(-)-Demecolcine

Modify Date: 2024-01-04 15:17:56

(-)-Demecolcine structure	Common Name	(-)-Demecolcine
	CAS Number	477-30-5	Molecular Weight	371.427
	Density	1.2±0.1 g/cm3	Boiling Point	625.5±55.0 °C at 760 mmHg
	Molecular Formula	C₂₁H₂₅NO₅	Melting Point	73-75ºC
	MSDS	Chinese USA	Flash Point	332.1±31.5 °C
	Symbol	GHS06	Signal Word	Danger

Use of (-)-Demecolcine

Colcemid (Demecolcine), a derivative of colchicine, is a potent mitotic inhibitor[1][2]. Colcemid binds to the protein tubulin and arrest cells in metaphase for karyotyping assays. Colcemid incuces cell apoptosis and can be used for cancer research[2].

Name	(-)-demecolcine
Synonym	More Synonyms

Description	Colcemid (Demecolcine), a derivative of colchicine, is a potent mitotic inhibitor[1][2]. Colcemid binds to the protein tubulin and arrest cells in metaphase for karyotyping assays. Colcemid incuces cell apoptosis and can be used for cancer research[2].
Related Catalog	Research Areas >> Cancer Signaling Pathways >> Cell Cycle/DNA Damage >> Microtubule/Tubulin Research Areas >> Inflammation/Immunology Signaling Pathways >> Cytoskeleton >> Microtubule/Tubulin
References	[1]. T Tsuchida, et al. Colcemid-induced apoptosis of cultured human glioma: electron microscopic and confocal laser microscopic observation of cells sorted in different phases of cell cycle. Cytometry. 1998 Apr 1;31(4):295-9. [2]. Ashley M Rozario, et al. Ultra-Low Colcemid Doses Induce Microtubule Dysfunction as Revealed by Super-Resolution Microscopy. Bioexiv.

Description

Related Catalog

Research Areas >> Cancer

Signaling Pathways >> Cell Cycle/DNA Damage >> Microtubule/Tubulin

Research Areas >> Inflammation/Immunology

Signaling Pathways >> Cytoskeleton >> Microtubule/Tubulin

References

[1]. T Tsuchida, et al. Colcemid-induced apoptosis of cultured human glioma: electron microscopic and confocal laser microscopic observation of cells sorted in different phases of cell cycle. Cytometry. 1998 Apr 1;31(4):295-9.

[2]. Ashley M Rozario, et al. Ultra-Low Colcemid Doses Induce Microtubule Dysfunction as Revealed by Super-Resolution Microscopy. Bioexiv.

Density	1.2±0.1 g/cm3
Boiling Point	625.5±55.0 °C at 760 mmHg
Melting Point	73-75ºC
Molecular Formula	C₂₁H₂₅NO₅
Molecular Weight	371.427
Flash Point	332.1±31.5 °C
Exact Mass	371.173279
PSA	66.02000
LogP	1.52
Vapour Pressure	0.0±1.8 mmHg at 25°C
Index of Refraction	1.582

(-)-Demecolcine MSDS(Chinese)

CHEMICAL IDENTIFICATION

RTECS NUMBER :: GH0800000

CHEMICAL NAME :: Colchicine, N-deacetyl-N-methyl-

CAS REGISTRY NUMBER :: 477-30-5

BEILSTEIN REFERENCE NO. :: 2822892

LAST UPDATED :: 199801

DATA ITEMS CITED :: 49

MOLECULAR FORMULA :: C21-H25-N-O5

MOLECULAR WEIGHT :: 371.47

WISWESSER LINE NOTATION :: L B677 MV&T&J CO1 DO1 EO1 JM1 NO1

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Human

DOSE/DURATION :: 200 ug/kg

TOXIC EFFECTS :: Skin and Appendages - hair

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 1700 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Parenteral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 1700 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 25530 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 35 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 56 mg/kg

TOXIC EFFECTS :: Behavioral - somnolence (general depressed activity) Behavioral - muscle weakness Gastrointestinal - hypermotility, diarrhea

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intramuscular

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 87 mg/kg

TOXIC EFFECTS :: Nutritional and Gross Metabolic - weight loss or decreased weight gain

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Subcutaneous

DOSE :: 3 mg/kg

SEX/DURATION :: female 7-9 day(s) after conception

TOXIC EFFECTS :: Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants) Reproductive - Effects on Embryo or Fetus - fetal death

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 1800 ug/kg

SEX/DURATION :: female 8 day(s) after conception

TOXIC EFFECTS :: Reproductive - Specific Developmental Abnormalities - Central Nervous System Reproductive - Specific Developmental Abnormalities - eye/ear Reproductive - Specific Developmental Abnormalities - body wall

