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二氢荧光黄二乙酸盐

二氢荧光黄二乙酸盐用途

Dihydrofluorescein diacetate是用于无细胞系统和细胞模型中测量氧化应激的荧光探针。

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二氢荧光黄二乙酸盐名称

[ CAS 号 ]:
35340-49-9

[ 中文名 ]:
二氢荧光黄二乙酸盐

[ 英文名 ]:
Dihydrofluorescein diacetate

[中文别名 ]:

二乙酰二氢荧光素

[英文别名 ]:

MFCD00005056
Dihydrofluorescein diacetate
2-[3,6-Diacetoxy-9H-xanth-9-yl]benzoic acid
2-[3,6-Bis(acetyloxy)-9H-xanthen-9-yl]benzoic acid
D1845
2-(3,6-diacetoxy-xanthen-9-yl)-benzoic acid
2-(3,6-Diacetoxy-xanthen-9-yl)-benzoesaeure
Diacetyldihydrofluorescein

二氢荧光黄二乙酸盐生物活性

[ 描述 ]:

Dihydrofluorescein diacetate是用于无细胞系统和细胞模型中测量氧化应激的荧光探针。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

对于许多基于细胞的研究,二氢荧光素二乙酸酯可以是优异的荧光探针。它是用于检测细胞内氧化剂的更好的荧光探针,因为它对特定的氧化物质更具反应性。二氢荧光素二乙酸酯在复氧内皮中显示线性结构的荧光,与线粒体一致[1]。二氢荧光素二乙酸酯能够检测线粒体中ROS的存在。二氢荧光素二乙酸酯荧光是尖锐的并且描绘了细丝,其对应于TMRM染色的线粒体的所有细节。它进入线粒体并与基质中释放的ROS反应[2]。二氢荧光素二乙酸酯可用于测量纳米颗粒处理细胞的氧化电位[3]。

[细胞实验]

对于无细胞研究，使用二氢荧光素二乙酸酯，终浓度为5μM。激发波长为488nm，发射波长为521nm，与共聚焦显微镜的激发和发射波长相同。所有荧光测量均在室温溶液中进行。首次传代的人脐静脉内皮在48孔板上培养至汇合。用PBS洗涤细胞，然后向细胞中加入含有葡萄糖pH7.4的PBS。加入二氢荧光素二乙酸酯，终浓度为20mM。没有细胞的孔也装有荧光探针作为对照。在Exλ488nm和Emλ510（带通5nm）和538nm（带通10nm）下每10分钟在荧光板读数器上进行荧光测量。将Emλ510nm和Emλ538nm值加在一起[1]。

[参考文献]

[1]. Hempel SL, et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med. 1999 Jul;27(1-2):146-59.

[2]. Diaz G, et al. Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes. Histochem Cell Biol. 2003 Oct;120(4):319-25.

[3]. Aranda A, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63.

[相关活性小分子]

二氢荧光黄二乙酸盐物理化学性质

[ 密度 ]:
1.353g/cm3

[ 沸点 ]:
554.3ºC at 760 mmHg

[ 熔点 ]:
213-215ºC(lit.)

[ 分子式 ]:
C₂₄H₁₈O₇

[ 分子量 ]:
418.39600

[ 精确质量 ]:
418.10500

[ PSA ]:
99.13000

[ LogP ]:
4.52130

[ 折射率 ]:
1.626

[ 储存条件 ]:
2-8℃

二氢荧光黄二乙酸盐安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 安全声明 (欧洲) ]:
S22-S24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ 海关编码 ]:
2932999099

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二氢荧光黄二乙酸盐海关

[ 海关编码 ]: 2932999099

[ 中文概述 ]:
2932999099. 其他仅含氧杂原子的杂环化合物. 增值税率:17.0%. 退税率:13.0%. 监管条件:无. 最惠国关税:6.5%. 普通关税:20.0%

[ 申报要素 ]: 品名, 成分含量, 用途

[ Summary ]:
2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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