1449236-96-7

1449236-96-7 structure

Name: NMS-859
Chemical Name: NMS-859
CAS Number: 1449236-96-7
Molecular Formula: C₁₅H₁₂ClN₃O₃S
Molecular Weight: 349.789
Catalog: Signaling Pathways Cell Cycle/DNA Damage p97
Create Date: 2018-06-19 02:10:56
Modify Date: 2025-08-20 12:28:15
NMS-859 is a potent, covalent VCP (p97) inhibitor, with IC50s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively.

Name	NMS-859
Synonyms	3-[(1,1-Dioxido-1,2-benzothiazol-3-yl)amino]phenyl chloroacetate MFCD05030984 Acetic acid, 2-chloro-, 3-[(1,1-dioxido-1,2-benzisothiazol-3-yl)amino]phenyl ester

Description	NMS-859 is a potent, covalent VCP (p97) inhibitor, with IC50s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively.
Related Catalog	Signaling Pathways >> Cell Cycle/DNA Damage >> p97 Research Areas >> Cancer
Target	IC50: 360 nM (Cellular p97, 1 mM ATP), 370 nM (Cellular p97, 60 μM ATP)[1]
In Vitro	NMS-859 is a potent VCP inhibitor, with IC50s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively. NMS-859 shows very weak inhibitory activity against VCPC522T. NMS-859 also suppresses the proliferation of cells, with IC50s of 3.5 μM and 3.0 μM in HCT116 and HeLa cell lines, respectively[1].
Cell Assay	Cells are seeded at 1,600 cells per well in 384-well white clear-bottom plates. Twenty-four hours after seeding, cells are treated with NMS-859 (eight dilution points, in duplicate) and incubated for an additional 72 h at 37°C under a 5% CO2 atmosphere. Cells are then lysed, and the ATP content in each well is determined using a thermostable firefly luciferase-based assay as a measure of cell viability. IC50 values are calculated using the percentage of growth of treated cells versus the untreated control[1].
References	[1]. Magnaghi P, et al. Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death. Nat Chem Biol. 2013 Sep;9(9):548-56.