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 1800 ug/kg

SEX/DURATION :: female 8 day(s) after conception

TOXIC EFFECTS :: Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants)

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Subcutaneous

DOSE :: 500 ug/kg

SEX/DURATION :: female 12 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Embryo or Fetus - fetal death

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Unreported

DOSE :: 2500 ug/kg

SEX/DURATION :: female 2-26 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus)

TYPE OF TEST :: Sex chromosome loss and nondisjunction

TYPE OF TEST :: Sperm Morphology

TYPE OF TEST :: Micronucleus test

TYPE OF TEST :: Cytogenetic analysis

TYPE OF TEST :: Sex chromosome loss and nondisjunction

MUTATION DATA

TYPE OF TEST :: Sex chromosome loss and nondisjunction

TEST SYSTEM :: Mammal - species unspecified

DOSE/DURATION :: 2 ug/L

REFERENCE :: MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 201,423,1988 *** REVIEWS *** TOXICOLOGY REVIEW JAMAAP JAMA, Journal of the American Medical Association. (AMA, 535 N. Dearborn St., Chicago, IL 60610) V.1- 1883- Volume(issue)/page/year: 172,1765,1960 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X3189 No. of Facilities: 105 (estimated) No. of Industries: 2 No. of Occupations: 2 No. of Employees: 412 (estimated) No. of Female Employees: 287 (estimated)

Symbol	GHS06
Signal Word	Danger
Hazard Statements	H300
Precautionary Statements	P264-P301 + P310
Personal Protective Equipment	Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)
Hazard Codes	T: Toxic;
Risk Phrases	R25
Safety Phrases	22-24/25-45-36/37/39
RIDADR	UN 2811
WGK Germany	3
RTECS	GH0800000
Packaging Group	II
Hazard Class	6.1(a)

Precursor 9
CAS#:14686-61-4 Formamide,N-met... CAS#:64-86-8 Colchicine CAS#:477-27-0 Colchiceine CAS#:3482-37-9 Trimethylcolchi...
CAS#:3476-50-4 Trimethylcolchi... CAS#:74-88-4 methyl iodide CAS#:186581-53-3 diazomethane CAS#:518-11-6 COLCHICEINE, N-...
CAS#:7359-91-3 (7S)-2-hydroxy-...
DownStream 7
CAS#:518-11-6 COLCHICEINE, N-... CAS#:14686-61-4 Formamide,N-met... CAS#:16892-03-8 Benzo[a]heptale... CAS#:7336-40-5 N-Methylcolchicine
CAS#:7336-44-9 Demecolcine, N-... CAS#:76129-11-8 Benzo[a]heptale... CAS#:86436-33-1 N-Hydroxyacetyl...

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Microwave assisted extraction (MAE) followed by microplate solid phase extraction (MPSPE) coupled with ultra high performance liquid chromatography (UHPLC) for the semi-quantitative determination of c...

High-affinity accumulation of a maytansinoid in cells via weak tubulin interaction.

PLoS ONE 10(2) , e0117523, (2015)

The microtubule-targeting maytansinoids accumulate in cells and induce mitotic arrest at 250- to 1000-fold lower concentrations than those required for their association with tubulin or microtubules. ...

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Benzo[a]heptalen-9(5H)-one, 6,7-dihydro-1,2,3,10-tetramethoxy-7-(methylamino)-, (7S)-

Benzo[a]heptalen-9(5H)-one, 6,7-dihydro-1,2,3,10-tetramethoxy-7-(methylamino)-, (S)-

Demecolcine

Benzo(a)heptalen-9(5H)-one, 6,7-dihydro-1,2,3,10-tetramethoxy-7-(methylamino)-, (S)-

(7S)-1,2,3,10-Tetramethoxy-7-(methylamino)-6,7-dihydrobenzo[a]heptalen-9(5H)-one

demecolcinum

Colcemid

EINECS 207-514-6

demecolcina

N-Deacetyl-N-methylcolchicine

MFCD00075459

Shanghai Jizhi Biochemical Technology Co., Ltd
China
Product Name: (-)-Demecolcine
Price: ￥948.0/5mg ￥3298.0/25mg
Purity: 98.0%
Stocking Period: Inquiry
Contact: Liu jia

DC Chemicals Limited
China
Product Name: Demecolcine
Price: $280.0/20mg
Purity: 98.0%
Stocking Period: 3 Day
Contact: Tony Cao

Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: Demecolcine
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
Stocking Period: Inquiry
Contact: Ms Wang

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(-)-Demecolcine

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