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5-羧基荧光素琥珀酰亚胺酯

5-羧基荧光素琥珀酰亚胺酯用途

5-FAM SE是较受欢迎的绿色荧光试剂,可用于标记多肽,蛋白和核酸,可用于制备各种荧光小分子。

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5-羧基荧光素琥珀酰亚胺酯名称

[ CAS 号 ]:
92557-80-7

[ 中文名 ]:
5-羧基荧光素琥珀酰亚胺酯

[ 英文名 ]:
5-FAM SE

[英文别名 ]:

MFCD00077322
5-Carboxyfluorescein N-succinimidyl ester
5-FAM SE

5-羧基荧光素琥珀酰亚胺酯生物活性

[ 描述 ]:

5-FAM SE是较受欢迎的绿色荧光试剂,可用于标记多肽,蛋白和核酸,可用于制备各种荧光小分子。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

研究领域 >> 其他

染料试剂

[相关活性小分子]

5-羧基荧光素琥珀酰亚胺酯物理化学性质

[ 密度 ]:
1.7±0.1 g/cm3

[ 沸点 ]:
778.7±70.0 °C at 760 mmHg

[ 熔点 ]:
>300°C (dec.)

[ 分子式 ]:
C₂₅H₁₅NO₉

[ 分子量 ]:
473.388

[ 闪点 ]:
424.7±35.7 °C

[ 精确质量 ]:
473.074677

[ PSA ]:
139.67000

[ LogP ]:
0.71

[ 外观性状 ]:
黄色至橙色固体

[ 蒸汽压 ]:
0.0±2.8 mmHg at 25°C

[ 折射率 ]:
1.790

[ 储存条件 ]:
−20°C

5-羧基荧光素琥珀酰亚胺酯MSDS

详细MSDS(安全技术说明书)

5-羧基荧光素琥珀酰亚胺酯安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xi

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

5-羧基荧光素琥珀酰亚胺酯文献

A DNA-based molecular probe for optically reporting cellular traction forces.

Nature Methods 11(12) , 1229-32, (2014)

We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring defo...

DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.

Nucleic Acids Res. 20 , 2471, (1992)

The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP...

Nonradioactive labeling of RNA.

Anal. Biochem. 233 , 124, (1996)

A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a...

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