Top Suppliers:I want be here


  • DC Chemicals Limited
  • China
  • Product Name: RN-18
  • Price: $550.0/100mg $900.0/250mg $1700.0/1g
  • Purity: 98.0%
  • Stocking Period: 3 Day
  • Contact: Tony Cao

431980-38-0

431980-38-0 structure
431980-38-0 structure
  • Name: RN-18
  • Chemical Name: N-(2-methoxyphenyl)-2-(4-nitrophenyl)sulfanylbenzamide
  • CAS Number: 431980-38-0
  • Molecular Formula: C20H16N2O4S
  • Molecular Weight: 380.41700
  • Catalog: Signaling Pathways Anti-infection HIV
  • Create Date: 2017-10-17 10:09:38
  • Modify Date: 2024-01-11 21:12:50
  • RN-18 is a HIV-1 viral infectivity factor (HIV-1 Vif) inhibitor with an IC50 of 6 μM in nonpermissive H9 cells.

Name N-(2-methoxyphenyl)-2-(4-nitrophenyl)sulfanylbenzamide
Synonyms rn-18
Description RN-18 is a HIV-1 viral infectivity factor (HIV-1 Vif) inhibitor with an IC50 of 6 μM in nonpermissive H9 cells.
Related Catalog
Target

IC50: 6 μM (nonpermissive H9 cell)[1]

In Vitro RN-18 and RN-19 exhibits potent antiviral activity in the nonpermissive H9 and CEM cells but not in MT4 or CEM-SS cells, confirming that the antiviral activity was Vif specific. RN-18 shows the greater potency (IC50=4.5 μM in CEM cells) and specificity (IC50>100 μM in MT4 cells) among the two compounds[1]. In the presence of the inhibitor, RN-18, reverse transcriptase activity in the nonpermissive H9 and CEM cells decreases substantially and in a dose-dependent manner. RN-18 also exhibits antiviral activity in CEM-SS modified to stably express A3G but does not exhibit antiviral activity in the parental CEM-SS cell line. RN-18 antagonizes Vif function and inhibits HIV-1 replication only in the presence of A3G. RN-18 increases cellular A3G levels in a Vif-dependent manner and increases A3G incorporation into virions without inhibiting general proteasome-mediated protein degradation. RN-18 enhances Vif degradation only in the presence of A3G, reduces viral infectivity by increasing A3G incorporation into virions and enhances cytidine deamination of the viral genome[2].
Cell Assay H9 or MT4 cells are treated overnight with 0, 1, 5, 10, 25 or 50 μM RN-18 (all at 0.1% DMSO) and infected with HIV-1. All cells are maintained in the presence of DMSO or RN-18 for 14 d, and viral replication is monitored every 2 d by measuring reverse transcriptase activity in culture supernatants. The average % relative infectivity at day 7 is determined from 3 separate reverse transcriptase assays. Grafit software is used to fit curves and to determine IC50[2].
References

[1]. Mohammed I, et al. SAR and Lead Optimization of an HIV-1 Vif-APOBEC3G Axis Inhibitor. ACS Med Chem Lett. 2012 Jun 14;3(6):465-469.

[2]. Nathans R, et al. Small-molecule inhibition of HIV-1 Vif. Nat Biotechnol. 2008 Oct;26(10):1187-92.

Molecular Formula C20H16N2O4S
Molecular Weight 380.41700
Exact Mass 380.08300
PSA 112.94000
LogP 5.91410
Storage condition 2-8℃