子量 | 282.363 | |
| 密度 | 1.1±0.1 g/cm3 | 沸点 | 466.2±30.0 °C at 760 mmHg | |
| 分子式 | C15H14N4S | 熔点 | 167-171 °C(lit.) | |
| MSDS | 中文版 美版 | 闪点 | 235.7±24.6 °C | |
| 符号 |
GHS08 |
信号词 | Danger |
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A new isotype sequence (V kappa 27) of the variable region of kappa-light chains from a mouse hybridoma-derived anti-(streptococcal group A polysaccharide) antibody containing an additional cysteine residue. Application of the dimethylaminoazobenzene isothiocyanate technique for the isolation of peptides.
Biochem. J. 211(1) , 173-80, (1983) The first complete sequence of the variable region of a kappa-light chain (V kappa) from a mouse anti-(streptococcal group A polysaccharide) antibody (immunoglobulin 7S34.1) is reported. Immunoglobulin 7S34.1 was isolated from the ascitic fluid of hybridoma 7... |
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Peptide maps at picomolar levels obtained by reversed-phase high-performance liquid chromatography and pre-column derivatization with phenyl isothiocyanate. Microsequencing of Phenylthiocarbamyl Peptides.
J. Chromatogr. A. 548(1-2) , 303-10, (1991) A new reversed-phase high-performance liquid chromatography approach to the production of analytical peptide maps by pre-column derivatization using phenylisothiocyanate is described. Tryptic peptide digests were derivatized with phenyl isothiocyanate to form... |
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Evidence for the formation of a novel glutathione conjugate in the metabolism of an aromatic amine derivative.
Drug Metab. Dispos. 12(4) , 523-4, (1984)
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Novel histamine measurement by HPLC analysis used to assay histidine decarboxylase inhibitory activity of shoyuflavones from soy sauce.
Biosci. Biotechnol. Biochem. 62(8) , 1488-91, (1998) An easy and highly sensitive method for measuring histamine by HPLC analysis coupled with precolumn derivatization was established. The amino group of histamine was completely colorimetrically labelled with 4-N,N-dimethylamino-azobenzene-4'-isothiocyanate (DA... |
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Improvements in the application of the 4,4-N,N-dimethylaminoazobenzene-4'-isothiocyanate micromethod to the sequence analysis of proteins.
Anal. Biochem. 180(2) , 374-9, (1989) Different experimental conditions have been tested to improve the sequence determination of peptides and proteins by the DABITC (4,4-N,N-dimethylaminoazobenzene-4'-isothiocyanate) method and to facilitate automation in the analysis of the released 4,4-N,N-dim... |
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Analysis of 4-N,N-dimethylaminoazobenzene 4'-thiohydantoin amino acids at sub-picomole levels by high-performance liquid chromatography: simultaneous manual sequencing of picomole quantities of several polypeptides.
Anal. Biochem. 177(1) , 46-9, (1989) A single-column high-performance liquid chromatographic separation of 4-N,N-dimethylaminoazobenzene 4'-thiohydantoin amino acid derivatives, generated during polypeptide sequence analysis by the 4-N,N-dimethylaminoazobenzene 4'-isothiocyanate/phenylisothiocya... |
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Identification of a reactive lysyl residue (Lys103) of recombinant human interleukin-1 beta. Mechanism of its reactivity and implication of its functional role in receptor binding.
Biochemistry 31(11) , 2874-8, (1992) Recombinant human interleukin-1 beta (h-IL-1 beta) was chemically modified with 4-(N,N-dimethylamino)-4'-isothiocyanatoazobenzene-2'-sulfonic acid (S-DABITC), a water-soluble color reagent specific for lysine labeling. Modified h-IL-1 beta was digested by lys... |
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Isolation and HPLC of N-epsilon-lithocholyl lysine as its fluorescamine and dimethylaminoazobenzene isothiocyanate derivatives.
Steroids 59(3) , 212-6, (1994) N-epsilon-lithocholyl lysine (NELL) is a component of tissue-bound lithocholic acid (TBL). The isolation of NELL from native protein sources was simulated by hydrolysis of lithocholyl-bovine serum albumin (BSA) (synthesized by coupling lithocholyl-N-hydroxysu... |
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Microisolation of DABITC-derivatized protein by gel electrophoresis: application to the purification of antibody heavy and light chains.
Anal. Biochem. 136(2) , 465-9, (1984) Proteins and polypeptides are derivatized with dimethylaminoazobenzene isothiocyanate (DABITC) before their separation on sodium dodecyl sulfate-polyacrylamide gels. DABITC-derivatized proteins are detected visually in the picomole range without further stain... |
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Solid-phase protein and peptide sequencing using either 4-N,N-dimethylaminoazobenzene 4'-isothiocyanate or phenylisothiocyanate.
Arch. Biochem. Biophys. 235(1) , 48-62, (1984) Automated solid-phase sequencing using 4-N,N-dimethylaminoazobenzene 4'-isothiocyanate (DABITC) double coupling or regular phenylisothiocyanate (PITC) degradation procedures have been investigated. Employing sensitive high-performance liquid chromatography fo... |