| In Vitro |
Tubulin polymerization-IN-14 (Compound 20a) binds to the colchicine binding site on tubulin[1]. Tubulin polymerization-IN-14 (0-1 μM; 72 h) inhibits cancer cell growth[1]. Tubulin polymerization-IN-14 (5-20 nM; 48 h) arrests K562 cell cycle at G2/M phase, significantly induces cell apoptosis in K562 cells in a concentration-dependent manner, and induces mitochondrial membrane potential (MMP) collapse and mitochondrial dysfunction in K562 cells[1]. Tubulin polymerization-IN-14 (5-20 nM; 24 h) significantly decreases wound closure and capillary-like tubules formation in a concentration-dependent manner in HUVECs[1]. Cell Proliferation Assay[1] Cell Line: K562 cells Concentration: 0-1 μM Incubation Time: 72 h Result: Showed anti-proliferative activity with an IC50 of 0.01 ± 0.001 μM against K562 cells. Cell Cytotoxicity Assay[1] Cell Line: HepG2, HCT-8, MDA-MB-231 and HFL-1 cells Concentration: 0-1 μM Incubation Time: 72 h Result: Showed cytotoxic activities with IC50s of 0.019 ± 0.002, 0.021 ± 0.003, 0.02 ± 0.001 and 0.118 ± 0.007 μM against HepG2, HCT-8, MDA-MB-231 and HFL-1 cells, respectively. Cell Cycle Analysis[1] Cell Line: K562 cells Concentration: 5 nM, 10 nM and 20 nM Incubation Time: 48 h Result: 9.40%, 11.54% and 15.28% of cells were arrested at G2/M phase at 5, 10 and 20 nM, respectively. Apoptosis Analysis[1] Cell Line: K562 cells Concentration: 5 nM, 10 nM and 20 nM Incubation Time: 48 h Result: Compared to the percentage of apoptosis cells in control group (3.25%), the total percentage of the early (Annexin-Vþ/PI) and late (Annexin-Vþ/PIþ) apoptosis cells were 10.46%, 48.55% and 62.26% after being treated at 5, 10, and 20 nM for 48 h, respectively. Cell Migration Assay [1] Cell Line: HUVECs Concentration: 5 nM, 10 nM and 20 nM Incubation Time: 24 h Result: After exposed to compound at 5, 10, and 20 nM for 24 h, cells migrated into 67.6%, 55.3% and 49.2% of the wound area, respectively.
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