122965-43-9

122965-43-9 structure

Name: METHYLENE BLUE
Chemical Name: methylene blue
CAS Number: 122965-43-9
Molecular Formula: C₁₆H₂₀ClN₃OS
Molecular Weight: 337.86800
Catalog: Biochemical Biochemical reagent Acid-base indicator
Create Date: 2018-02-03 08:00:00
Modify Date: 2025-08-20 14:30:11
Methylene blue (Basic Blue 9) hydrate is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue hydrate through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue hydrate is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue hydrate is a Tau aggregation inhibitor. Methylene blue hydrate reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation[1][2][3].

Name	methylene blue
Synonyms	Methylene Blue hydrate,high purity biological stain MFCD00150006 Methylene Blue,high purity Methylene Blue MethyleneblueHydrat Methylene Blue hydrate Methylthioninium chloride EINECS 200-515-2 Methylene Blue hydrate,Methylthionini chloridum

Description	Methylene blue (Basic Blue 9) hydrate is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue hydrate through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue hydrate is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue hydrate is a Tau aggregation inhibitor. Methylene blue hydrate reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation[1][2][3].
Related Catalog	Research Areas >> Cancer Research Areas >> Infection Signaling Pathways >> Cell Cycle/DNA Damage >> Microtubule/Tubulin Signaling Pathways >> Immunology/Inflammation >> NO Synthase Signaling Pathways >> Neuronal Signaling >> Monoamine Oxidase Research Areas >> Inflammation/Immunology Signaling Pathways >> Cytoskeleton >> Microtubule/Tubulin Signaling Pathways >> GPCR/G Protein >> Guanylate Cyclase
In Vitro	Methylene blue (Basic Blue 9) hydrate (4.5 μM; BV2 microglia) alters the immune profile of LPS-activated BV2 microglia and decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA[3].
In Vivo	Methylene blue (Basic Blue 9) hydrate (50 and 100 mg/kg; i.p.; once, for 25 min; male NMRI mice) reduces absent prepulse inhibition[1]. Methylene blue (Basic Blue 9) hydrate (20 and 40 mg/kg; p.o.; daily, for 6 months; CaMKIIα-tTA transactivator mice) preserves cognition in mice expressing full-length pro-aggregant human Tau[2]. Methylene blue (Basic Blue 9) hydrate (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces TBI-induced edema and neuroinflammation and reduces acute depression-like behavior[3]. Methylene blue (Basic Blue 9) hydrate (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces the percentage of inflammatory factor[3]. Animal Model: Male NMRI mice[1] Dosage: 50 and 100 mg/kg Administration: Intraperitoneal injection; once, for 25 minutes Result: Reduced the prepulse inhibition and reduced the increase in locomotor activity caused by phencyclidine (PCP). Animal Model: CaMKIIα-tTA transactivator mice[2] Dosage: 20 and 40 mg/kg Administration: Oral administration; daily, for 6 months Result: Inhibited Tau aggregation in CaMKIIα-tTA transactivator mice. Animal Model: TBI-treated male BALB/c mice[3] Dosage: 2 mg/kg Administration: Intravenous injection; once, for 1 day Result: Decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA. Animal Model: TBI-treated male BALB/c mice[3] Dosage: 2 mg/kg Administration: Intravenous injection; once, for 1 day Result: Reduced the percentage of myeloid (CD11b+/GR1+) cells, reduced IL-1β and enhanced IL-10 expression in microglia.
References	[1]. Klamer D, et, al. Phencyclidine-induced behaviour in mice prevented by methylene blue. Basic Clin Pharmacol Toxicol. 2004 Feb;94(2):65-72. [2]. Hochgräfe K, et, al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015 May 10;3:25. [3]. Fenn AM, et, al. Methylene blue attenuates traumatic brain injury-associated neuroinflammation and acute depressive-like behavior in mice. J Neurotrauma. 2015 Jan 15;32(2):127-38.

Density	0.6600g/ml
Melting Point	190ºC
Molecular Formula	C₁₆H₂₀ClN₃OS
Molecular Weight	337.86800
Exact Mass	337.10200
PSA	56.61000
Appearance	Fine Crystalline Powder \| Green
Storage condition	room temp
Water Solubility	H2O: soluble4mg/4ml \| Soluble in water, alcohol, acetic acid, glycerol and chloroform. Insoluble in ether.

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H302
Precautionary Statements	P301 + P312 + P330
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xn:Harmful
Risk Phrases	R22
Safety Phrases	26-39-61
RIDADR	UN 2811 6.1/PG 3
WGK Germany	3
RTECS	SO5600000

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