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赫斯特荧光燃料,33258

赫斯特荧光燃料,33258用途

Hoechst 33258 trihydrochloride 是一种荧光染料,用于 DNA 染色。

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赫斯特荧光燃料,33258名称

[ CAS 号 ]:
23491-45-4

[ 中文名 ]:
赫斯特荧光染料 33258

[ 英文名 ]:
Hoechst 33258 (trihydrochloride)

[中文别名 ]:

[英文别名 ]:

BBIH
BISBENZIMIDE H 33258
Hoechst33258
Hoechst Stain
BISBENZIMIDE
Bisbenzimide H 33258 trihydrochloride
Bisbenzimide H 33258 Hydrate
MFCD00012679
Hoechst 33258
Pibenzimol HCl

赫斯特荧光燃料,33258生物活性

[ 描述 ]:

Hoechst 33258 trihydrochloride 是一种荧光染料,用于 DNA 染色。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 癌症

[ 靶点 ]

IC50: 51.31±4.56 μM (HeLa cell), 32.43±3.27 μM (HL60 cell), 15.42±2.16 μM (U937 cell)[1]

[体外研究]

Hoechst 33258是一种具有头对尾双苯并咪唑结构的荧光化合物,最初被发现对L1210鼠白血病具有细胞毒性。评估Hoechst 33258对人肿瘤细胞系的细胞毒性,所述人肿瘤细胞系是宫颈癌细胞系(HeLa),人早幼粒细胞白血病细胞(HL60)和U937细胞系。对于Hoechst 33258,在HeLa,HL60和U937的情况下测定的IC50分别为51.31±4.56,32.43±3.27和15.42±2.16μM[1]。 Hoechst 33258的细胞毒性在一组七个不同组织学起源的肿瘤细胞系和Madine-Darby犬肾(MDCK)正常细胞上进行研究。暴露于Hoechst 33258的除MCF-7外的所有细胞系显示GI50为84×10-6至191.5×10-6mol/dm3。在相同的实验条件下,用作粘合剂参比化合物的Hoechst 33258在S期和G0/G1期停止细胞周期[2]。

[细胞实验]

Hoechst 33258是高纯水中的储备溶液。在测试之前制备浓度范围为10-3-10-6mol / dm3的工作溶液。通过MTT测定确定Hoechst 33258对测试细胞系的细胞毒性作用。将细胞以2×10 4个细胞/ mL的浓度接种在96微孔平底板中，并在CO 2培养箱中放置过夜，使它们附着在板表面上。将生长培养基替换为补充化合物或对照培养基并孵育72小时。将含有5mg / mL MTT的新鲜培养基加入细胞中并在37℃下孵育4小时。除去培养基后，将在活细胞内形成的水不溶性MTT-甲crystals晶体溶解在DMSO中，并在Elisa Microplate Reader上测量与活细胞数成比例的570nm处的吸光度。所有实验一式三份进行至少三次。计算GI50值，定义为导致细胞生长抑制50％的化合物浓度（μM），并用作比较化合物之间细胞毒性的参数[2]。

[参考文献]

[1]. Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through inductionof apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.

[2]. Stolić I, et al. Synthesis, DNA/RNA affinity and antitumour activity of new aromatic diamidines linked by 3,4-ethylenedioxythiophene. Eur J Med Chem. 2011 Feb;46(2):743-55.

[相关活性小分子]

赫斯特荧光燃料,33258物理化学性质

[ 沸点 ]:
643.1ºC at 760mmHg

[ 熔点 ]:
314 °C

[ 分子式 ]:
C₂₅H₂₇Cl₃N₆O

[ 分子量 ]:
533.880

[ 闪点 ]:
342.7ºC

[ 精确质量 ]:
532.131165

[ PSA ]:
84.07000

[ LogP ]:
6.63950

[ 外观性状 ]:
黄色-绿色粉末

[ 储存条件 ]:
2-8°C

[ 水溶解性 ]:
SOLUBLE

赫斯特荧光燃料,33258MSDS

详细MSDS(安全技术说明书)

赫斯特荧光燃料,33258毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SM1140500

CHEMICAL NAME :: Phenol, p-(5-(5-(4-methyl-1-piperazinyl)-2-benzimidazolyl)-2- benzimidazolyl)-, trihydrochloride

CAS REGISTRY NUMBER :: 23491-45-4

LAST UPDATED :: 199709

DATA ITEMS CITED :: 14

MOLECULAR FORMULA :: C25-H24-N6-O.3Cl-H

MOLECULAR WEIGHT :: 533.93

WISWESSER LINE NOTATION :: T56 BM DNJ CR DQ& G- CT56 BM DNJ G- AT6N DNTJ D1 &GH 3

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 32200 ug/kg

TOXIC EFFECTS :: Behavioral - somnolence (general depressed activity) Lungs, Thorax, or Respiration - dyspnea Skin and Appendages - hair

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 36900 ug/kg

TOXIC EFFECTS :: Behavioral - somnolence (general depressed activity) Lungs, Thorax, or Respiration - dyspnea Skin and Appendages - hair

MUTATION DATA

TYPE OF TEST :: DNA damage

TEST SYSTEM :: Mammal - species unspecified Lymphocyte

DOSE/DURATION :: 1600 nmol/L

REFERENCE :: CHROAU Chromosoma. (Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ 07094) V.1- 1939- Volume(issue)/page/year: 52,297,1975

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赫斯特荧光燃料,33258安全信息

[ 符号 ]:

GHS07

[ 信号词 ]:
Warning

[ 危害声明 ]:
H302-H315-H319

[ 警示性声明 ]:
P305 + P351 + P338

[ 个人防护装备 ]:
dust mask type N95 (US);Eyeshields;Gloves

[ 危害码 (欧洲) ]:
Xn: Harmful;

[ 风险声明 (欧洲) ]:
R22

[ 安全声明 (欧洲) ]:
S26-S36-S24/25-S23

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ RTECS号 ]:
SM1140500

赫斯特荧光燃料,33258文献

Early life exposure to chronic intermittent hypoxia causes upper airway dilator muscle weakness, which persists into young adulthood.

Exp. Physiol. 100 , 947-66, (2015)

What is the central question of this study? Chronic intermittent hypoxia (CIH) is a dominant feature of respiratory control disorders, which are common. We sought to examine the effects of exposure to...

Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions.

PLoS ONE 10(6) , e0129961, (2015)

Current tissue engineering methods are insufficient for total joint resurfacing, and chondrocytes undergo de-differentiation when expanded on tissue culture plastic. De-differentiated chondrocytes sho...

Drosophila Gyf/GRB10 interacting GYF protein is an autophagy regulator that controls neuron and muscle homeostasis.

Autophagy 11 , 1358-72, (2015)

Autophagy is an essential process for eliminating ubiquitinated protein aggregates and dysfunctional organelles. Defective autophagy is associated with various degenerative diseases such as Parkinson ...

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