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SUC-LEU-LEU-VAL-酪氨酸-AMC

SUC-LEU-LEU-VAL-酪氨酸-AMC用途

Suc-Leu-Leu-Val-Tyr-AMC 是一种荧光底物。

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SUC-LEU-LEU-VAL-酪氨酸-AMC名称

[ CAS 号 ]:
94367-21-2

[ 中文名 ]:
N-琥珀酰基-亮氨酰-亮氨酰-缬氨酰-酪氨酸-7-胺基-4-甲基香豆素

[ 英文名 ]:
Suc-Leu-Leu-Val-Tyr-AMC

[英文别名 ]:

z-llvy-amc
suc-leu-leu-val-tyr-amc
suc-llvy-amc
suc-leu-leu-val-tyr-7-amino-4-methylcoumarin
chymotrypsin substrate iii,fluorogenic
MFCD00080248
suc-leu-leu-val-tyr-mca

SUC-LEU-LEU-VAL-酪氨酸-AMC生物活性

[ 描述 ]:

Suc-Leu-Leu-Val-Tyr-AMC 是一种荧光底物。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

多肽产品

研究领域 >> 其他

[体外研究]

Suc-Leu-Leu-Val-Tyr-AMC(Suc-LLVY)是一种膜可渗透的钙蛋白酶特异性荧光底物,肽酰-7-氨基键的pteolytic水解释放出高度荧光的7-氨基-4-甲基香豆素(AMC)部分[1]。评估TGF-β对这些底物(例如Suc-Leu-Leu-Val-Tyr-AMC)水解的影响。将胆管上皮H69细胞与10,1,0.1或0ng/mL TGF-β孵育24小时。然后在细胞溶质提取物中荧光测定底物水解。对于suc-LLVY-AMC,z-LLE-AMC和z-LLL-AMC水解,基础活性分别为1.12,8.33和14.52nmol AMC/mg蛋白质/分钟[2]。

[激酶实验]

使用相同量的蛋白质对两组样品进行免疫沉淀。在用50μMsuc-Leu测定肽酶活性之前，分别用50mM Hepes / KOH（pH7.5）和含有2mMATP的50mM Hepes / KOH（pH7.5）洗涤20S和26S蛋白酶体免疫沉淀物。 - Leu-Val-Tyr-AMC作为这些缓冲液中的底物[3]。

[参考文献]

[1]. Roberta L. Debiasi, et al. Reovirus-Induced Apoptosis Is Preceded by Increased Cellular Calpain Activity and Is Blocked by Calpain Inhibitors. J Virol. 1999 Jan; 73(1): 695–701.

[2]. Tadlock L, et al. Transforming growth factor-beta inhibition of proteasomal activity: a potential mechanism of growth arrest. Am J Physiol Cell Physiol. 2003 Aug;285(2):C277-85. Epub 2003 Mar 19.

[3]. Gardner RC, et al. Characterization of peptidyl boronic acid inhibitors of mammalian 20 S and 26 S proteasomes and their inhibition of proteasomes in cultured cells. Biochem J. 2000 Mar, 2:447-54.

[相关活性小分子]

SUC-LEU-LEU-VAL-酪氨酸-AMC物理化学性质

[ 密度 ]:
1.249g/cm3

[ 沸点 ]:
1116.8ºC at 760mmHg

[ 分子式 ]:
C₄₀H₅₃N₅O₁₀

[ 分子量 ]:
763.87600

[ 闪点 ]:
629.2ºC

[ 精确质量 ]:
763.37900

[ PSA ]:
233.24000

[ LogP ]:
5.17700

[ 折射率 ]:
1.577

[ 储存条件 ]:
−20°C

SUC-LEU-LEU-VAL-酪氨酸-AMCMSDS

详细MSDS(安全技术说明书)

SUC-LEU-LEU-VAL-酪氨酸-AMC安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

SUC-LEU-LEU-VAL-酪氨酸-AMC文献

Human high temperature requirement serine protease A1 (HTRA1) degrades tau protein aggregates.

J. Biol. Chem. 287(25) , 20931-41, (2012)

Protective proteases are key elements of protein quality control pathways that are up-regulated, for example, under various protein folding stresses. These proteases are employed to prevent the accumu...

Turnover of oxidatively damaged nuclear proteins in BV-2 microglial cells is linked to their activation state by poly-ADP-ribose polymerase.

FASEB J. 15(8) , 1460-2, (2001)

Activation of the cell death program by nitric oxide involves inhibition of the proteasome.

J. Biol. Chem. 274(28) , 19581-6, (1999)

The ubiquitin/proteasome pathway mediates the degradation of many short-lived proteins that are critically involved in the regulation of cell proliferation and cell death, including the tumor suppress...

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